4-Amino-5-Chloro-2,3-Dihydrobenzofuran-7-Carboxylic Acid CAS 123654-26-2 Prucalopride Succinate Intermediate Ọcha ≥98.0% (HPLC)

Ngwaahịa a bụ 4-Amino-5-Chloro-2,3-Dihydrobenzofuran-7-Carboxylic Acid, ọdịnaya nke C12H12ClNO4 agaghị abụ ihe na-erughị 98.0%, gbakọọ na anhydrous ndabere.
[Properties] Ngwaahịa a na-acha ọcha na-acha uhie uhie siri ike.
[Lelee]
Were ihe kwesịrị ekwesị nke ngwaahịa a (ihe dị ka 0.5g) mmiri, gbakwunye ihe mgbaze (methanol: N, N-dimethylformamide = 50:50), ma gbazee ya dịka usoro mkpebi mmiri si dị (Ihe mgbakwunye Ⅷ M, ụzọ mbụ A).Jiri ule Fisher wee chọpụta ruo mgbe ngwọta ya gbanwere site na ìhè edo edo gaa na-acha ọbara ọbara-aja aja na-egosi njedebe njedebe, wee mee ule ọzọ efu.Gbakọọ dịka usoro a si dị: ọdịnaya mmiri ekwesịghị gafere 1.00%.
Ọdịnaya mmiri (%) = (AB) F/W×100%
Ebe, A bụ olu mmiri mmiri Fisher nke ngwaahịa nnwale ahụ riri (ml);
B bụ olu nke mmiri ule Fisher nke oghere oghere na-eri, ml;
F bụ ịdị arọ nke ngwọta ule Fisher dabara na mmiri kwa 1ml, mg;
W bụ arọ nke ngwaahịa nnwale, mg
Ihe metụtara ya
Dị ka usoro dị n'okpuru "Ihe nleba anya ọdịnaya", ọ bụrụ na enwere ọnụ ọgụgụ adịghị ọcha na chromatogram nke ngwọta ule, ọnụ ọgụgụ kasị elu nke otu adịghị ọcha agaghị adị karịa ugboro 10 (1.0%) nke mpaghara kachasị elu nke njikwa. ngwọta, na nchikota nke ọnụ ọgụgụ kachasị elu nke adịghị ọcha niile agaghị adị karịa ugboro 20 (2.0%) nke ebe kachasị elu nke ngwọta nchịkwa.
[Mkpebi ọdịnaya] Dị ka chromatography mmiri na-arụ ọrụ dị elu (Ihe mgbakwunye VI D).
A na-eji Octadecylsilane bonded silica gel mee ihe dị ka ihe ndochi maka ọnọdụ chromatographic yana ule dabara adaba sistemụ.A na-eji ngwọta phosphate buffer (1.36g potassium dihydrogen phosphate, ultrasonic gbazere na mmiri 1L, phosphoric acid gbanwee na PH 3.0) dị ka usoro mkpanaka A. Acetonitrile bụ usoro mkpanaaka B, a na-eme gradient elution dị ka tebụl na-esonụ:
Oge (Nkeji) Usoro mkpanaaka A usoro mkpanaaka B
080 20
15 80 20
30 50 50
45 50 50
Ogologo obosara nke nchọpụta bụ 254 nm.Ọnụego ya bụ 1.0 ml / min.Ọnụ ọgụgụ nke efere usoro iwu abụghị ihe na-erughị 2000 dị ka ọnụ ọgụgụ kasị elu nke 4-Amino-5-Chloro-2,3-Dihydrobenzofuran-7-Carboxylic acid si dị.
Ọnụ ọgụgụ kasị elu nke furanoformic acid erughị 2000.
Usoro mkpebi:
Were 10mg nke ngwaahịa ule, tụọ ya n'ụzọ ziri ezi, tinye ya na karama 10ml, gbarie ya ultrasonic na mobile frasi A-mobile frasi B (70:30) dị ka ihe mgbaze, itughari ya na ọnụ ọgụgụ, maa jijiji nke ọma, na-akwadebe a. ngwọta na ntinye nke 1mg / ml.A na-agbanye 20 l nke ziri ezi n'ime chromatograph mmiri mmiri, ma dekọọ chromatogram.Isi ịdị ọcha kacha elu erughị 98.0% dịka usoro nhazi mpaghara kacha elu si dị
Ogidi: Dionex Acclaim C18, 4.6*250mm, 5um
Okpomọkụ kọlụm: 40 ℃
Ọsọ ọsọ: 1.0ml/min
Ogologo oge nchọpụta: 254nm
Nlele nha: 10μL