Citric Acid Anhydrous CAS 77-92-9 Assay 99.5 ~ 100.5%

Citric acid [77-92-9].
Nkọwa
Anhydrous Citric Acid nwere NLT 99.5% na NMT 100.5% nke C6H8O7, gbakọọ na anhydrous ndabere.
Nchọpụta
• Ntinye infrared <197K>: Kpochapụ ihe a ga-enyocha na 105 ℃ maka awa 2.
ASSAY
• Usoro
Ihe atụ: 0.550 g nke Citric Acid Anhydrous;dekọọ ibu nke ọma.
Nyocha: igbari Sample na 50 ml mmiri.Tinye 0,5 ml nke phenolphthalein TS.Titrate nwere 1 N sodium hydroxide VS.ML nke ọ bụla nke 1 N sodium hydroxide bụ 64.03 mg nke C6H8O7.
Usoro nnabata: 99.5% -100.5% na ndabere anhydrous
IHE adịghị ọcha
Ihe adịghị ọcha
• Ihe fọdụrụ na mgbanye <281>: NMT 0.1%, kpebisiri ike na 1.0 g
• Ọla dị arọ <231>: NMT 10 ppm
• Sulfate
Ihe ngwọta sulfate ọkọlọtọ A: 1.81 mg/mL nke potassium sulfate na mmanya 30%.Ozugbo tupu ojiji, nyefee 10.0 ml nke ngwọta a na 1000-mL volumetric flask, gbanye na 30% mmanya na olu, na mix.Ihe ngwọta a nwere 10 μg / ml nke sulfate.
Ọkọlọtọ sulfate ngwọta B: 1.81 mg/mL nke potassium sulfate na mmiri.Ozugbo tupu ojiji, nyefee 10.0 ml nke ngwọta a na 1000-mL volumetric flask, gbanye na mmiri na olu, na mix.Ihe ngwọta a nwere 10 μg / ml nke sulfate.
Ihe ngwọta ngwaahịa: 66.7 mg/mL nke citric acid
Ngwọta nlele: Na 4.5 ml nke Standard sulfate solution A, tinye 3 mL nke ngwọta barium chloride (1 n'ime 4), maa jijiji, ma kwe ka guzoro maka 1 min.Na 2.5 ml nke nkwusioru a ga-esi na ya pụta, tinye 15 ml nke ngwọta ngwaahịa Sample na 0.5 ml nke 5 N acetic acid, na mix.
Ọkọlọtọ ngwọta: Kwadebe dị ka akwadoro maka ngwọta Sample, belụsọ iji 15 ml nke Standard sulfate solution B kama iji ngwọta ngwaahịa Sample.
Nyocha
Ihe atụ: Ngwọta ọkọlọtọ na ngwọta nlele
Nkwenye nnabata: Ọgba aghara ọ bụla a na-emepụta na ngwọta Sample mgbe 5 min guzoro adịghị elu karịa nke emepụtara na Standard Solution (0.015%).
• Oke nke Aluminom (ebe akpọnyere ya dị ka echere maka ojiji na dialysis)
Ihe ngwọta aluminom ọkọlọtọ: Iji 352 mg nke aluminom potassium sulfate na 100-mL volumetric flask, gbakwunye mmiri ole na ole, swirl ka igbari, tinye 10 mL nke sulfuric acid diluted, gbanye mmiri na olu, na mix.Ozugbo tupu ojiji, gbanye 1.0 ml nke ngwọta a na mmiri na 100.0 ml.
pH 6.0 acetate buffer: igbari 50 g nke ammonium acetate na 150 ml mmiri, gbanwee ya na glacial acetic acid na pH nke 6.0, gbanye mmiri na 250 ml, na mix.
Ngwọta ọkọlọtọ: Kwadebe ngwakọta nke 2.0 ml nke ngwọta aluminom ọkọlọtọ, 10 mL nke pH 6.0 acetate buffer, na 98 ml mmiri.Wepụ ngwakọta a dị ka akọwara maka ihe ngwọta Sample, gbanye ihe ndị agbakwunyere na chloroform na olu, ma gwakọta ya.
