D-(+)-Xylose CAS 58-86-6 Ịdị Ọcha>99.5% (HPLC) Ụlọ ọrụ dị elu

Xylose nwere ihe na-erughị 98.0 pasent na ọ bụghị ihe karịrị 102.0 pasent nke C5H10O5, gbakọọ na akpọnwụwo ndabere.
Nkwakọ ngwaahịa na nchekwa - Chekwaa n'ime akpa siri ike na okpomọkụ ụlọ a na-achịkwa.
Ụkpụrụ ntụaka USP <11>-
USP Fructose RS
USP Xylose RS
Agba nke ngwọta-Ngwọta akwadoro ọhụrụ (1 n'ime 10) doro anya na enweghị agba.
njirimara-
A: Infrared absorption <197A>.
B: Oge njide nke nnukwu ọnụ ọgụgụ dị elu na chromatogram nke nkwadebe Assay kwekọrọ na nke ahụ na chromatogram nke nkwadebe Standard, dị ka enwetara na Assay.
Ntụgharị a kapịrị ọnụ <781S>: n'etiti +18.2° na +19.4°.
Ngwọta ule: 100 mg kwa ml, na 0.012 N ammonium hydroxide.
Ọnwụ na ihicha <731>-Akọrọ 2 g na 5 g na nrụgide na-erughị 50 mm nke mercury na 60 ° na ịdị arọ mgbe nile, ikuku nke ikuku a mịrị amị na-agafe na oven n'oge oge ihicha iji wepụ mmiri vapor: ọ na-efunahụ. ọ bụghị ihe karịrị 0.1% nke ibu ya.
Ihe fọdụrụ na mgbanye <281>: ọ bụghị karịa 0.05%.
Iron <241> - gbazee 2.0 g na mmiri 45 ml, tinye 2 ml nke hydrochloric acid: oke bụ 5 ppm.
Ọla dị arọ <231> - gbazee 2.0 g na mmiri iji mee 25 ml nke ngwọta: njedebe bụ 0.001%.
Chromatographic ịdị ọcha-Jiri chromatogram nke nkwadebe Assay, enwetara dị ka enyere ya na nyocha.Gbakọọ pasent nke adịghị ọcha nke onye ọ bụla, ewezuga ọnụ ọnụ ihe mgbaze ọ bụla, na akụkụ Xylose were:
100 (ri / rS)
nke ri bụ nzaghachi nke onye ọ bụla adịghị ọcha;na rS bụ nchikota nke nzaghachi niile dị na chromatogram: ọ bụghị ihe karịrị 1.0% nke adịghị ọcha onye ọ bụla ka a na-ahụ, na nchikota nke adịghị ọcha nke onye ọ bụla achọtara abụghị ihe karịrị 2.0%.
Nyocha -
Usoro mkpanaaka-Jiri ngwakọta ikuku nke acetonitrile na mmiri (75:25).
Ngwọta dabara adaba sistemu- Kwadebe ngwọta na usoro mkpanaka, nwere ihe dịka 10 mg nke USP Xylose RS na 0.2 mg nke USP Fructose RS kwa ml.
Nkwadebe ọkọlọtọ-Kwụsa USP Xylose RS nke a tụrụ n'ụzọ ziri ezi n'ime usoro mkpanaaka iji nweta ngwọta nwere njiri mara nke ihe dịka 10 mg kwa ml.
Nkwadebe nyocha-Bugharịa ihe dịka 100 mg nke Xylose, atụnyere nke ọma, gaa na flask volumetric 10-mL, gbazee ma gbanye ya na usoro mkpanaka gaa na olu, ma gwakọta ya.
Usoro Chromatographic (lee Chromatography <621>) -Kromatograph mmiri mmiri nwere ihe nchọta 192-nm yana kọlụm 4.6-mm × 15-cm nke nwere nkwakọba L8.A na-edobe okpomoku kọlụm na 30º, na ọnụego eruba bụ ihe dịka 2 ml kwa nkeji.Chromatograph nke dabara adaba sistemu, wee dekọọ nzaghachi kacha elu dị ka akwadoro maka Usoro: mkpebi, R, n'etiti xylose na fructose kacha erughị 2.0.Ọkọlọtọ ọkọlọtọ maka ntụtụ oyiri abụghị ihe karịrị 2.0%.
Usoro-iche iche nha nha (ihe dị ka 25 µL) nke nkwadebe Assay na nkwado ọkọlọtọ n'ime chromatograph, dekọọ chromatogram, wee tụọ nzaghachi maka ọnụ ọgụgụ ndị bụ isi.Gbakọọ ọnụọgụ, na mg, nke C5H10O6, na akụkụ Xylose nke usoro a were were:
10C (rU / rS)
nke C bụ ntinye uche, na mg kwa ml, nke USP Xylose RS na nkwadebe ọkọlọtọ;na rU na rS bụ nzaghachi kacha elu enwetara site na nkwadebe Assay na nkwado ọkọlọtọ, n'otu n'otu.