Fmoc-Leu-OH CAS 35661-60-0 N-Fmoc-L-Leucine Dị ọcha> Ụlọ ọrụ 99.0% (HPLC)

Usoro nyocha
1. Ọdịdị
-- Nlele anya
2. Ịdị ọcha (HPLC)
2.1 Ngwa
chromatography mmiri mmiri na-arụ ọrụ dị elu, ihe nchọpụta PDA.
Ntụle nyocha eletrọnịkị
2.2 Reagent
Acetonitrile (ọkwa chromatographic), Trifluoroacetic acid (ọkwa chromatographic)
2.3 Ọnọdụ chromatographic
2.3.1 Ogidi: YMC-ODS-AM, 5μL, 250x4.6 mm
2.3.2 Ogologo nchọta: INC220nm
Onu ogugu: 1.0ml/min
Nlele nha: 10μL (ntụaka)
Ihe ngwọta: acetonitrile
Oge nchịkọta data:25.00min
2.4 Mobile usoro nkwadebe
Mobile frasi A (0.1% trifluoroacetic acid water): banye n'ụzọ ziri ezi 2.0ml trifluoroacetic acid, diluted na mmiri na 2000ml, mix ọma, na degassing;
Mobile frasi B (0.1% acetonitrile trifluoroacetic acid): banye n'ụzọ ziri ezi 2.0ml trifluoroacetic acid, diluted ka 2000ml na acetonitrile, ịgwakọta na degassing;
Oge (nkeji) A% B%
0.00 90 10
13.00 10 90
18.00 10 90
18.01 90 10
23.00 90 10
2.6 Nkwadebe nke ngwọta sample
Tụnye na igbari 0.1g sample na acetonitrile na itughari ka 100ml, maa jijiji nke ọma maka ojiji, ma ọ bụ otu itinye uche.Kwadebe ihe atụ abụọ n'otu aka ahụ.
2.7 Nnwale mkpebi
Nyochaa ihe nlele ahụ dịka usoro nlele ndị a si dị:
Ihe kariri ogwu ogwu efu 1
1 ihe ngwọta nke agịga 1 #
1 ihe ngwọta nke agịga 2 #
2.8 Mgbakọ nsonaazụ
2.8.1 A na-eji usoro nhazi nke mpaghara kacha elu iji gbakọọ ịdị ọcha HPLC site n'iwepụ oghere oghere.
2.8.2 Ntugharị ikwu nke ịdị ọcha nke agịga abụọ agaghị adị karịa 1%
2.8.3 Ọ bụrụ na nsonaazụ nke injections abụọ ahụ na-agbaso ụkpụrụ nnabata, a na-ewere nkezi ịdị ọcha dị ka nsonaazụ ikpeazụ.
3, Ebe mgbaze -- RY-1 ihe mgbaze
4. Ọnwụ na usoro nyocha ihicha
4.1 Ngwa:
Igwe ọkụ ọkụ ọkụ eletrik, nguzozi 1/10,000.
4.2 Usoro:
N'ime karama na-atụ ihe dị larịị nke nwere ịdị arọ mgbe niile yana mkpuchi ọnụ ala na-akpọnwụ, tụọ gram 1 (nke ruru 0.0001 gram) nke nlele.The sample kwesịrị evenly gbasaa na ala nke arọ karama na a ọkpụrụkpụ nke ọ dịghị ihe karịrị 10mm, tinye na a thermostatic electric ihicha oven, nkụ na 105 ~ 110 ℃ maka 3 awa, wee banye n'ime ihicha ụlọ ka ajụkwa ka. ọnụ ụlọ okpomọkụ maka ịtụ.
Mgbakọ: Ọnwụ na ihicha %= (M1-M2) ÷M×100
Ebe: M1: arọ nke sample na karama nha tupu ihicha, gram
M2: ịdị arọ nke sample na karama atụ mgbe akpọchara, na gram
Nna-ukwu: Ịdị arọ, gram
5. Ntughari pụrụ iche
-- Specific ntụgharị ka a tụrụ dị ka GB/T613-1988 si dị
Nkwadebe ihe atụ: 0.5000g sample tụrụ n'ụzọ ziri ezi ma kwaga na karama volumetric 50ml dị ọcha ma kpọọ nkụ, 20ml DMF gbakwunyere, karama ahụ kpuchie ma maa jijiji ka ọ gbazee, wee gbanye ya na DMF.
Nnwale: Gbanwee efu nke gyroscope tupu ule ahụ, wee buo tube ule ahụ na ngwọta nlele, dekọọ akụkụ ntụgharị, wee gbakọọ ntụgharị a kapịrị ọnụ nke nlele ahụ na usoro a.
[a]D20=(r×50) ÷ (L×W)
[а] D20: Ntugharị ngwa anya akọwapụtara na 25 ℃ nke ngwọta nlele
r: Ntugharị anya hụrụ na 20 ℃ maka ngwọta nlele
50: Mpịakọta nke ngwọta sample akwadoro (ml)
w: Nlereanya arọ (g)
L: Ogologo tube ntụgharị anya (dm)