Adenosine CAS 58-61-7 Assay 99.0% -101.0% USP Standard Factory High Purity

Nkọwa
Adenosine nwere NLT 99.0% na NMT 101.0% nke C10H13N5O4, gbakọọ na ntọala akpọnwụwo.
Nchọpụta
• A. INFRARED ABSORPATION <197K>: NMT 0.1%
ASSAY
• ADENOSINE
Ihe nlele: 200 mg nke Adenosine na mbụ akpọnwụwo na 105 ° maka 2h
Titrimetric usoro
(Lee Titrimetry <541>)
Ụdị: Direct titration
Titrant: 0.1 N kwa chloric acid VS
Nchọpụta njedebe: Potentiometric
Nyocha: igbari na 50 ml nke glacial acetic acid na titrate na 0.1 N kwa chloric acid VS.Gbakọọ pasenti nke Adenosine (C10H13N5O4) na akụkụ ewepụtara:
Nsonaazụ = [(V - B) × N × F × 100]/W
V = Nlereanya olu titrant (mL)
B = Oke titrant efu (mL)
N = nkịtị nke titrant (mEq/ml)
F= ihe nhata: 267.25 mg/mEq
W = arọ nke ihe nlele (mg)
Usoro nnabata: 99.0% -101.0% na ntọala akpọnwụwo
• IHE adịghị ọcha
• MGBAnye ihe fọdụrụ <281>: NMT 0.1%
• Ọla dị arọ, Usoro II <231>: NMT 10 ppm
• OKWU AMMONIA
Ihe ngwọta nlele: kwụsịtụ 0,5 g na 10 ml mmiri.Kwuo maka 30 s, wee gafee nzacha na-adịghị mma.Ghichaa ihe nzacha na mmiri na 15 ml, ma jiri nzacha.
Ngwọta ọkọlọtọ: 0.4 µg/mL nke ammonium chloride n'ime mmiri
Nyocha: Na ngwọta nlele na ọkọlọtọ ọkọlọtọ tinye 0.3 mL nke alkaline mer curic–potassium iodide TS, kpuchie tubes ule, ma kwe ka guzoro maka 5 min.
Usoro nnabata: Ngwọta Sample adịghị egosipụta agba odo siri ike karịa nke Standard Solution (NMT 4 ppm nke amonia).
• Mbelata nke chloride
Ihe ngwọta nlele: kwụsịtụ 0.2 g na 10 ml mmiri.Kwuo maka 30 s, gafere n'ime nzacha na-adịghị ahụkebe, ma jiri nzacha.
Ngwọta ọkọlọtọ: 2.3 µg/mL nke sodium chloride n'ime mmiri
Nyocha: Na ngwọta nlele na 10 ml nke Standard ngwọta tinye 1 ml nke nitric acid na 1 ml nke silver nitrate TS, ma gbanye mmiri ọ bụla na 40 ml.Kwe ka ihe ngwọta guzoro maka 5 min, na-echebe site na ìhè.
Usoro nnabata: Mgbe a na-ele ya anya n'okirikiri gbara ọchịchịrị, ihe ngwọta Sample abụghị ihe mgbagwoju anya karịa ngwọta ọkọlọtọ (NMT 0.007% chloride).
• OKWU SULFATE
Ihe ngwọta nlele: kwụsịtụ 0.75 g na 15 ml mmiri.Kwuo maka 30 s, gafere n'ime nzacha na-adịghị ahụkebe, ma jiri nzacha.
Ihe ngwọta ọkọlọtọ: Tinye 0.15 ml nke 0.020 N sulfuric acid na 15 ml mmiri.
Nyocha: Na ngwọta nlele na ọkọlọtọ ọkọlọtọ tinye 2 mL nke barium chloride TS na 1 mL nke 3 N hydrochloric acid, gbanye ihe ngwọta ọ bụla na mmiri na 30 ml, na mix.Kwe ka ihe ngwọta guzoro maka nkeji 5.
