Flurbiprofen CAS 5104-49-4 Assay 99.0 ~ 100.5% (HPLC) ụlọ ọrụ

Flurbiprofen
C15H13FO2 244.27
(±) -2- (2-Fluoro-4-biphenylyl) propionic acid [5104-49-4].
Flurbiprofen nwere ihe na-erughị 99.0 pasent na ọ bụghị ihe karịrị 100.5 pasent nke C15H13FO2, gbakọọ na akpọnwụwo ndabere.
Nkwakọ ngwaahịa na nchekwa - Chekwaa n'ime akpa siri ike.
Ụkpụrụ ntụaka USP <11>-
USP Flurbiprofen RS
Ihe jikọrọ USP Flurbiprofen A RS
2- (4-Biphenylyl) propionic acid.
C15H14O2 226.28
njirimara-
A: Infrared absorption <197K>.
B: ultraviolet absorption 197U-
Ngwọta: 10 μg kwa ml.
Ọkara: 0.1 N sodium hydroxide.
Mwepu kachasị na 247 nm bụ ihe dịka 0.8.
Oke agbaze <741>: n'etiti 114 na 117.
Ọnwụ na ihicha <731>-Kọchaa ya na agụụ na 60 ka ọ dị arọ mgbe niile: ọ na-efunahụ karịa 0,5% nke ibu ya.
Ihe fọdụrụ na mgbanye <281>: ọ bụghị karịa 0.1%.
Ọla dị arọ, Usoro II <231>: 0.001%.
Ngwakọta ndị emetụtara-
Diluent- Kwadebe ngwakọta nke mmiri na acetonitrile (11: 9).
Usoro mkpanaaka - Kwadebe ngwakọta nke mmiri, acetonitrile, na glacial acetic acid (12:7:1).Mee mgbanwe ma ọ bụrụ na ọ dị mkpa (lee Dabara Sistemu n'okpuru Chromatography <621>).
Ọkọlọtọ ngwaahịa ngwọta- Gbarie n'ụzọ ziri ezi nke USP Flurbiprofen Njikọ Njikọ A RS na Diluent iji nweta ngwọta nwere mkpokọta ihe dị ka 50 μg kwa ml.
Ngwọta dabara adaba sistemụ-Pipet 2.0 ml nke ọkọlọtọ ngwaahịa ọkọlọtọ n'ime flask volumetric 10-mL, tinye ihe dịka 20 mg nke USP Flurbiprofen RS, gbanye ya na diluent ka ọ bụrụ olu, wee gwakọta ya.
Ọkọlọtọ ngwọta-Bugharịa 2.0 ml nke ọkọlọtọ ngwaahịa ọkọlọtọ gaa na flask volumetric 10.0-mL, gbanye ya na diluent ka ọ bụrụ olu, ma gwakọta ya.
Ngwọta nwale-Kwadebe ngwọta nke Flurbiprofen na ihe mgbaze nwere 2.0 mg kwa ml.
Sistemu Chromatographic (lee Chromatography <621>) -Kromatograph mmiri mmiri nwere ihe nchọta 254-nm yana kọlụm 3.9-mm × 15-cm nke nwere ngwugwu 4-µm L1.Ọnụego mmiri ahụ bụ ihe dịka 1 ml kwa nkeji.Chromatograph nke dabara adaba sistemu, wee dekọọ nzaghachi kacha elu dị ka akwadoro maka Usoro: oge ​​njide ikwu ihe dị ka 0.9 maka ngwakọta metụtara Flurbiprofen A na 1.0 maka flurbiprofen;na ndị ikwu ọkọlọtọ ndiiche maka oyiri injections abụghị ihe karịrị 1.0%.
Usoro-iche iche nha nha nha (ihe dị ka 20 µL) nke ọkọlọtọ ọkọlọtọ na ngwọta Nnwale n'ime chromatograph, dekọọ chromatograms, wee tụọ mpaghara maka ọnụ ọnụ kacha elu.Gbakọọ pasent nke ngwakọta flurbiprofen metụtara A na akụkụ nke Flurbiprofen nke usoro a were were:
100 (CS / CU) (rU / rS)
nke CS bụ ntinye uche, na µg kwa ml, nke USP Flurbiprofen Njikọ Njikọ A RS na ngwọta ọkọlọtọ;CU bụ ntinye uche, na µg kwa ml, nke Flurbiprofen na ngwọta ule;na rU na rS bụ nzaghachi kachasị elu maka ihe metụtara flurbiprofen A nwetara site na ngwọta ule na ngwọta Standard, n'otu n'otu: ọ bụghị ihe karịrị 0.5% nke flurbiprofen metụtara compound A hụrụ.Gbakọọ pasent nke adịghị ọcha ọ bụla na akụkụ nke Flurbiprofen nke usoro a were were:
100 (ri / rs)
n'ime nke ri bụ nzaghachi kachasị elu maka adịghị ọcha ọ bụla enwetara site na ngwọta ule;na rs bụ nchikota nke nzaghachi nke ọnụ ọgụgụ kachasị elu nke enwetara site na ngwọta ule: nchikota nke adịghị ọcha niile abụghị ihe karịrị 1.0%.
Assay-Dissolve banyere 0.5 g nke Flurbiprofen, nke a tụrụ n'ụzọ ziri ezi, na 100 ml mmanya, na-ewepụ ya na 0.1 N sodium hydroxide VS na njedebe phenolphthalein, tinye phenolphthalein TS, na titrate na 0.1 N sodium hydroxide VS na-ada mbà n'obi mbụ. agba pink na-adịgide erughị 30 sekọnd.ML nke ọ bụla nke 0.1 N sodium hydroxide bụ 24.43 mg nke C15H13FO2.