D-Histidine CAS 351-50-8 HD-His-OH Assay 98.5 ~ 101.0% Ụlọ ọrụ

pH
were 1.0g nke ngwaahịa a, tinye 50ml mmiri ka ọ gbazee, ma tụọ ya dịka iwu siri dị (Iwu n'ozuzu 0631).Uru pH kwesịrị ịbụ 7.0 ~ 8.5.
Mbufe nke Ngwọta
Were 0.60g nke ngwaahịa a, tinye mmiri 20ml ka igbari, dị ka UV-visible spectrophotometry (General rule 0401), tụọ nnyefe na 430nm wavelength, ọ bụghị ihe na-erughị 98.0%.
Chloride
Were 0.25g nke ngwaahịa a wee lelee ya dịka iwu siri dị (Iwu izugbe 0801).E jiri ya tụnyere ngwọta njikwa nke 0.02% nke ọkọlọtọ sodium chloride ngwọta, ọ gaghị etinyekwu uche ().
Sulfate
Were 1.0g nke ngwaahịa a wee lelee ya dịka iwu siri dị (Iwu izugbe 0802).E jiri ya tụnyere ngwọta njikwa nke 0.02% nke ngwọta potassium sulfate ọkọlọtọ, ekwesighi itinye uche karịa ().
Ammonium nnu
were 0.10g nke ngwaahịa a wee lelee ya dịka iwu siri dị (Iwu n'ozuzu 0808).E jiri ya tụnyere ngwọta nchịkwa nke 0.02% nke ọkọlọtọ ammonium chloride ngwọta, ọ gaghị adị omimi ().
Amino acid ndị ọzọ
were ego kwesịrị ekwesị nke ngwaahịa a, tinye mmiri ka ọ gbazee na itughari iji mee ihe ngwọta nwere ihe dị ka 10 mg kwa lml dị ka ngwọta ule;Were 1ml maka nha nkenke ma tinye ya n'ime efere nha 200ml, gbanye ya na mmiri, maa jijiji nke ọma, dịka ngwọta nchịkwa;Were ego kwesịrị ekwesị nke akwụkwọ ntụaka histidine na proline n'otu n'otu, ma tinye n'otu flask ihe atụ, a na-agbakwunye mmiri ka ọ gbazee na diluted iji kwadebe ngwọta nwere ihe dị ka 0.4mg nke ọ bụla kwa 1ml dị ka usoro kwesịrị ekwesị ngwọta.Dị ka mkpa oyi akwa chromatography (General 0502) ule, sere 5 u1 nke ọ bụla n'ime atọ ndị a ngwọta, otu n'otu n'otu na-atụ na otu silica gel G mkpa oyi akwa efere, na N-propanol-concentrated amonia ngwọta (67:33) dị ka na-emepụta ihe mgbaze, gbasaa, nkụ, fesa ninhydrin na ngwọta acetone (1-50), okpomọkụ na 80 Celsius ruo mgbe ntụpọ pụtara, wee nyochaa ozugbo.Ngwọta nchịkwa kwesịrị igosi ntụpọ doro anya, na usoro ọgwụgwọ ọdabara kwesịrị igosi ntụpọ abụọ kewapụrụ kpamkpam.Ọ bụrụ na ngwọta ule na-egosi ntụpọ adịghị ọcha, agba ekwesịghị ịdị omimi (0.5%) karịa isi ntụpọ nke ngwọta nchịkwa.
Ọnwụ na ihicha
were ngwaahịa a, kpoo na 105 ℃ maka awa 3, ọnwụ nke ịdị arọ agaghị agafe 0.2% (Iwu n'ozuzu 0831).
Ihe fọdụrụ na mgbanye
Ọ bụghị karịa 0.1% (Iwu n'ozuzu 0841).
Nnu Ígwè
were 1.0g nke ngwaahịa a wee lelee ya dịka iwu siri dị (Iwu izugbe 0807).E jiri ya tụnyere ngwọta nchịkwa nke 0.001% nke ngwọta ígwè ọkọlọtọ, ọ gaghị adị omimi ().
Ọla dị arọ
Ngwaahịa a 1.0g, nyocha dịka iwu siri dị (General Principles 0821 Iwu mbụ), nwere ọla dị arọ agaghị agafe akụkụ 10 kwa nde.
Nnu Arsenic
were 2.0g nke ngwaahịa a, tinye 23ml mmiri ka igbari, tinye 5ml nke hydrochloric acid, lelee dị ka iwu (General iwu 0822 mbụ iwu), kwesịrị ịgbaso ndokwa (0.0001%).
Endotoxin nke nje bacteria
Were ngwaahịa a, lelee dịka iwu siri dị (Izugbe iwu 1143), lg histidine ọ bụla nwere endotoxin kwesịrị ịbụ ihe na-erughị 6.0EU.(Maka injection)
351-50-8 - Mkpebi ọdịnaya
Were ngwaahịa a ihe dị ka 0.15g, ịdị arọ ziri ezi, gbakwunye anhydrous formic acid 2ml ka ọ gbazee, tinye glacial acetic acid 50ml, dị ka usoro titration nwere ike si dị (Iwu n'ozuzu 0701), na perchloric acid titration solution (0.1 mol/L) titration, na Ejiri ule efu dezie nsonaazụ nke titration.Ihe ngwọta titration perchloric acid 1 ml ọ bụla (0.1 mol/L) dabara na 15.52mg nke C6H9N302.