Ngwọta nlele: Gbarie 20.0 g nke Anhydrous Citric Acid na 100 ml mmiri, tinye 10 ml nke pH 6.0 acetate buffer.Wepụta ihe ngwọta a na akụkụ nke 20, 20, na 10 mL nke 0.5% ngwọta nke 8-hydroxyquinoline na chloroform, na-ejikọta ihe ndị na-emepụta chloroform na flask volumetric 50-mL.Jiri chloroform gwakọta ihe ndị a jikọtara ọnụ na olu, ma gwakọta ya.
Ihe ngwọta efu: Kwadebe ngwakọta nke 10 ml nke pH 6.0 acetate buffer na 100 ml mmiri.Wepụ ngwakọta a dị ka akọwara maka ihe ngwọta Sample, jiri chloroform mee ka ihe ndị agbakwunyere na-agbakọta na olu, ma gwakọta ya.
Ọnọdụ fluorometric
Ogologo oge mkpali: 392 nm
Ogologo ikuku ikuku: 518 nm
Nyocha
Ihe atụ: Ngwọta ọkọlọtọ na ngwọta nlele
Kpebisie ike na ọkụ ọkụ nke Samples na fluorometer setịpụrụ dị ka eduzi n'okpuru ọnọdụ Fluorometric, na-eji oghere oghere iji tọọ ngwa ahụ na efu.
Usoro nnabata: Igwe ọkụ nke ihe ngwọta Sample adịghị agafe nke ngwọta Standard (0.2 ppm).
Ihe adịghị ọcha
• Usoro: Oke Oxalic Acid
Ihe ngwọta ngwaahịa: 200 mg/mL nke Citric Acid Anhydrous n'ime mmiri
Ngwọta nlele: Na 4 ml nke ihe ngwọta ngwaahịa, tinye 3 ml nke hydrochloric acid na 1 g nke zinc granular, sie maka nkeji 1, ma kwe ka guzoro maka 2 min.Nyefee ihe dị elu na tube ule nwere 0.25 ml nke ngwọta phenylhydrazine hydrochloride (1 n'ime 100), ma kpoo ọkụ.Jụọ ngwa ngwa, bufee na cylinder nke gụsịrị akwụkwọ, ma tinye nha nha nke hydrochloric acid na 0.25 ml nke ngwọta ferricyanide potassium (1 n'ime 20).Kpoo, ma kwe ka o guzoro maka nkeji iri atọ.
Ọkọlọtọ ngwọta: Kwadebe dị ka akwadoro maka ihe ngwọta Sample, ewezuga iji 4 ml nke 0.10 mg/mL oxalic acid solution, nke dabara na 0.0714 mg/mL nke oxalic acid anhydrous, kama ngwọta ngwaahịa Sample.[Rịba ama - Kwadebe otu oge na ngwọta nlele.]
Nyocha
Ihe atụ: Ngwọta ọkọlọtọ na ngwọta nlele
Ụkpụrụ nnabata: Agba pink ọ bụla emepụtara na ngwọta Sample adịghị ike karịa nke emepụtara na Standard Solution (0.036%).
Nnwale akọwapụtara
• Nnwale Endotoxins nke nje bacteria 85: Ọkwa nke endotoxins nke nje bụ na enwere ike izute ihe achọrọ n'ụdị monograph (s) dị mkpa nke ejiri Anhydrous Citric Acid mee ihe.Ebe akara ahụ na-ekwu na a ga-edobe Anhydrous Citric Acid n'ihu nhazi n'oge nkwadebe nke ụdị ọgwụgwọ injectable, ọkwa nke endotoxins nke nje bụ ihe achọrọ n'ụdị monograph (s) dị mkpa nke ejiri Anhydrous Citric Acid mee ihe. zutere.
• Ngwọta doo anya
[Rịba ama-A ga-atụnyere ihe ngwọta nlele ahụ na nkwusioru ọkọlọtọ A na ìhè ụbọchị 5 min gbasasịrị mgbe nkwadebe nke nkwusioru ọkọlọtọ A.]
