L-Glutamic Acid α-Benzyl Ester CAS 13030-09-6 (H-Glu-OBzl) Nyocha > 98.5% (HPLC) Ogo dị elu

1. Ịdị ọcha (HPLC)
1.1 Ngwa
chromatography mmiri mmiri dị elu, ihe nchọpụta UV3000.
Ntụle nyocha eletrọnịkị
1.2 Reagent
Acetonitrile (ọkwa chromatographic), TFA (ọkwa chromatographic)
1.3 Ọnọdụ chromatographic
1.3.1 Ogidi: kọlụm C18, 5 μl * 250 * 4.6 mm
1.3.2 Ogologo oge nchọpụta: 220nm
Ọnụego mmiri: 1.0 ml / min
Olu ịgba ọgwụ: 10 μL (maka ntụaka)
Ihe ngwọta: acetonitrile
Oge ịgba ọsọ: nkeji 20
1.4 Mobile usoro nkwadebe
Usoro mkpanaka A (0.1% Trifluoroacetic acid Water): banye n'ụzọ ziri ezi 1.0 ml TFA diluted na 1000 ml na mmiri, mix na degas.
Usoro mkpanaaka B (0.1% Trifluoroacetic acid Acetonitrile): banye 1.0 ml TFA nke ọma, tinye ya na 1000 ml na acetonitrile, mix na degas.
1.5 Mmemme gradient
Oge (nkeji) A% B%
0:00 90 10
10.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
1.6 Nkwadebe nke ngwọta sample
Were 0.1 g sample na igbari ya na acetonitrile na itughari ya na 100ml.Ma jijiji nke ọma maka ojiji n'ọdịnihu, ma ọ bụ otu itinye uche.Kwadebe ihe atụ abụọ n'otu aka ahụ.
1.7 Nnwale mkpebi
Nyochaa ihe nlele ahụ dịka usoro nlele ndị a si dị:
1) Ihe kariri ogwu ogwu 1 nke oghere oghere
2) 1 injection nke ngwọta sample 1 #
3) 1 ogwu ogbugba nke sample ngwọta 2 #
1.8.0 Mgbakọ
1.8.1 A na-eji usoro nhazi mpaghara kacha elu iji gbakọọ ịdị ọcha nke HPLC.
1.8.2 Ntugharị ikwu nke ịdị ọcha nke agịga abụọ agaghị adị karịa 1%
1.8.3 Ọ bụrụ na nsonaazụ nke injections abụọ ahụ na-agbaso ụkpụrụ nnabata, were nkezi ịdị ọcha dị ka nsonaazụ ikpeazụ.