4-Hydroxycoumarin CAS 1076-38-6 Ọcha>99.0% (HPLC)

4 Usoro ule
4.1 Ọdịdị (nleba anya)
4.2 Mfu na ihicha
4.3 Mkpebi nke ọdịnaya 4-Hydroxycoumarin
4.3.1 Usoro mkpebi
A gbazere samples ndị a na usoro mkpanaka wee kpebisie ike site na chromatography mmiri na-arụ ọrụ dị elu na kọlụm C18 site na usoro nhazi oke mpaghara.
4.3.2 Reagent
a) Acetonitrile: chromatographic dị ọcha (bubata);
b) Mmiri: Mmiri a na-ekpo ọkụ ma ọ bụ nke abụọ distilled mmiri.
4.3.3 Akụrụngwa
a) chromatography mmiri na-arụ ọrụ dị elu;
b) spectrometer nke na-agbanwe agbanwe nke UV;
c) Kọlụm nyocha: kọlụm C18;
d) Ultrasonic oscillators.
4.3.4 Chromatographic ọnọdụ ọrụ
a) Usoro mkpanaka: acetonitrile: mmiri: phosphoric acid = 700: 300: 1 (V / V / V);
b) Ọnụ ụzọ: 1.0ml / min;
c) Okpomọkụ kọlụm: okpomọkụ ụlọ;
d) Ogologo oge nchọpụta: 254nm
e) Nlele nha: 20μL
4.3.5 Nkwadebe nke ngwọta ule
Tulee ihe atụ 20mg (n'ụzọ ziri ezi na 0.002g), tinye ya na karama volumetric 25ml, gbarie ya na usoro mkpanaka na 4.2.4 a) na ọnụ ọgụgụ, maa jijiji nke ọma na ultrasonic shaker maka iji mee ihe.
4.3.6 Mkpebi ọdịnaya
N'okpuru ọnọdụ nke 4.3.4 n'elu, a ga-ekpebi ngwọta ule mgbe emechara ngwa ahụ.
4.3.7 Mgbakọ
Ejiri usoro nhazi mpaghara kacha elu wee gbakọọ nsonaazụ ya.
4.3.8 Ntughari ekwe omume
Ngbanwe nke nha abụọ ahụ abụghị ihe karịrị 0.5%, a na-ewerekwa uru nkezi gbakọọ dị ka nsonaazụ ule.
4.4 Mkpebi nke ebe agbaze.
5 Usoro nyocha
5.1 A ga-anwale ogbe ngwaahịa ọ bụla site na ngalaba nyocha ịdị mma dịka ihe dị na ọkọlọtọ a tupu ịhapụ ụlọ ọrụ ahụ.
5.2 Mkpebi nke batches
Otu ogbe nke ngwaahịa emepụtara site na nri nri (ihe dịka 200kg).
5.3 Nlele
A na-ahọrọ akpa abụọ (barrel) na-enweghị usoro site na nkwakọ ngwaahịa nke ọ bụla.Mgbe a na-enyocha ma gwakọta ya nke ọma, a na-ekewa ha na 50g site na usoro nke akụkụ anọ, nke a na-ekewa n'ime akụkụ abụọ, nke a na-eji otu n'ime ule nyocha ma debe nke ọzọ maka ntụle.
5.4 Mkpebi
A ga-anwale ogbe ngwaahịa ọ bụla dịka ihe ndị achọrọ n'ụkpụrụ a siri dị.Ọ bụrụ na ihe ọ bụla emezughị ihe ndị akwadoro n'ụkpụrụ a, a ga-ahapụ ya ka ọ nwagharịa site na nlele abụọ.Ọ bụrụ na a ka nwere otu ihe na-emezughị ihe ndị akwadoro n'ụkpụrụ a, a ga-ekpebi ogbe ngwaahịa na erughị eru.
5.5 Mkpezi ikpe
Onye ọrụ ahụ nwere ikike ịme nyocha nnabata n'ime ụbọchị iri na ise mgbe ọ nwetasịrị ngwongwo dịka usoro nke ọkọlọtọ a si dị.N'ọnọdụ ọ bụla mma emegideghị, akụkụ abụọ ahụ nwere ike kparịta ụka iji dozie ya, ma ọ bụ nyefee ụlọ ọrụ mkpezi ikpe nke ndị otu abụọ ahụ kwekọrịtara ka ha mee mkpezi dịka ndokwa nke ọkọlọtọ a siri dị.
6 Akara na akara
A ga-etinye akara ndị a na ngwungwu ngwaahịa:
a) Aha ngwaahịa;
b) Nọmba ọkọlọtọ;
c) Net arọ;
d) Ụbọchị mmepụta ma ọ bụ nọmba ogbe;
e) Aha na adreesị nke ụlọ ọrụ mmepụta ihe;
f) Ndụ nchekwa.