Chenodeoxycholic Acid (CDCA) CAS 474-25-9 Nyocha ≥98% (Akọrọ Basic)

Chenodeoxycholic acid
C24H40O4: 392.57
3a,7a-Dihydroxy-5b-cholan-24-oic acid [474-25-9]
Chenodeoxycholic acid, mgbe akpọnwụwo, nwere ihe na-erughị 98.0z na ọ bụghị ihe karịrị 101.0% nke C24H40O4.
Nkọwa Chenodeoxycholic acid pụtara dị ka ọcha, kristal, crystalline ntụ ntụ ma ọ bụ ntụ ntụ.
Ọ na-agbaze n'efu na methanol yana na ethanol (99.5), soluble na acetone, yana ihe fọrọ nke nta ka ọ bụrụ ihe a na-apụghị ịsacha na mmiri.
Kpebie ụdị nnabata infrared nke Chenodeoxycholic Acid, nke a mịrị amị na mbụ, dị ka eduzi ya na usoro diski bromide n'okpuru Infrared Spectrophotometry <2.25>, ma were ụdịdị dị iche iche na Reference Spectrum: ụdịdị abụọ ahụ na-egosipụta ike nke absorption n'otu ọnụọgụ ebili mmiri ahụ.
Ntugharị anya <2.49> [a] 20D: +11.0 °~ +13.0° (mgbe ihichachara, 0.4 g, ethanol (99.5), 20 ml, 100 mm.
Ebe mgbaze <2.60> 164 ~ 169 ℃
Ịdị ọcha (1) Chloride <1.03> - Gbanyụọ 0.36 g nke Chenodeoxycholic Acid na 30 ml nke methanol, tinye 10 ml nke nitric acid na mmiri iji mee 50 ml, wee jiri ngwọta a mee ule ahụ.Kwadebe ngwọta nchịkwa dị ka ndị a: Na 1.0 ml nke 0.01 mol / L hydrochloric acid VS tinye 30 ml nke methanol, 10 ml nke nitric acid dilute na mmiri iji mee 50 ml (ọ bụghị karịa 0.1%).
(2) Ọla dị arọ <1.07>-Gaa na 1.0 g nke Chenodeoxycholic Acid dịka usoro 4 siri dị, wee mee ule ahụ.Kwadebe ngwọta nchịkwa na 2.0 ml nke Standard Lead Solution (ọ bụghị karịa 20 ppm).
(3) Barium-To 2.0 g nke Chenodeoxycholic Acid tinye 100 ml mmiri, na obụpde maka 2 nkeji.Na ngwọta a, tinye 2 ml nke hydrochloric acid, obụpde maka nkeji 2, yochaa mgbe ọ dị jụụ, wee saa nzacha na mmiri ruo mgbe ị ga-enweta 100 ml nke nzacha.Na 10 ml nke filtrate tinye 1 ml nke sulfuric acid dilute: ọ dịghị turbidity pụtara.
(4) Ihe ndị metụtara ya - gbazee 0.20 g nke Chenodeoxycholic Acid na ngwakọta nke acetone na mmiri (9: 1) iji mee kpọmkwem 10 ml, ma jiri ngwọta a dị ka ngwọta nlele.Iche iche, igbari 10 mg nke lithocholic acid maka chromatography nke dị nro na ngwakọta nke acetone na mmiri (9: 1) iji mee kpọmkwem 10 ml.Pipet 2 ml nke ihe ngwọta a, gbakwunye ngwakọta nke acetone na mmiri (9: 1) iji mee kpọmkwem 100 ml, ma jiri ngwọta a dị ka ọkọlọtọ ọkọlọtọ (1).Iche iche, igbari 10 mg nke ursodeoxycholic acid na ngwakọta nke acetone na mmiri (9: 1) mee kpọmkwem 100 ml, na-eji nke a ngwọta dị ka ọkọlọtọ ngwọta (2).Iche iche, igbari 10 mg nke cholic acid maka mkpa-layer chromatography na ngwakọta nke acetone na mmiri (9:1) ime kpọmkwem 100 ml, na-eji nke a ngwọta dị ka ọkọlọtọ ngwọta (3).Pipet 1 mL nke ngwọta nlele, ma gbakwunye ngwakọta nke acetone na mmiri (9: 1) iji mee kpọmkwem 20 ml.Pipet 0.5 ml, 1 ml, 2 ml, 3 ml na 5 ml nke ngwọta a, tinye ngwakọta nke acetone na mmiri (1) na nke ọ bụla n'ime ha iji mee kpọmkwem 50 ml, na-akọwapụta ngwọta ndị a dị ka ọkọlọtọ ngwọta A, ọkọlọtọ ngwọta. B, ọkọlọtọ ngwọta C, ọkọlọtọ ngwọta D na ọkọlọtọ ngwọta E, n'otu n'otu.Jiri ngwọta ndị a mee nnwale ahụ dị ka a gwara gị n'okpuru Chromatography nke Thin-Layer<2.03>Ntụpọ 5 mL nke ọ bụla n'ime ngwọta nlele, ọkọlọtọ ọkọlọtọ (1), (2), (3) na ngwọta ọkọlọtọ A, B, C, D na E na efere nke silica gel maka chromatography nke dị nro.Mepụta efere ahụ na ngwakọta nke 4-methyl-2-pentanone, toluene na formic acid (16: 6: 1) n'ebe dị anya nke ihe dịka 15 cm, kpochaa efere ahụ ma kpoo ya na 1209C maka nkeji 30.Ngwa ngwa, fesa ihe ngwọta nke phosphomolybdic acid n-hydrate na ethanol (95) (1 na 5) na efere, na okpomọkụ na 120 ℃ maka nkeji 2 ruo 3: ntụpọ kwekọrọ na ntụpọ ahụ nwere ngwọta ọkọlọtọ (1) adịghị ike karịa ebe nwere ọkọlọtọ ọkọlọtọ (1), ntụpọ kwekọrọ na ntụpọ ahụ nwere ọkọlọtọ ọkọlọtọ (2) adịghị ike karịa ebe nwere ọkọlọtọ ọkọlọtọ (2), na ntụpọ kwekọrọ na ntụpọ ahụ. na ọkọlọtọ ngwọta (3) adịghị ike karịa ntụpọ nwere ọkọlọtọ ngwọta
(3).Dị ka atụnyere tụrụ tụrụ na ọkọlọtọ ngwọta A, B, C, D na E, ntụpọ ndị ọzọ karịa isi ntụpọ na ndị ọzọ karịa tụrụ ndị a kpọtụrụ aha n'elu adịghị ike karịa ntụpọ na ọkọlọtọ ngwọta E, na ngụkọta ego. n'ime ha abụghị ihe karịrị 1.5%.
Ọnwụ na ihicha <2.41> Ọ bụghị ihe karịrị 1.5% (1 g, 105 ℃, 3 awa).
Ihe fọdụrụ na mgbanye <2.44> Ọ bụghị karịa 0.1% (1 g).
Assay tụọ n'ụzọ ziri ezi banyere 0.5 g nke Chenodeoxycholic Acid, nke a mịrị amị, gbarie na 40 ml ethanol (95) na 20 ml mmiri, yana titrate <2.50> na 0.1 mol/L sodium hydroxide VS (potentiometric titration).Mee mkpebi efu n'otu ụzọ ahụ, ma mee mgbazi ọ bụla dị mkpa.
Otu ml nke 0.1 mol/L sodium hydroxide VS ＝ 39.26 mg nke C24H40O4
Ebube na ihe nchekwa-akpa ndị siri ike.