D-(+) -Cycloserine CAS 68-41-7 Assay ≥ 900μg/mg Ụlọ ọrụ dị elu

Cycloserine [68-41-7].
Cycloserine nwere ike na-erughị 900µg nke C3H6N2O2 kwa mg.
Nkwakọ ngwaahịa na nchekwa - Chekwaa n'ime akpa siri ike.
Ụkpụrụ ntụaka USP <11>-
USP Cycloserine RS
Nchọpụta-gbari ihe dịka 1 mg na 10 ml nke 0.1 N sodium hydroxide.Na 1 ml nke ihe ngwọta na-esi na ya pụta tinye 3 ml nke 1 N acetic acid na 1 ml nke ngwakọta, kwadebere 1 hour tupu ejiri ya, nke hà nhata nke sodium nitroprusside solution (1 na 25) na 4 N sodium hydroxide: agba anụnụ anụnụ nke nwayọọ nwayọọ. amalite.
Ngwaahịa condensation-Ahụhụ ya (lee Spectrophotometry na Light-Scattering <851>) na 285 nm, kpebisiri ike na ngwọta 0.1 N sodium hydroxide nwere 0.40 mg kwa mL abụghị ihe karịrị 0.80.
Ntụgharị a kapịrị ọnụ <781S>: n'etiti 108° na 114°.Ngwọta ule: 50 mg kwa ml, na 2 N sodium hydroxide.
Crystallinity <695>: na-emezu ihe achọrọ
pH <791>: n'etiti 5.5 na 6.5, na ngwọta (1 na 10).
Ọnwụ na ihicha <731>-Kọ ihe dịka 100 mg na karama capillar y-stoppered na oghere na 60 ℃ maka awa 3: ọ na-efunahụ ihe karịrị 1.0% nke ibu ya.
Ihe fọdụrụ na mgbanye <281>: ọ bụghị ihe karịrị 0.5%, a na-eji 2 ml nke nitric acid na 5 tụlee nke sulfuric acid mee ihe fọdụrụnụ.
Nyocha -
pH 6.8 Phosphate buffer- Kwadebe dị ka eduzi ya na Ngwọta Buffer n'okpuru Ngwọta na ngalaba Reagents, egosi, na Ngwọta.
Usoro mkpanaka-gbari 0.5 g nke sodium 1-decanesulfonate na mmiri 800 ml, tinye 50 ml nke acetonitrile na 5 ml nke glacial acetic acid, na mix.Gbanwee na 1 N sodium hydroxide ka ọ bụrụ pH nke 4.4.Iyo, na degas.Mee mgbanwe ma ọ bụrụ na ọ dị mkpa y (lee Sistemu dabara n'okpuru Chromatography <621>).
Nkwadebe ọkọlọtọ-N'ụzọ zuru ezu gbarie ọnụọgụ USP Cycloserine RS nke ọma na pH 6.8 Phosphate nchekwa iji nweta ihe ngwọta nwere njiri mara nke ihe dịka 0.4 mg kwa ml.
Nkwadebe nyocha- Nyefee ihe dị ka 20 mg nke Cycloserine, nke a tụrụ nke ọma, gaa na flask volumetric 50-mL, gbazee ma gwakọta ya na pH 6.8 Phosphate buffer na olu, na mix.
Usoro Chromatographic (lee Chromatography <621>) -Kromatograph mmiri mmiri nwere ihe nchọta 219-nm yana kọlụm 4.6mm × 25-cm nke nwere 5-µm mbukota L1.Ọnụego mmiri ahụ bụ ihe dịka 1 ml kwa nkeji.A na-edobe okpomoku kọlụm na ihe dịka 30 Celsius.Chromatograph the Standard nkwadebe, na dekọọ nzaghachi kacha elu dị ka a na-eduzi maka Usoro: ihe ọdụdụ adịghị karịa 1.8;na ndị ikwu ọkọlọtọ ndiiche maka oyiri injections abụghị ihe karịrị 2.0%.
Usoro-iche iche, gbanye nha nha nha (ihe dịka 10 µL) nke nkwadebe ọkọlọtọ na nkwadebe Assay n'ime chromatograph, dekọọ chromatogram, wee tụọ nzaghachi kacha elu maka cycloserine.Gbakọọ ọnụọgụ, na µg, nke C3H6N2O2 n'ime mg nke Cycloserine nke ọ bụla ejiri usoro a:
50,000 (C/W)(rU/rS)
nke C bụ ntinye uche, na mg kwa ml, nke USP Cycloserine RS na nkwadebe Standard;W bụ ọnụọgụ, na mg, nke Cycloserine ewepụtara iji kwado nkwadebe nke Assay;na rU na rS bụ nzaghachi kachasị elu maka cycloserine enwetara site na nkwadebe Assay na nkwadebe Standard, n'otu n'otu.