Adenine CAS 73-24-5 Assay 98.0% ~ 102.0% (Titration) Ụlọ ọrụ dị ọcha

Adenine nwere NLT 98.0% na NMT 102.0% nke C 5H5N5, gbakọọ na ntọala akpọnwụwo.
Nchọpụta
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ASSAY
Usoro
Ihe atụ: 200 mg nke Adenine
Oghere: 80 ml nke ngwakọta nke 100 ml nke glacial acetic acid na 300 ml nke acetic anhydride.
Sistemụ Titrimetric
Ụdị: Direct titration
Titrant: 0.1 N kwa chloric acid VS kwadoro dị ka ndị a: Nyefee 300 mg nke potassium biphthalate na beaker 150-mL na, site na ịkpali, gbazee na 80 ml nke ngwakọta nke 100 ml nke glacial acetic acid na 300 ml nke acetic anhydride.Titrate na kwa chloric acid ngwọta.Nke ọ bụla 20.42mg nke potassium biphthalate bụ 1 ml nke 0.1 N perchloric acid.
Nchọpụta njedebe: Potentiometric
Nyocha: gbazee Nlereanya na 80 ml nke ngwakọta nke 100 ml nke glacial acetic acid na 300 ml nke acetic anhydride site na mkpali, na titrate na Titrant.Gbakọọ pasentị Adenine (C5H5N5) na mpaghara ewepụtara:
Nsonaazụ = [(V - B) × N × F × 100]/W
V = Nlereanya olu titrant (mL)
B = Oke titrant efu (mL)
N = titrant normality (mEq/ml)
F = ihe nhata, 135.13 mg/mEq
W = arọ nke Sample (mg)
Usoro nnabata: 98.0%-102.0% na ntọala akpọnwụwo
IHE adịghị ọcha
Ihe adịghị ọcha
MKWAGHỊ ihe fọdụrụ <281>: NMT 0.1%
Ọla dị arọ, Usoro II <231>: NMT 10ppm
Ihe adịghị ọcha
Usoro
pH 7.0 phosphate buffer: Gbarie 4.54 g nke monobasic potassium phosphate n'ime mmiri iji mee 500 ml nke ngwọta.Gbarie 4.73 g nke anhydrous dibasic sodium phosphate n'ime mmiri iji mee 500 ml nke ngwọta.Gwakọta 38.9 ml nke monobasic potassium phosphate ngwọta na 61.1 ml nke dibasic sodium phosphate ngwọta.Gbanwee, ọ bụrụ na ọ dị mkpa, site n'ịgbakwụnye ngwọta sodium phosphate dibasic na pH nke 7.0.
Ọkọlọtọ ngwaahịa ngwọta: Gbanyụọ ọnụọgụ USP Adenine RS kwesịrị ekwesị na mmiri ọkụ, dị jụụ, ma gbanye ya n'ụzọ dị iche iche na mmiri iji nweta ihe ngwọta na-enwe ntinye ama ama nke 0.19 mg/mL.
Ngwọta ọkọlọtọ: Pipet 5-mL akụkụ nke ngwọta ngwaahịa Standard n'ime 100-mL volumetric flasks atọ, ma gbanye ya na 0.10 N hydrochloric acid, 0.010 N sodium hydroxide, na pH 7.0 phosphate buffer, n'otu n'otu, na olu.
Ngwọta ihe atụ: Gbarie oke Adenine kwesịrị ekwesị n'ime mmiri ọkụ, dị jụụ, ma jiri mmiri na-atụgharị ọnụ iji nweta ihe ngwọta nwere njiri mara amara nke 0.19 mg/mL.
Ngwọta ihe atụ: Gbarie oke Adenine kwesịrị ekwesị n'ime mmiri ọkụ, dị jụụ, ma jiri mmiri na-atụgharị ọnụ iji nweta ihe ngwọta nwere njiri mara amara nke 0.19 mg/mL.
Ngwọta ihe atụ: Pipet 5-mL akụkụ nke ihe ngwọta ngwaahịa n'ime 100-mL volumetric flasks atọ, na itughari na 0.10 N hydrochloric acid, 0.010 N sodium hydroxide, na pH 7.0 phosphate buffer, n'otu n'otu, na olu.
Ọnọdụ Spectrometric
(Lee Spectrophotometry na Mgbasa ọkụ <851>.)
Ụdị: UV-Vis
Oke ebili mmiri: 220-320 nm
Igwe: 1 cm
Oghere: Mmiri
Nyocha
Ihe atụ: Ngwọta ọkọlọtọ na ngwọta nlele
Nkwenye njirisi: ụdị absorptivities dị iche iche, gbakọọ na nke a mịrị amị, na ogologo oge nke nnabata kachasị, maka ụzọ ọ bụla nke ngwọta kwekọrọ ekwekọ adịghị iche karịa 2.0%.
Nnwale akọwapụtara
ỌKỤKWU LOSSON <731>: Kpochapụ ihe nlele na 110 Celsius maka awa 4: ọ tụfuru NMT 1.0% nke ibu ya.
Ọdịnaya NITROGEN, Usoro II 〈461〉: 50.2%-53.4%, gbakọọ na ntọala akpọnwụwo.
NKWUO GBASARA YA
Nkwakọ ngwaahịa na nchekwa: Chekwaa n'ime akpa mechiri emechi nke ọma.
Ụkpụrụ ntụaka USP <11>
USP Adenine RS