Acesulfame K CAS 55589-62-3 Acesulfame Potassium ịdị ọcha>99.0% (HPLC)

Nkọwa
Acesulfame Potassium nwere NLT 99.0% na NMT 101.0% nke C4H4NO4SK, gbakọọ na ntọala akpọnwụwo.
Nchọpụta
• A. INFRARED ABSORPATION <197K>
• B. Nnwale njirimara-General, Potassium <191>
Ngwọta nlele: 100 mg / ml
Usoro nnabata: Zuru oke achọrọ
ASSAY
• Usoro
Ihe atụ: 150 mg
Usoro Titrimetric (Lee Titrimetry <541>)
Ụdị: Direct titration
Titrant: 0.1 N perchloric acid VS
Oghere: 50 ml nke glacial acetic acid
Nchọpụta njedebe: Potentiometric
Nyocha: gbazee ihe nlele ahụ na 50 ml nke glacial acetic acid.
Titrate nwere 0.1 N perchloric acid VS.Mee mkpebi efu.
Gbakọọ pasent nke potassium acesulfame (C4H4NO4SK) na nlele:
Nsonaazụ = [(V - B) × N × F × 100]/W
V = olu titrant nke Sample (mL) na-eri.
B = olu titrant nke oghere (mL) na-eri.
N = titrant ezigbo nkịtị (mEq/ml)
F = ihe nhata, 201.2 mg/mEq
W = arọ nke Sample (mg)
Usoro nnabata: 99.0%-101.0% na ntọala akpọnwụwo
IHE adịghị ọcha
• OKWU FLUORIDE
[IHE-Jiri plasticware n'oge ule a niile.]
Ngwọta A: igbari 210 g nke citric acid monohydrate na 400 ml mmiri.Gbanwee ya na amonia gbasiri ike na pH nke 7.0, ma gbanye mmiri na 1000 ml.
Ngwọta B: 132 mg/mL nke dibasic ammonium phosphate
Ngwọta C: Na nkwusioru nke 292 g nke edetic acid na 500 ml mmiri, tinye 200 ml nke ammonium hydroxide, dozie ya na ammonium hydroxide na pH n'etiti 6 na 7, ma gbanye mmiri ka ọ bụrụ 1000 ml.
Ngwọta nchekwa: Gwakọta nha nha nha nke Ngwọta A, Ngwọta B na Ngwọta C, wee dozie ya na ammonium hydroxide na pH nke 7.5.
Ngwọta ngwaahịa ọkọlọtọ: Tulee 0.442 g nke sodium fluoride, nke a mịrị amị na 300 Celsius maka awa 12, n'ime flask volumetric 1-L, wee gbanye mmiri ka ọ bụrụ olu.Chekwaa ihe ngwọta n'ime akpa rọba mechiri emechi.Ozugbo tupu ojiji, pipet 5 ml nke ngwọta a n'ime 100-mL volumetric flask, na itughari na mmiri ka olu.Otu ml nke ngwọta a nwere 10µg nke fluoride ion.
Ngwọta ọkọlọtọ A: Gwakọta 0.5 ml nke ngwọta ngwaahịa ọkọlọtọ na 15.0 mL nke ngwọta nchekwa, wee gbanye mmiri na 50ml.
Ọkọlọtọ ngwọta B: Gwakọta 1.0 ml nke ngwọta ngwaahịa ọkọlọtọ na 15.0 ml nke ngwọta nchekwa, ma gbanye mmiri na 50ml.
Ihe ngwọta ọkọlọtọ C: Gwakọta 1.5 mL nke ngwọta ngwaahịa ọkọlọtọ na 15.0 ml nke ngwọta nchekwa, ma gbanye mmiri na 50ml.
Ihe ngwọta ọkọlọtọ D: Gwakọta 3.0 ml nke ngwọta ngwaahịa ọkọlọtọ na 15.0 mL nke ngwọta nchekwa, ma gbanye mmiri na 50ml.
Ngwọta nlele: Na flask volumetric 50-mL tinye 3 g nke Acesulfame Potassium.Gbanyụọ n'ime mmiri, gbakwunye 15.0 ml nke ngwọta nchekwa, ma jiri mmiri na-atụgharị na olu.
