Histamine Dihydrochloride CAS 56-92-8 Assay 98.5 ~ 101.0% (Titration) Factory

Histamine Dihydrochloride (CAS: 56-92-8) EP9.0 Mpịakọta Ⅱ Ụzọ nnwale
Nkọwa
Histamine dihydrochloride nwere ọ bụghị ihe na-erughị pasent 98.5 na ọ bụghị karịa ihe nhata nke 101.0 pasent nke 2- (1H-imidazole-4-yl)ethan-1-amine dihydrochloride, gbakọọ na-ezo aka na ihe a mịrị amị.
ÀMÀ
Acha ọcha ma ọ bụ ihe fọrọ nke nta ka ọ bụrụ ọcha, kristal ntụ ntụ ma ọ bụ kristal enweghị agba, hygroscopic, soluble na mmiri, soluble na mmanya.
Nchọpụta
njirimara nke mbụ: A, D.
Ihe njirimara nke abụọ: B, C, D
A. Nyochaa site na infrared absorption spectrophotometry (2.2.24), iji tụnyere ụdịdị dị iche iche enwetara na histamine dihydrochloride CRS.Nyochaa dị ka diski akwadoro site na iji 1 mg nke ihe.
B. Nyochaa chromatograms enwetara na ule maka histidine.Ebe bụ isi na chromatogram nke enwetara na ngwọta nnwale (b) yiri n'ọnọdụ, agba na nha ya na ebe bụ isi na chromatogram nwetara site na ntụnye ntụaka (a).
C. Gbari 0.1 g na 7 ml mmiri R ma gbakwunye 3 ml nke 200 g / L ngwọta nke sodium hydroxide R. Gbari 50mg nke sulfanilic acid R na ngwakọta nke 0.1 mL nke hydrochloric acid R na 10 ml mmiri R na tinye 0.1 mL nke sodium nitrite solution R. Tinye ihe ngwọta nke abụọ na nke mbụ na mix.A na-emepụta agba uhie.
D. Ọ na-enye mmeghachi omume (a) nke chlorides (2.3.1).
Nnwale
Ngwọta S. Gbari 0.5g n'ime mmiri na-enweghị carbon dioxide R nke a kwadebere site na mmiri distilled R wee gbasaa na 10 ml na otu ihe mgbaze.
Ọdịdị nke ngwọta.Ngwọta S doro anya (2.2.1) na ọ bụghị nke ukwuu agba karịa ntụaka ngwọta Y7 (2.2.2, Usoro II).
pH (2.2.3).pH nke ngwọta S bụ 2.85 ruo 3.60.
Histidine.Nyochaa site na chromatography nke dị obere (2.2.27), na-eji TLC silica gel G plate R.
Ngwọta ule (a).Gbari 0.5g nke ihe a ga-enyocha na mmiri R wee gbanye 10 ml na otu ihe mgbaze.
Ngwọta ule (b).Gwakọta 2 ml nke ngwọta ule (a) ruo 10 ml na mmiri R.
Ngwọta ntụaka (a).Gbari 0.1 g nke histamine dihydrochloride CRS n'ime mmiri R wee gbasaa na 10 ml na otu ihe mgbaze.
Ngwọta ntụaka (b).Gbarie 50 mg nke histidine monohydrochloride R n'ime mmiri R wee jiri otu ihe mgbaze mee ka ọ bụrụ 100 ml.
Ngwọta ntụaka (c).Gwakọta 1 ml nke ngwọta ule (a) na 1 ml nke ngwọta ntụaka (b).
Tinye n'ime efere 1 μL nke ngwọta ule (a), 1 μL nke ngwọta nyocha (b), 1 μL ntụ ntụ ntụ ntụ (a), 1 μL ntụ ntụ ntụ (b) na 2 μL ntụ ntụ ntụ ntụ (c).Mepụta n'elu ụzọ nke 15cm site na iji ngwakọta nke 5volumes nke amonia R na-etinye uche, 20 mpịakọta mmiri R na 75 nke acetonitrile R. Ghichaa efere na ikuku dị ugbu a.Tinyegharịa mmepe ahụ n'otu ụzọ ahụ, kpoo efere ahụ na ikuku ikuku ma fesaa ya na ninhydrin solution R1.Kpoo efere ahụ na 110 ° C maka 10 min.Ebe ọ bụla dabara na histidine na chromatogram enwetara na ngwọta nnwale (a) adịghị ike karịa ebe dị na chromatogram nwetara na ntụzịaka ntụaka (b) (1%).Nnwale ahụ abaghị uru ọ gwụla ma chromatogram enwetara na ntụzịaka ntụaka (c) gosiri ntụpọ abụọ kewapụrụ nke ọma.
Sulfates (2.4.13).3mL nke ngwọta S gbazere na 15 mL na mmiri distilled R na-akwado ule oke maka sulfates (0.1%).
Ọnwụ na ihicha (2.2.32).Ọ bụghị ihe karịrị 0,5 pasent, kpebisiri ike na 0.20 g site na ihicha na oven na 105 Celsius C.
Sulfated ash (2.4.14).Ọ bụghị karịa 0.1 pasent, kpebisiri ike na 0,5 g.
ASSAY
Gbari 0.080 g na ngwakọta nke 5.0 ml nke 0.01 M hydrochloric acid na 50 ml mmanya R. Mepụta titration potentiometric (2.2.20), na-eji 0.1 M sodium hydroxide.Gụọ olu agbakwunyere n'etiti isi ihe mbụ na nke atọ nke mgbanwe.
1mL nke 0.1 M sodium hydroxideis dabara na 9.203 mg nke C5H11Cl2N3.
Nchekwa
Chekwaa n'ime akpa ikuku, nke na-echebe site na ìhè.