Ihe ngwọta hydrazine sulfate: 10 mg / ml nke hydrazine sulfate na mmiri.Hapụ ka ọ kwụrụ maka awa 4 ruo 6 tupu eji ya.
Ngwọta Methenamine: Nyefee 2.5 g nke methenamine na iko 100-mL, tinye 25.0 ml mmiri, fanye ihe nkwụsị iko, ma gwakọta ka ọ gbazee.
Nkwusioru opalescent nke mbụ: Nyefee 25.0 ml nke Hydrazine sulfate ngwọta na 25.0 ml nke Methenamine ngwọta n'ime iko 100-mL kwụsịrị.Gwakọta, ma kwe ka ị kwụrụ maka awa 24.[Rịba ama-Nkwụsịtụ a kwụsiri ike ruo ọnwa 2, ma ọ bụrụhaala na echekwara ya n'ime akpa iko enweghị ntụpọ n'elu.Nkwusioru ahụ ekwesịghị ịrapara na iko a ga-agwakọta nke ọma tupu ejiri ya.]
Ọkọlọtọ opalescence: Jiri mmiri gbanye 15.0 ml nke nkwusioru opalescent nke mbụ na mmiri ruo 1000 ml.[Rịba ama-Ekwesighi iji nkwusioru a gafere awa 24 ka emechara ya.]
Ọkọlọtọ nkwusioru A: Jiri mmiri gbanye 5.0 ml nke ọkọlọtọ Opalescence ka ọ bụrụ 100 ml.
Ọkọlọtọ nkwusioru B: Gwaa 10.0 ml nke ọkọlọtọ Opalescence na mmiri ruo 100 ml.
Ihe ngwọta nlele: 200 mg/mL nke Citric Acid Anhydrous na mmiri
Nyocha
Ọmụmaatụ: Ọkọlọtọ nkwusioru A, Ọkọlọtọ nkwusioru B, mmiri na ihe nlele nlele
Nyefee akụkụ zuru oke nke ihe ngwọta Sample na tube ule nke enweghị agba, transperent, iko na-anọpụ iche na ntọala dị larịị na dayameta dị n'ime nke 15-25 mm iji nweta omimi nke 40 mm.N'otu aka ahụ nyefe akụkụ nke Standard nkwusioru A, Standard nkwusioru B , na mmiri iji kewaa dakọtara ule tubes.Tulee ihe ngwọta Sample, Ọkọlọtọ nkwusioru A, Ọkọlọtọ nkwusioru B, na mmiri dị n'ìhè ụbọchị agbasasịworo, na-ekiri kwụ ọtọ megide ndabere ojii (lee Spectrophotometry na Light-Scattering 851, Ntụle Anya).[Rịba ama-Mgbasa nke ìhè ga-abụrịrị nke a ga-enwe ike ịmata ọdịiche dị n'etiti nkwusioru A na mmiri, yana nkwusioru ọkọlọtọ B nwere ike ịdị iche na nkwusioru ọkọlọtọ A.]
Usoro nnabata: Ihe ngwọta Sample na-egosi otu idoanya dị ka nke mmiri.
• Agba nke Ngwọta
Ọkọlọtọ ngwaahịa ngwọta A: Ferric chloride CS, cobaltous chloride CS, na itughari hydrochloric acid (10 g/L) (2.4:0.6:7.0)
Ọkọlọtọ ngwaahịa ngwọta B: Ferric chloride CS, cobaltous chloride CS, cupric sulfate CS, na itughari hydrochloric acid (10 g/L) (2.4:1.0:0.4:6.2)
Ọkọlọtọ ngwaahịa ngwọta C: Ferric chloride CS, cobaltous chloride CS, na cupric sulfate CS (9.6:0.2:0.2)
[Rịba ama- Kwadebe ihe ngwọta ọkọlọtọ ozugbo tupu ejiri ya.]
Ọkọlọtọ ngwọta A: Gwakọta 2.5 ml nke Standard ngwaahịa ngwọta A na itucha hydrochloric acid (10 g/L) ka 100 ml.