Nyocha
Nlereanya: Ọkọlọtọ ngwọta A, Ọkọlọtọ ngwọta B, Ọkọlọtọ ngwọta C, Ọkọlọtọ ngwọta D, na Sample ngwọta
Gwakọta otu ike (lee Titrimetry <541>) na mV, nke Standard Solution na Sample Solution, yana mita pH kwesịrị ekwesị nke nwere electrode kpọmkwem fluoride na electrode ntụaka ọlaọcha-ọlaọcha chloride.Mgbe ị na-atụ nha, bufee ihe ngwọta na beaker 25-mL, ma mikpuo electrodes.Fanye mmanya na-akpali akpali nke nwere polytef n'ime beaker, tinye beaker n'elu ihe ndọta nke nwere elu mkpuchi ma kwe ka ọ kpalie ruo mgbe a ga-enweta nha anya (1-2 min).Sachaa, ma kpochaa electrodes n'etiti nha, na-elezi anya ka ị ghara ịcha kristal n'ime ion electrode nke nwere fluoride.Tulee ike nke ngwọta Ọkọlọtọ ọ bụla, wee chepụta mkpokọta fluoride, na µg/mL, dabere na ike, na mV, na mpempe akwụkwọ semilogarithmic.Tulee ike nke ihe ngwọta Sample, wee chọpụta mkpokọta fluoride site na usoro ọkọlọtọ, na µg/mL.
Gbakọọ ọdịnaya, na ppm, nke fluoride na akụkụ nke Acesulfame Potassium were:
Nsonaazụ = (V × C/W)
V = olu nke ngwọta nlele (mL)
C = ntinye nke fluoride na ngwọta Sample, site na usoro ọkọlọtọ (mg/mL)
W = arọ nke Acesulfame Potassium were iji kwado ihe ngwọta Sample (g)
Usoro nnabata: NMT 3 ppm
• Ọla dị arọ, Usoro I <231>: NMT 10 ppm
• CHROMATỌGRAPHIC dị ọcha
Ngwọta A: 3.3 mg/mL nke tetrabutylammonium hydrogen sulfate
Usoro mkpanaaka: Acetonitrile na Ngwọta A (2:3)
Ngwọta kwesịrị ekwesị nke sistemu: 2 µg / ml nke ọ bụla nke USP Acesulfame Potassium RS na ethylparaben
Ngwọta ọkọlọtọ: 0.2 µg/mL nke USP Acesulfame Potassium RS
Ngwọta nlele: 10 mg / ml
Sistemụ chromatographic
(Lee Chromatography <621>, Kwesịrị ekwesị Sistemu.)
Ụdị: LC
Ihe nchọpụta: UV 227 nm
Ogidi: 4.6-mm × 25-cm;5-µm mbukota L1
Ọnụego mmiri: 1 ml / min
Nha igba ogwu: 20µL
Sistemu kwesịrị ekwesị
Ọmụma: Ngwọta dabara adaba sistemụ
Kwesịrị ekwesị chọrọ
Mkpebi: NLT 2 n'etiti acesulfame potassium na ethylparaben
Nyocha
Ihe atụ: Ngwọta ọkọlọtọ na ngwọta nlele
Dekọọ chromatograms maka oge ịgba ọsọ NLT ugboro 3 n'oge njide nke acesulfame potassium kacha elu, wee tụọ nzaghachi mpaghara nke ọnụ ọgụgụ kacha elu.
Usoro nnabata: Nzaghachi nke ọnụ ọgụgụ kasị elu ọ bụla n'oge njide na-abụghị nke acesulfame potassium sitere na ngwọta Sample adịghị agafe nzaghachi nke acesulfame potassium peak sitere na ngwọta Standard (0.002%).
Nnwale akọwapụtara
• Acidity ma ọ bụ ALKALINITY
Ngwọta nlele: 4.0 g na 20 ml nke mmiri na-enweghị carbon dioxide
Nyocha: Tinye 0.1 ml nke bromothymol blue TS.Ọ bụrụ na ngwọta bụ edo edo, titrate na 0.01 N sodium hydroxide iji mepụta agba anụnụ anụnụ.Ọ bụrụ na ngwọta bụ acha anụnụ anụnụ, titrate na 0.01 N hydrochloride acid iji mepụta agba odo.
Usoro nnabata: NMT 0.2 ml nke 0.01 N sodium hydroxide ma ọ bụ NMT 0.2 mL nke 0.01 N hydrochloric acid ka achọrọ.
• Ọnwụ na-ehichapụ <731>: Kpochapụ ihe nlele na 105 ° maka awa 3: ọ na-efunahụ NMT 1.0% nke ibu ya.
NKWUO GBASARA YA
• Nkwakọba na Nchekwa: Chekwaa n'ime akpa mechiri emechi nke ọma, ma chebe ya pụọ ​​​​na ìhè.Na-echekwa na ụlọ okpomọkụ.
Ụkpụrụ ntụaka USP <11>
USP Acesulfame Potassium RS