Nkwenye nnabata: Ngwọta nlele abụghị turbid karịa ọkọlọtọ ọkọlọtọ (NMT 0.02% sulfate).
• OKWU IKE
Ngwọta A: 6.8 g/L nke potassium hydrogen sulfate na 3.4g/L nke tetrabutylammonium hydrogen sulfate na mmiri.Gbanwee na 2 N potassium hydroxide na pH nke 6.5.
Ngwọta B: 0.1 g / L nke ngwọta sodium azide
Usoro mkpanaaka: Ngwọta A na Ngwọta B (60:40)
Ngwọta kwesịrị ekwesị nke sistemu: 0.2 mg/mL nke ọ bụla nke Adenosine na inosine na usoro mkpanaka
Ngwọta nlele: 1.0 mg/mL nke Adenosine na usoro mkpanaka
Sistemụ chromatographic
(Lee Chromatography <621>, Kwesịrị ekwesị Sistemu.)
Ụdị: LC
Ihe nchọpụta: UV 254 nm
Ogidi: 4.6-mm × 25-cm;5-µm mbukota L1
Ọnụego mmiri: 1.5 ml / min
Nha igba ogwu: 20µL
Sistemu kwesịrị ekwesị
Nlereanya: Ngwọta dabara adaba sistemụ
Kwesịrị ekwesị chọrọ
Mkpebi: NLT 9.0 n'etiti adenosine na inosine
Ihe kpatara ọdụdụ: NMT 2.5
Ngbanwe ọkọlọtọ emetụtara: NMT 2.0%
[NOTE-Chromatograph the Sample solution, ma mezie oge ịgba ọsọ ka ọ dịkarịa ala ugboro abụọ n'oge njigide nke isi kacha elu.]
Nyocha
Ihe atụ: Ngwọta nlele
Gbakọọ pasent nke adịghị ọcha ọ bụla na akụkụ nke Adenosine were:
Nsonaazụ = (rU/rT) × 100
rU = nzaghachi kacha elu nke adịghị ọcha ọ bụla sitere na ngwọta Sample
rT = nchịkọta nzaghachi niile maka ọnụ ọgụgụ kacha elu sitere na ngwọta Sample
Usoro nnabata
Ihe adịghị ọcha n'otu n'otu: NMT 0.1% nke ọ bụla nke gunosine, inosine, na uridine, yana NMT 0.2% nke adenine.
Mkpokọta adịghị ọcha: NMT 0.5%
Nnwale akọwapụtara
• ONYE NA-agbaze ma ọ bụ okpomọkụ <741>: 233°-238°
• Ntụgharị anya, Ntụgharị a kapịrị ọnụ <781S>: -68° ruo -72°
Ngwọta ule: 20 mg / mL na sodium hydroxide ngwọta (1 na 20), kpebisiri ike na ihe nlele mbụ akpọnwụwo na 105 ° maka 2h
ACIDITY MA ọ bụ ALKALINITY: Kwụsị 1 g na 20 ml nke mmiri na-enweghị carbon dioxide.Kwuo maka 30 s, wee gafee nzacha na-adịghị mma.Na nke ọ bụla n'ime akụkụ abụọ 10-mL nke filtrate tinye 0.1mL nke bromocresol purple TS.
Usoro nnabata: NMT 0.3 ml nke 0.01 N sodium hydroxide ka achọrọ iji mepụta agba anụnụ anụnụ-violet n'otu akụkụ.NMT 0.1 ml nke 0.01 N hydrochloric acid ka achọrọ iji mepụta agba odo na akụkụ nke ọzọ.
• Mkpọnwụ ọnwụ <731>: Kpochapụ ihe nlele na 105 ℃ maka awa 2: ọ na-efunahụ NMT 0.5% nke ịdị arọ ya.
NKWUO GBASARA YA
• Nkwakọba na Nchekwa: Chekwaa n'ime akpa siri ike, nke na-enweghị ọkụ, ma chekwaa na ụlọ okpomọkụ a na-achịkwa.
• Ụkpụrụ ntụaka USP <11>
USP Adenosin RS