H-Glu(OEt) -OEt·HCl CAS 1118-89-4 L-Glutamic Acid Diethyl Ester Hydrochloride Ụlọ Ọrụ>99.0% (HPLC) Ụlọ ọrụ

I. Usoro nyocha
1. Ebe mgbaze
A na-eji ngwa mgbaze dijitalụ
Nzọụkwụ 2: Ọdịnaya
2.1 Reagent
Nzọụkwụ 1: Formic acid
2. Glacial acetic acid
3. Perchloric acid ọkọlọtọ titration ngwọta (C=0.1000mol/l)
4. Crystal Violet egosi mmiri mmiri: 5g / l.
2.2 Ngwa na akụrụngwa
1. Beaker 150ml
2. Microburet: 10ml
2. 3 Usoro nyocha
2. 3. M n'ụzọ ziri ezi tụọ banyere 300mg nke sample tupu akpọnwụwo na 105 ℃ maka 3h, tinye ya n'ime a 150ml Bethel, tinye 3ml nke formic acid, tinye 50ml nke ice acetic acid, tinye 2 tụlee nke crystal violet ule ngwọta ( TS-74), na titrate na 0.1mol / L perchloric acid ruo mgbe njedebe akwụkwọ ndụ akwụkwọ ndụ ma ọ bụ ruo mgbe acha anụnụ anụnụ ga-apụ kpamkpam.
2. 3. 2 Usoro mgbako
X hà nhata C (V1-V2) ugboro 0.2042 / M ugboro 100
C bụ ntinye nke ngwọta titration ọkọlọtọ perchloric acid, nke egosipụtara na moles kwa liter (mol/l).
V1 bụ olu riri nke perchloric acid ọkọlọtọ titration ngwọta etinyebere na titration sample, nke egosipụtara na ml (ml).
Mpịakọta nke ngwọta titration ọkọlọtọ perchloric acid n'ime ule oghere V2, gosipụtara na ml (ml)
0.2042 na 1.00ml perchloric acid ọkọlọtọ titrated lekwasịrị anya mmiri mmiri mgbe egosipụtara àgwà nke ngwaahịa a na G
M Ogo nke nlele ahụ, gosipụtara na gram (g).
2.4 Ndozi
Ọ bụrụ na ọdịiche okpomọkụ dị n'ime ụlọ dị n'etiti ngwọta ule titration na nhazi ọkọlọtọ perchloric acid karịrị 10 ℃, a ga-emegharị ya ọzọ;Ma ọ bụghị ya, a ga-edozi ntinye uche C1 nke ngwọta ọkọlọtọ perchloric acid dịka ule (2) siri dị.
C2 = C1/1+0.0011 (t1-t2)
1+0.0011 bụ ọnụọgụ mgbasawanye nke glacial acetic acid
Akụkụ nke okpomọkụ ụlọ maka T2 calibration nke perchloric acid bụ Celsius (° C).
Akụkụ nke ụlọ okpomọkụ nke a na-edepụta ihe nlele bụ Celsius (° C).
C1 t2 ℃ perchloric acid ọkọlọtọ ngwọta ịta, na moles kwa liter (MOL / L)
3. Akọrọ na enweghị arọ
3.1 Ngwa:
Igwe ọkụ ọkụ ọkụ, nguzozi 1/10,000.
3.2 Usoro:
Were gram 1-2 nke ihe nlele (nke ziri ezi na 0.5mg) wee tinye ha n'ime karama ntụ ntụ a mịrị amị mgbe niile.Ihe nlele ahụ ga-enwe oke nha na ala nke karama atụ.Banyere ihe atụ, a ga-akpọnwụ ha na oven (105 ± 5 ℃) ọkụ ọkụ na-adị arọ mgbe niile.
