β-Cyclodextrin (β-CD) CAS 7585-39-9 Assay 98.0% ~ 102.0% Ụlọ ọrụ

Ntughari pụrụ iche
Were ngwaahịa a, tụọ ya nke ọma, tinye mmiri ka ọ gbazee na quantitatively itule ya n'ime ngwọta nwere ihe dị ka 10 mg kwa 1 ml, wee chọpụta ya dịka iwu siri dị (Iwu n'ozuzu 0621), ntụgharị a kapịrị ọnụ bụ 159 ° ruo 164 °.
Nchọpụta dị iche iche
Were ihe dị ka 0.2g nke ngwaahịa a, tinye 2ml nke ngwọta nyocha nke iodine, kpoo ya na mmiri ịsa mmiri ka ọ gbazee, ma hapụ ya ka ọ dị jụụ ka ọ pụta ìhè na-acha odo odo-agba aja aja.
N'ime chromatogram nke edere n'okpuru ihe nbibi nke ọdịnaya, oge njigide nke isi ihe kachasị elu nke ngwọta ule kwesịrị ịdị na-adabere na oge njide nke isi ihe ngwọta nchịkwa.6
Ụdị nnabata infrared nke ngwaahịa a kwesịrị ịdị na nke ngwaahịa ntụaka (Iwu n'ozuzu 0402).
Mwepu nke adịghị ọcha
Were ngwaahịa a banyere 1 g, nkenke ịdị arọ, tinye mmiri 100ml ka igbari, dị ka UV-visible spectrophotometry (General rule 0401) mkpebi siri ike, nnabata na nso nso nke 230 ~ 350nm ekwesịghị gafere 0.10, nnabata na nso nso nke wavelength. nke 350 ~ 750nm ekwesịghị gafere 0.05.
pH
were 0.20g nke ngwaahịa a, tinye 20ml mmiri ka igbari, tinye 0.2ml nke juputara potassium chloride ngwọta, na-ekpebi dị ka iwu (General ọchịchị 0631), pH uru kwesịrị 5.0 ~ 8.0.
O doro anya na agba nke ngwọta
Were ngwaahịa a 0. 50g, tinye 50ml mmiri ka ọ gbazee, lelee dịka iwu si dị (Iwu 0901 na iwu izugbe 0902), ngwọta kwesịrị ịdị ọcha na enweghị agba;N'ihe banyere turbidity, jiri ya tụnyere No. 2 Turbidity standard solution (General rule 0902, first method), ghara itinye uche.
Chloride
Were ngwaahịa a 0.39G, nyochara dịka iwu siri dị (Iwu n'ozuzu 0801), na ọkọlọtọ sodium chloride ngwọta 7.0ml ekwesịghị itinye uche karịa ngwọta njikwa (0.018%).
Na-ebelata shuga
Were ngwaahịa a 1.0 g, nkenke nha, tinye mmiri 25ml ka igbari, Tinye alkaline ọla kọpa tartrate ngwọta 40tnl, jiri nwayọọ nwayọọ na-esi nri maka 3 nkeji, tinye na ụlọ okpomọkụ n'otu ntabi anya, iyo na 4 # vetikal agbaze funnel, a na-asacha mmiri ọkụ na mmiri ọkụ. ruo mgbe ngwọta ịsa ahụ na-anọpụ iche.A tụfuru ihe nzacha na ihe ịsa ahụ, ebe ọ na-ekpo ọkụ na ngwọta titration potassium permanganate (0.0M ol/L).Dị ka akọrọ ngwaahịa, oriri nke potassium permanganate titrant (0.02 Mol / l) kwa L g ekwesịghị gafere 3.2ml (1.0%).
