Chloramphenicol CAS 56-75-7 Dị Ọcha ≥99.0% (HPLC) ịdị ọcha dị elu

Chloramphenicol
C11H12Cl2N2O5 323.13 [56-75-7].
Chloramphenicol nwere ihe na-erughị 97.0 pasent na ọ bụghị karịa 103.0 pasent nke C11H12Cl2N2O5.
Nkwakọ ngwaahịa na nchekwa - Chekwaa n'ime akpa siri ike.
Labeling-Ebe e bu n'obi ka e jiri ya mee ihe n'ịkwadebe ụdị ọgwụgwọ injectable ma ọ bụ ihe ndị ọzọ na-adịghị mma, akara ahụ na-ekwu na ọ dịghị mma ma ọ bụ na a ga-edozi ya n'ihu nhazi n'oge nkwadebe nke injectable ma ọ bụ ndị ọzọ na-edozi usoro onunu ogwu.
Ụkpụrụ ntụaka USP <11>-
USP Chloramphenicol RS Pịa ka ilele Ọdịdị
USP Endotoxin RS
njirimara-
A: Infrared absorption <197K>.
B: Oge njide nke nnukwu ọnụ ọgụgụ dị elu na chromatogram nke nkwadebe Assay kwekọrọ na nke ahụ na chromatogram nke nkwadebe Standard, dị ka enwetara na Assay.
Oke agbaze <741>: n'etiti 149 na 153.
Ntụgharị a kapịrị ọnụ <781S>: n'etiti +17.0 na +20.0.
Ngwọta ule: 50 mg, akpọghị nkụ, kwa mL, na mmanya na-egbu egbu.
Crystallinity <695>: na-emezu ihe achọrọ.
Endotoxins nke nje bacteria 85-Ebe Chloramphenicol echere maka iji na-akwadebe ụdị ọgwụgwọ injectable, ọ nwere ihe karịrị 0.2 USP Endotoxin Unit kwa mg nke chloramphenicol.
Sterilite 71- Ebe akara ahụ na-ekwu na Chloramphenicol adịghị ike, ọ na-emezu ihe achọrọ mgbe a nwalere ya ka akwadoro maka Membrane Filtration n'okpuru Nnwale maka Ịdị Ike nke Ngwaahịa a ga-enyocha, ma e wezụga iji 1 g nke ụdị ihe siri ike.
pH <791>: n'etiti 4.5 na 7.5, na nkwusioru mmiri nwere 25 mg kwa ml.
Ịdị ọcha Chromatographic - Gbazee ọnụọgụ Chloramphenicol nke a tụrụ nke ọma na methanol iji nweta ngwọta nnwale nwere 10 mg kwa ml.Kwadebe ihe ngwọta nke USP Chloramphenicol RS na methanol nwere 10 mg kwa ml (Standard solution A).Jiri methanol gbanye akụkụ nke ihe ngwọta ọkọlọtọ A nke ọma iji nweta ngwọta ọkọlọtọ B nwere 100 µg kwa ml yana ọkọlọtọ ọkọlọtọ C nwere 50 μg kwa ml.Tinye akụkụ 20-µL dị iche iche nke ngwọta nnwale yana ọkọlọtọ ọkọlọtọ B na C gaa na efere chromatographic dị gịrịgịrị dabara adaba (lee Chromatography 621), kpuchie ya na akwa 0.25-mm nke ngwakọta silica chromatographic chromatographic.Zụlite chromatogram n'ime usoro ihe mgbaze nke nwere ngwakọta nke chloroform, methanol, na glacial acetic acid (79:14:7) ruo mgbe ihu ihe mgbaze na-ebugharị ihe dị ka ụzọ atọ n'ụzọ anọ nke ogologo efere ahụ.Wepu efere ahụ n'ime ụlọ ahụ, kpoo ikuku, wee nyochaa n'okpuru ọkụ UV dị mkpụmkpụ: ntụpọ ọ bụla na-abụghị isi ebe a na-enweta site na ngwọta ule anaghị agafe n'ogo ma ọ bụ ike isi ebe a nwetara site na Standard Solution B (1% ), na nchikota nke adịghị ọcha na-anọchi anya ntụpọ niile na-abụghị isi ntụpọ, dabere na ntụnyere ike nke ntụpọ dị otú ahụ na ike nke isi ntụpọ enwetara site na Standard Solution B na C, anaghị agafe 2% .
Nyocha -
Usoro mkpanaaka - Kwadebe ngwakọta mmiri, methanol, na glacial acetic acid kwesịrị ekwesị (55:45:0.1).Mee mgbanwe ọ bụrụ na ọ dị mkpa (lee Dabara Sistemu n'okpuru Chromatography 621).
Nkwadebe ọkọlọtọ- Gbanyụọ ọnụọgụ USP Chloramphenicol RS n'ụzọ ziri ezi na usoro mkpanaaka, wee tụgharịa n'ụzọ dị iche iche, yana n'usoro ma ọ bụrụ na ọ dị mkpa, yana usoro mkpanaaka iji nweta ihe ngwọta nwere njiri mara nke ihe dịka 80 µg kwa ml.Wepụta akụkụ nke ngwọta a site na nzacha 0.5-µm ma ọ bụ mma porosity, wee jiri nzacha doro anya dị ka nkwadebe ọkọlọtọ.
Nkwadebe nyocha- Nyefee ihe dị ka 200 mg nke Chloramphenicol, tụọ ya nke ọma, na flask volumetric 100-mL, tinye usoro mkpanaka na olu, ma gwakọta.Nyefee 4.0 ml nke ihe ngwọta a ga-esi na ya pụta na flask volumetric 100-mL, gbanye ya na usoro Mobile ka ọ bụrụ olu, na mix.Wepụta akụkụ nke ngwọta a site na nzacha 0.5-µm ma ọ bụ mma porosity, ma jiri nzacha doro anya dị ka nkwadebe Assay.
Usoro Chromatographic (lee Chromatography <621>) -Kromatograph mmiri mmiri nwere ihe nchọta 280-nm yana kọlụm 4.6mm × 10-cm nke nwere 5-µm mbukota L1.Ọnụego mmiri ahụ bụ ihe dịka 1 ml kwa nkeji.Chromatograph the Standard nkwadebe, wee dekọọ nzaghachi kacha elu dị ka a na-eduzi n'okpuru Usoro: arụmọrụ kọlụm ekpebisiri ike site na ọnụ ọgụgụ nyocha erughị 1800 efere usoro ihe omume, ihe ọdụdụ ahụ abụghị ihe karịrị 2.0, yana ndakọrịta ọkọlọtọ maka injections ugboro ugboro abụghị ihe karịrị 2.0. karịa 1.0%.
Usoro-[ ndetu-Jiri elu elu ebe egosiri nzaghachi kacha elu.] Kewapụ iche, gbanye nha nha nha (ihe dịka 10 µL) nke nkwadebe Standard na nkwadebe Assay n'ime chromatograph, dekọọ chromatogram, ma tụọ azịza maka ọnụ ọgụgụ ndị bụ isi.Gbakọọ ọnụọgụ, na mg, nke C11H12Cl2N2O5 n'ime akụkụ Chloramphenicol nke usoro a were were:
2.5C (rU / rS)
nke C bụ ntinye uche, na µg kwa ml, nke USP Chloramphenicol RS na nkwadebe Standard, na rU na rS bụ nzaghachi kachasị elu nke enwetara site na nkwadebe Assay na Standard nkwadebe, n'otu n'otu.