EDC·HCl CAS 25952-53-8 Njikọta Reagent Dị Ọcha> Ụlọ ọrụ 99.0% (T)

1- (3-Dimethylaminopropyl) -3-Ethylcarbodiimide Hydrochloride (EDC · HCl) (CAS: 25952-53-8) bụ ọcha crystalline ntụ ntụ, mfe deliquescent, soluble na mmiri, soluble na ethanol, eji dị ka onye na-arụ ọrụ reagent maka carboxyl Otu dị na njikọ amide, nke a na-ejikwa maka ịgbalite otu phosphate, njikọ njikọ nke protein na acid nucleic, na mmepụta nke immunocouplers.A na-eji EDC-HCl mee ihe na pH nke 4.0-6.0 ma na-ejikarị ya na N-Hydroxysuccinimide (NHS) ma ọ bụ N-Hydroxysulfosuccinimide sodium nnu iji melite arụmọrụ njikọ.
EDC · HCl bụ mmiri-soluble carbodiimide, nke a na-ejikarị eme ihe maka njikọ peptide.A na-eji EDC·HCl mee ihe dị ka onye na-arụ ọrụ carboxyl.Ọ na-arụ ọrụ dị mkpa maka ikwalite nnukwu biomolecules na njikọ N-hydroxysuccinimide.A na-ejikwa ya na acylation nke phosphoranes.
Mwepụta nke carbodiimide na-agbaze mmiri bara uru maka ijikọta protein na polypeptides.A na-eji emezigharị ndị na-anabata NMDA yana dị ka ihe na-eme ka condensing na njikọ peptide.Isi uru dị na njikọta EDAC bụ mfe iwepụ reagent ngafe yana urea kwekọrọ site na iji acid ma ọ bụ mmiri sachaa.Carbodiimides na-akwalite nguzobe amide bonds, carboxylic acid, na amines site n'ime ka carboxylate rụọ ọrụ iji mepụta O-acylurea.Amine nwere ike ịwakpo etiti a ozugbo ka ọ mepụta amide.A tọhapụrụ EDAC dị ka ihe mgbaze urea soluble.
Enwere ike iji EDC · HCl maka njikọ nke amides.A na-eji ya dị ka onye na-ejikọta ya na njikọ nke esters sitere na acid carboxylic na-eji dimethylaminopyridine dị ka ihe na-akpali akpali.
Enwere ihe atọ a na-ejikarị condensing agents: DCC, DIC na EDC·HCl.Ojiji nke ụdị condensing a n'ozuzu na-achọ ka mgbakwunye nke acylation catalysts ma ọ bụ activators, dị ka DMAP, HOBt, wdg. A naghị eji catalyst mee ihe iji gbanwee ya ka ọ bụrụ ester na-arụ ọrụ kwekọrọ ma ọ bụ amide na-arụ ọrụ, a ga-ahazi ya ka ọ bụrụ ngwaahịa urea (Path B).Onye na-eme ka condensation activator: E nwere ụdị ndị a na-ejikarị eme ihe.Ka ọ dị ugbu a, 4-N, N-dimethylpyridine (DMAP) ejiriwo ya mee ihe n'ọtụtụ ebe iji kpata mmeghachi omume acylation dị iche iche.Mgbe ụfọdụ enwere ike iji 4-PPY mee ihe mgbe mmetụta catalytic nke DMAP adịghị mma.Dị ka akwụkwọ ndị dị mkpa si kwuo, ikike nke ike ya dị ihe dị ka otu puku ugboro karịa DMAP.N'ime condensers atọ a na-ejikarị, DCC na DIC dị ọnụ ala.N'ozuzu, a na-eji DCC na DMAP ọnụ.Otu n'ime nnukwu ọghọm nke iji DCC bụ na ngwaahịa ọzọ nke mmeghachi omume, dicyclohexylurea, nwere obere solubility n'ozuzu organic na-adọ ma ọ bụ ubé soluble, n'ihi ya, ọ na-esi ike wepụ ya nke ọma site na ụfọdụ a na-ejikarị dị ọcha ụzọ, recrystallization. chromatography kọlụm, wdg;n'ihi na solubility nke dicyclohexylurea na ether dị ntakịrị karịa ihe mgbaze ndị ọzọ, ya mere, ọgwụgwọ nke ụdị mmeghachi omume n'ozuzu na-ekpochapụ ihe mgbaze mmeghachi omume ma na-agbakwụnye ether, ma na-ehichapụ ọtụtụ dicyclohexylurea tupu ọgwụgwọ ọzọ.N'ihi na diisopropylurea nke DIC mepụtara nwere mma solubility n'ozuzu organic solvents, ọ na-ejikarị na combinatorial kemịkalụ siri-phase synthesis.A na-eji EDCI eme ihe ugbu a na kemistri ọgwụ.Otu n'ime njirimara ya bụ na urea na-emepụta mgbe mmeghachi omume ahụ na-eme ka mmiri na-agbaze ma dị mfe ịsacha.N'ozuzu, EDCI na-ejikọta ya na HOBt (rịba ama: mmeghachi omume a HOBt bụ ihe dị mkpa n'ozuzu ya, ma ọ bụghị ya, ọ nwere ike iduga n'ụdị condensation dị ala).Mgbe ụfọdụ, ọ bụrụ na nkwụsị ọnọdụ nke acid dị ukwuu ma ọ bụ na e nwere otu ndị na-ewepụ electron, mmeghachi omume ahụ ga-anọ na nzọụkwụ ester na-arụ ọrụ (ester na-arụ ọrụ nwere ihe mgbaàmà siri ike ma ọ bụ MS ma ọ bụ LC-MS nwere ike ịhụ ya).