Vardenafil Intermediate CAS 224789-21-3 Ịdị ọcha> 99.0% (HPLC)

1. Ọdịdị: Tinye sample na akwụkwọ ọcha ma hụ ọdịdị ahụ, na-acha ọcha na-acha odo odo na-acha odo odo.
2. Nyocha:
2.1 Mkpebi mgbaze dị ka iwu siri dị (Chinese Pharmacopoeia 2010 Edition II Appendix ⅥC) Ebe mgbaze bụ 142.0~148.0℃.
2.2 Mfu na ihicha: Mkpebi dị ka iwu siri dị (Ihe Okike ⅧL, Nkebi II, 2010 Mbipụta nke Chinese Pharmacopoeia).Nkenke na-atụle ego kwesịrị ekwesị nke ngwaahịa a, akọrọ na 102 ℃ maka awa 3, ọnwụ dị arọ agaghị abụ karịa 1.00%.
2.3 Ọla dị arọ: Mkpebi dị ka iwu siri dị (People's Republic of China Pharmacopoeia 2010 Edition, Part II Appendix ⅧH) Mkpebi site na usoro nke abụọ.
Nkenke na-atụ 1.0g nke ngwaahịa ule ahụ, jiri nwayọọ nwayọọ na-ere ọkụ ruo mgbe carbonized kpamkpam, dị jụụ, tinye 0.5-1.0ml sulfuric acid, mee ka ọ dị nro, kpoo ọkụ na obere okpomọkụ ruo mgbe sulfuric acid gwụchara, tinye 0.5ml nitric acid, uzuoku akọrọ, ruo mgbe. The nitrogen oxide vepor na-agwụ ike, dị jụụ, na-ekpo ọkụ na 500-600 ℃ ka carbonized kpamkpam, dị jụụ, tinye 2ml hydrochloric acid, Mgbe ihicha na mmiri ịsa ahụ, tinye 15ml mmiri, dobe ihe ngwọta amonia ruo mgbe ọ na-anọpụ iche na phenolphthalein egosi. ngwọta, tinye 2ml acetate buffer (ph3.5), na ntakịrị okpomọkụ igbari, bugharịa ya na Nachner colorimetric tube, na-atụgharị ya n'ime 25ml na mmiri.Were reagent ọzọ maka ịkwadebe ngwọta ule, wee kpoo ya na efere poselin, tinye 2ml acetate buffer (PH3.5) na 15ml mmiri ka ọ bụrụ obere okpomọkụ Mgbe mgbasa, kpalie Nath colorimetric tube, tinye ụfọdụ ego nke ọkọlọtọ ndu ngwọta, wee gbanye mmiri na 25ml.A na-eme nnwale Colorimetric n'etiti ngwọta nnwale na ngwọta ọkọlọtọ iji nweta.Standard ngwọta nkwadebe dị ka (People's Republic of China Pharmacopoeia 2010 mbipụta II Appendix Ⅷ Akwa metal ule usoro) mbụ usoro.Ọla dị arọ agaghị agafe akụkụ 20 kwa nde.
3. Ịdị ọcha: Dị ka elu arụmọrụ chromatography mmiri mmiri, kpebisiri ike dị ka iwu (Chinese Pharmacopoeia 2010 mbipụta II Mgbakwunye VD).
Ọnọdụ chromatographic:
Ogidi: C18, 200mm × 4.6mm, 5μm;
Usoro mkpanaaka: ihe nchekwa phosphate (tuo 2.72g potassium dihydrogen phosphate, gbakwunye mmiri ka ọ gbazee na itughari na 1000ml, mezie pH=3.5) : acetonitrile: = 35:65;
Ogologo oge nchọpụta: 254nm;
Ọsọ ọsọ: 1.0ml/min.
Usoro mkpebi: Were ihe dịka 30mg nke ngwaahịa a, tụọ ya nke ọma, gbanye ya na usoro mkpanaka iji mee ka ịta ihe dị ka 0.3mg/ml, were 20μl n'ime chromatograph mmiri mmiri, dekọọ chromatogram.Dabere na usoro nhazi mpaghara, ịdị ọcha adịghị erughị 99.0%.