3.3 Mgbakọ
X bụ m mwepu m1 kewara site m wepụrụ m2 ugboro 100
Ebe: X- iru mmiri %
m1 -- Ibu nke ihe nlele akọrọ gbakwunyere karama atụ g
m2 - tụọ oke karama g
m- Ibu nke ihe nlele g
3.4 Isi maka Ntị
1. Ihe nlebara anya: 1. A ghaghị ime ihe atụ ndị yiri ya maka ihicha na enweghị arọ.Otu ihe atụ nwere nsonaazụ abụọ, na njehie dị n'etiti nsonaazụ abụọ ahụ abụghị ihe karịrị 0.5%.
2. Ịdị arọ ga-akpachara anya, ziri ezi na 0.0001.
4 na ntụgharị
4.1 Ụkpụrụ
Ìhè nke ụgbọelu na-agafe na mmiri mmiri ma ọ bụ ihe ngwọta nke ogige nwere ụfọdụ ọrụ anya (ya bụ, ihe na-arụ ọrụ anya) nwere ike ime ka ntụgharị anya anya, na-eme ka ụgbọ elu nke ìhè polarized na-atụgharị gaa n'aka ekpe ma ọ bụ aka nri.A na-akpọ ọnụọgụ ogo nke ntụgharị ntụgharị anya.Na a ụfọdụ okpomọkụ (na-egosipụtakarị site t, nwere ike ịbụ 20 ℃ ma ọ bụ 25 ℃) a ụfọdụ wavelength nke ìhè (odo sodium ìhè na D, wavelength λ 589.3nm) polarized ìhè site ọ bụla milliliter nwere 1g ngwa anya ọrụ ihe, ọkpụrụkpụ nke 1dm. (ya bụ, 10cm) ngwọta mgbe a na-akpọ ntụgharị ntụgharị anya kpọmkwem.
4.2 chọrọ
A na-eji ahịrị D nke ụdị sodium dị iche iche (589. 3 nm) tụọ ogologo tube nke 1dm na ọnọdụ okpomọkụ bụ 20 ℃.
4.3 Ngwa
4.3.1 Digital autotropy (nkenke ±0.001°)
4.3.2 Eletrọnịkị itule (nke ziri ezi 0.0001g).
4.4 Usoro ọrụ
Were 3g nke ngwaahịa a, nke ziri ezi na 0.0001g, tinye ya na karama volumetric 100ml, tinye ethanol ka ọ gbazee ma gbanye ya na ọnụ ọgụgụ, maa jijiji nke ọma.Jiri obere mmiri sụọ ihe ahụ ọtụtụ ugboro, wee jiri nwayọọ nwayọọ tinye mmiri ahụ n'ime tube ihe atụ (emela ka afụ pụta), tinye tube n'ime gyroscope maka nchọpụta, wee gụọ akwụkwọ ntụgharị anya maka nyocha. ugboro 3 n'otu n'otu.
4.5 Mgbakọ
A na-agbakọ ntụgharị a kapịrị ọnụ nke L-glutamate Diethyl carbonate na sodium spectrum D-line na 20 ℃ [Alpha] D20 dị ka ndị a:
[ɑ] D20 = alfa/m
Ebe: α -- nkezi ọnụọgụ ọgụgụ cubic ntụgharị;
m -- ihe atụ àgwà.
4.6 Enwere ike ime ihe dị iche: Ọdịiche zuru oke nke otu nlele agaghị agafe 0.02% mgbe a tụrụ ya ugboro abụọ.
4.7 Akpachara anya
4.7.1 Ekwesịrị iji ihe mgbaze mee ihe dị ka nhazi oghere tupu nha ọ bụla: Mgbe nhachara, a ga-eme mmezi ọzọ iji chọpụta ma akara efu na-agbanwe n'oge nha.Ọ bụrụ na enwere mgbanwe ọ bụla, a ga-ekpebi ntụgharị anya anya nke nlele ọzọ.
4.7.2 The sample kwesịrị dokwuo anya mgbe mgbasa na dilution;ma ọ bụghị ya, a ga-enyocha ya.
Nzọụkwụ 5 Ọdịdị
Ngwaahịa ahụ ga-abụ ntụ ntụ microcrystalline na-acha ọcha ma na-acha ọcha