Cyclohexane
Were ihe dị ka 0.2g nke ngwaahịa a, na-atụle nkenke, na karama dị elu, gbakwunye ihe ngwọta dị n'ime (were ezigbo dichlorethylene, tinye 20% dimethyl sulfoxide ngwọta iji mee ngwọta nwere ihe dịka 0.04 na lm l ọ bụla, (Njikere dị njikere). 10.0ml, dị ka ule ngwọta; Ọzọ nkenke na-atụ cyclohexane, gbakwunyere esịtidem ọkọlọtọ ngwọta na-eme banyere 1 l / l nwere cyclohexane 0.078mg nke ngwọta, tụọ 10.0ml na a top efu karama dị ka a akara ngwọta. Mkpebi ihe mgbaze fọdụrụnụ (Iwu n'ozuzu 0861), kọlụm capillary nwere 100% dimethylpolysiloxane dị ka mmiri na-adịgide adịgide ka a na-eji kọlụm chromatographic; The kọlụm okpomọkụ bụ 90 ℃; The mbata okpomọkụ bụ 200 ℃; The detector okpomọkụ bụ 250 ℃; na Headspace karama equilibration okpomọkụ bụ 70 ℃ na equilibration oge bụ nkeji 20. Were ntụaka ngwọta n'ime headspace, nkewa ogo n'etiti ọnụ ọnụ nke ọ bụla akụrụngwa kwesịrị dị na ndokwa.A na-agbanye ihe ngwọta ule na ntụnye ntụaka n'otu n'otu n'ime oghere isi, a na-edekọ chromatogram ahụ, a na-agbakọ ebe kachasị elu dịka usoro ọkọlọtọ dị n'ime.
Ọnwụ na ihicha
Were ngwaahịa a, kpọọ nkụ ka ọ dị arọ mgbe niile na 105 ℃, oke ibu agaghị agafe 14.0% (Iwu n'ozuzu 0831).
Ihe fọdụrụ mgbanye
A ga-ewere 1.0 g nke ngwaahịa a maka nyocha dịka iwu siri dị (Iwu n'ozuzu 0841), na ihe fọdụrụ agaghị agafe.
Ọla dị arọ
Nke fọdụrụ n'okpuru ihe nke iwere ihe fọdụrụ mgbanye agaghị enwe ihe karịrị akụkụ 10 kwa nde ígwè dị arọ mgbe iwu nyochara ya (General Principles 0821, Law II).
Oke microbial
A ga-ewere ma nyochaa ngwaahịa a dịka iwu siri dị (General Principles 1105 na 1106).Ngụkọta ọnụ ọgụgụ nke nje aerobic kwa lg nke ngwaahịa ule agaghị agafe 100cfu, ngụkọta ọnụ ọgụgụ nke ebu na yist agaghị agafe 100cfu, E. Coli ekwesịghị ịchọta.
Mkpebi ọdịnaya
Ejiri chromatography mmiri dị elu tụọ ya (General 0512).
Ọnọdụ Chromatographic na nlebara anya nke sistemu eji enyocha silica gel iri na asatọ alkyl silane dị ka ihe ndochi;A na-eji mmiri-methanol (85: 15) mee ihe dị ka usoro mkpanaka;A na-ejikwa ihe nchọta ihe nrịbama dị iche iche mee nha.Ọnụọgụ efere usoro iwu erughị 1500 gbakọọ dị ka ọnụ ọgụgụ betal cyclodextrin.
usoro mkpebi siri ike: were ihe dị ka 50mg nke ngwaahịa a, tụọ ya nke ọma, tinye ya na flask 10ml, tinye mmiri kwesịrị ekwesị ka ọ gbazee ma gbanye ya na ọnụ ọgụgụ, maa jijiji nke ọma, ma jiri ya mee ihe dị ka ihe nyocha, 10/xl. etinyere ya n'ime chromatograph mmiri mmiri nke ọma, ma dekọọ chromatogram ahụ.A tụrụ 50mg ọzọ nke ihe ntụnye aka nke betaloc cyclodextrin nke ọma, a na-ejikwa otu usoro ahụ mee mkpebi.Dị ka usoro ọkọlọtọ mpụga si gbakọọ ebe kachasị elu, ya bụ.