D-Leucine CAS 328-38-1 HD-Leu-OH Assay 98.5~101.5% Ụlọ ọrụ dị elu

pH
Were 0.50g nke ngwaahịa a, tinye 50ml mmiri, okpomọkụ igbari, jụụ, na-ekpebi dị ka iwu (General 0631), pH uru kwesịrị 5.5 ~ 6.5.
Mbufe nke Ngwọta
Were 0.50g nke ngwaahịa a, tinye 50ml mmiri, okpomọkụ igbari, dị jụụ, ultraviolet-visible spectrophotometry (General rule 0401), tụọ nnyefe na 430nm wavelength, ọ bụghị ihe na-erughị 98.0%.
Chloride
Were 0.25g nke ngwaahịa a wee lelee ya dịka iwu siri dị (Iwu izugbe 0801).E jiri ya tụnyere ngwọta nchịkwa mere nke 5.0 ml nke ọkọlọtọ sodium chloride ngwọta, ọ gaghị etinye uche karịa (0.02%).
Sulfate
Were 1.0g nke ngwaahịa a wee lelee ya dịka iwu siri dị (Iwu izugbe 0802).E jiri ya tụnyere ngwọta njikwa nke 2.0 ml nke ọkọlọtọ potassium sulfate ngwọta, ọ gaghị etinyekwu uche karịa (0.02%).
Ammonium nnu
Were ngwaahịa a 0.10g, lelee dịka iwu siri dị (Iwu n'ozuzu 0808), ma jiri ya tụnyere ngwọta nchịkwa nke ọkọlọtọ ammonium chloride ngwọta 2.0 ML, ọ bụghị omimi (0.02%).
Amino acid ndị ọzọ
Were ego kwesịrị ekwesị nke ngwaahịa a, gbakwunye mmiri ka ọ gbazee na itughari iji mee ihe ngwọta nwere ihe dị ka 20mg kwa lml dị ka ngwọta ule;Were 1ml maka nha nkenke ma tinye ya n'ime efere nha 200ml, gbanye ya na mmiri, maa jijiji, dịka ngwọta nchịkwa;Were ọnụ ọgụgụ kwesịrị ekwesị nke akwụkwọ leucine na ntụ ntụ valine, ma tinye ya n'otu ite ahụ, a na-agbakwunye mmiri ka ọ gbazee ma gbanye ya iji kwadebe ngwọta nwere ihe dị ka 0.4mg nke ọ bụla kwa 1ml dị ka usoro kwesịrị ekwesị ngwọta.Dị ka mkpa oyi akwa chromatography (General 0502) ule, banye n'elu atọ ngwọta ọ bụla 5 u1, karị, na otu silica gel G mkpa oyi akwa efere, na n-butanol-water-glacial acetic acid (3:1:1). maka mmepe, mgbe etinyere ya, ihicha ikuku, fesa ninhydrin na ngwọta acetone (1-50), kpo oku na 80 Celsius ruo mgbe ntụpọ pụtara, na nyocha ozugbo.Ngwọta nchịkwa kwesịrị igosi ntụpọ doro anya, na usoro ọgwụgwọ ọdabara kwesịrị igosi ntụpọ abụọ kewapụrụ kpamkpam.Ọ bụrụ na ngwọta ule na-egosi ntụpọ adịghị ọcha, agba ekwesịghị ịdị omimi (0.5%) karịa isi ntụpọ nke ngwọta nchịkwa.
Ọnwụ na ihicha
Were ngwaahịa a, kpoo na 105 ℃ maka awa 3, ọnwụ nke ịdị arọ agaghị agafe 0.2% (Iwu n'ozuzu 0831).
Ihe fọdụrụ na mgbanye
Were 1.0g nke ngwaahịa a wee lelee ya dịka iwu siri dị (Iwu izugbe 0841).Nke fọdụrụ agaghị agafe 0.1%.
Nnu Ígwè
Were 1.5g nke ngwaahịa a wee lelee ya dịka iwu siri dị (Iwu izugbe 0807).E jiri ya tụnyere ngwọta nchịkwa nke 0.001% nke ngwọta ígwè ọkọlọtọ, ọ gaghị adị omimi ().
Ọla dị arọ
Nke fọdụrụ n'okpuru ihe nke iwere ihe fọdụrụ mgbanye agaghị enwe ihe karịrị akụkụ 10 kwa nde ígwè dị arọ mgbe iwu nyochara ya (General Principles 0821, Law II).
Nnu Arsenic
Tinye mmiri 5ml, 1ml sulfuric acid na 10ml sulfurous acid, kpoo na olu nke ihe dịka 2ml n'ime mmiri ịsa mmiri, tinye mmiri 5ml, tinye Dropwise amonia ngwọta ruo mgbe ihe ngosi nke phenolphthalein na-anọpụ iche, tinye hydrochloric acid 5ml, tinye mmiri iji mee 28ml. , dị ka nyocha iwu (General Principles 0822 Iwu mbụ), kwesịrị ịgbaso ndokwa (0.0001%).
Endotoxin nke nje bacteria
Were ngwaahịa a, lelee dịka iwu siri dị (General 1143), lg leucine ọ bụla nwere endotoxin kwesịrị ịbụ ihe na-erughị 25EU.(Maka injection)
328-38-1 - Mkpebi ọdịnaya
Were ngwaahịa a banyere 0.1g, nkenke ịdị arọ, tinye anhydrous formic acid 1 ml etisasịwo, tinye glacial acetic acid 25ml, dị ka potentiometric titration usoro (General ọchịchị 0701), na perchloric acid titration ngwọta (O. 1 mol/L) titration. , ma jiri ule efu dezie nsonaazụ nke titration.Nke ọ bụla 1 ml nke ngwọta titration perchloric acid (0.1 mol/L) dabara na 13.12 mg nke C6H13N02.