Ọkọlọtọ ngwọta B: Gwakọta 2.5 ml nke Standard ngwaahịa ngwọta B na itughari hydrochloric acid ka (10 g/L) 100 ml.
Ọkọlọtọ ngwọta C: Gwakọta 0.75 ml nke ọkọlọtọ ngwaahịa ngwaahịa C na mbelata hydrochloric acid (10 g/L) ruo 100 ml.
Ngwọta nlere: Jiri ngwọta nlele akwadoro dị ka eduzi ya na nnwale maka Ngwọta doro anya.
Nyocha
Ọmụmaatụ: Ọkọlọtọ ngwọta A, Ọkọlọtọ ngwọta B, Ọkọlọtọ ngwọta C, mmiri, na Sample ngwọta
Nyefee akụkụ zuru oke nke ihe ngwọta Sample na tube ule nke enweghị agba, transperent, iko na-anọpụ iche na ntọala dị larịị na dayameta dị n'ime nke 15-25 mm iji nweta omimi nke 40 mm.N'otu aka ahụ nyefee akụkụ nke Standard Solution A, Standard solution B, Standard solution C, na mmiri iji kewaa tubes ule dabara adaba.Tulee ihe ngwọta Sample, Ọkọlọtọ ngwọta A, Ọkọlọtọ ngwọta B, Ọkọlọtọ ngwọta C, na mmiri dị n'ìhè ụbọchị gbasasịrị, na-elele anya megide ndabere ọcha (lee Spectrophotometry na Light-Scattering 851, Ntụle Anya).
Usoro nnabata: Ngwọta nlele adịghị agba agba karịa ọkọlọtọ ọkọlọtọ A, B, ma ọ bụ C, ma ọ bụ mmiri.
• Ngwa ngwa Carbonizable ihe
Ihe atụ: 1.0 g nke powdered Anhydrous citric acid
Nyocha: Nyefee ihe nlele ahụ na tube ule 22- × 175-mm nke ejiri 10 mL nke sulfuric acid kpochapụ ya na mbụ ma hapụ ya ka ọ gbanye maka 10 min.Tinye 10 mL nke sulfuric acid, kpalie ruo mgbe ngwọta zuru ezu, na-emikpu n'ime mmiri ịsa ahụ na 90 ± 1 maka 60 ± 0.5 min, na-edebe ọkwa nke acid n'okpuru ọkwa mmiri n'oge dum.Mee ka tube ahụ dị jụụ na mmiri na-agba ọsọ, ma bufee acid na tube ntụnyere agba.
Usoro nnabata: Agba nke acid adịghị agba ọchịchịrị karịa nke olu yiri nke Matching Fluid K (lee Agba na Achromicity 631) n'ime tube dabara adaba, a na-ahụ tubes ndị ahụ kwụ ọtọ megide ndabere ọcha.
• Nnwale nke ịmụ nwa 71: Ebe akara ahụ kwuru na Anhydrous Citric Acid adịghị mma, ọ na-emezu ihe achọrọ maka ule Sterility 71 n'ụdị monograph(s) dị mkpa nke ejiri Anhydrous Citric Acid mee ihe.
• Mkpebi mmiri, Usoro I 921: NMT 1.0%
NKWUO GBASARA YA
• Nkwakọ ngwaahịa na Nchekwa: Chekwaa n'ime akpa siri ike.Enweghị ihe nchekwa chọrọ akọwapụtara.
• Ịde aha: Ebe e bu n'obi kee ya na ngwọta dialysis, a na-akpọ ya.Ebe Anhydrous Citric Acid ga-enwerịrị nhazi ọzọ n'oge a na-akwadebe ụdị ọgwụgwọ injectable iji hụ na ọkwa endotoxins nke nje na-anabata nke ọma, a na-akpọ ya aha.Ebe Anhydrous Citric Acid na-adịghị ike, a na-akpọ ya.
• Ụkpụrụ ntụaka USP 11
USP Citric Acid RS Pịa ka ilele Ọdịdị
USP Endotoxin RS