L-Cysteine ​​​​CAS 52-90-4 (H-Cys-OH) Nyocha 98.5 ~ 101.0% (Titration) Ụlọ ọrụ dị elu

L-Cysteine ​​​​nwere ihe na-erughị 98.5% na ọ bụghị karịa 101.0% nke L-Cysteine ​​​​(C3H7NO2S), gbakọọ na ntọala a mịrị amị.
Nkọwa L-Cysteine ​​​​dị ka kristal ọcha ma ọ bụ ntụ ntụ ọcha kristal.Ọ nwere ihe e ji mara isi na ụtọ pungent.Ọ na-agbaze n'efu n'ime mmiri, yana ọ fọrọ nke nta ka ọ ghara ịsacha ya na ethanol (99.5).Ọ na-agbaze na 1 mol/L hydrochloric acid TS.
Kpebie ụdị nnabata infrared nke L-Cysteine ​​​​dị ka akwadoro na usoro potassium bromide disk n'okpuru Infrared Spectrophotometry <2.25>, wee tụle ụdịdị dị iche iche na Spectrum Reference: spectra abụọ ahụ na-egosipụta ike nnabata ahụ n'otu ọnụọgụ ebili mmiri.
Ntugharị anya <2.49> [a] 20D: + 8.0 ~ + 10.0° (2 g gbakọrọ na ntọala a mịrị amị, 1 mol/L hydrochloric acid TS, 25mL, 100 mm).
pH <2.54> pH nke ngwọta a kwadebere site na igbari 1.25 g nke L-Cysteine ​​​​na 50 ml mmiri bụ 4.7 ruo 5.7.
Ịdị ọcha (1) Ịdị ọcha na agba nke ngwọta-Gbasa 1.0 g nke L-Cysteine ​​​​na 20 ml mmiri: ngwọta doro anya na enweghị agba.
(2) Chloride <1.03> - gbazee 0.30 g nke L-Cysteine ​​​​na 10mL nke nitric acid diluted (1 n'ime 4), tinye 10ml hydrogenperoxide (30), kpoo maka nkeji 20 n'ime mmiri esi mmiri saa, dị jụụ, wee gbakwunye mmiri na-eme ka 50 ml.Mee ule ahụ site na iji ngwọta a dị ka ngwọta ule.Kwadebe ngwọta nchịkwa na 0.35 ml nke 0.01 mol / L hydrochloric acid VS (ọ bụghị karịa 0.041%).
(3) Sulfate <1.14> - Gbanyụọ 0.6 g nke L-Cysteine ​​​​na 30 ml mmiri na 3 mL nke dilute hydrochloric acid, ma tinye mmiri iji mee 50 ml.Mee ule ahụ site na iji ngwọta a dị ka ngwọta ule.Kwadebe ngwọta nchịkwa dị ka ndị a: Iji 0.35 ml nke 0.005 mol / L sulfuric acid VS tinye 3 mL nke dilute hydrochloric acid na mmiri iji mee 50 ml.Kwadebe ngwọta ule na ngwọta nchịkwa na 4 ml nke barium chloride TS, n'otu n'otu (ọ bụghị karịa 0.028%).
(4) Ammonium <1.02>-Mee ule ahụ na 0.25 g nke L-Cysteine ​​​​, na-eji distillation n'okpuru nrụgide belata.Kwadebe ngwọta nchịkwa na 5.0 ml nke Standard Ammonium Solution (ọ bụghị karịa 0.02%).
(5) Ọla dị arọ <1.07>-Gaa na 1.0g nke L-Cysteine ​​​​dị ka usoro 4 si dị, ma mee ule ahụ. Kwadebe ngwọta nchịkwa na 1.0 mL nke Standard Lead Solution (ọ bụghị karịa 10 ppm).
(6) Iron <1.10> - Kwadebe ngwọta ule na 1.0 g nke L-Cysteine ​​​​dị ka usoro 1 si dị, ma mee ule ahụ dịka usoro A. Kwadebe ngwọta nchịkwa na 1.0mL nke Standard Iron Solution (ọ bụghị karịa 10 ppm). ).
(7) Ihe ndị metụtara ya - gbazee 0.10 g L-Cysteine ​​​​na ngwọta N-ethylmaleimide (1 n'ime 50) iji mee kpọmkwem 10 ml, hapụ maka nkeji 30, ma jiri ngwọta a dị ka ngwọta nlele.Pipet 1 mL nke ihe nlele, gbakwunye mmiri iji mee kpọmkwem 10 ml, pipet 1 mL nke ngwọta a, gbakwunye mmiri iji mee kpọmkwem 50 ml, ma jiri ngwọta a dị ka ọkọlọtọ ọkọlọtọ (1).Iche iche, igbari 0.10 g nke L-cystine na 0.5mol/L hydrochloric acid TS mee kpọmkwem 20 ml.Pipet 1mL nke ihe ngwọta a, gbakwunye mmiri iji mee 100 ml, ma jiri ngwọta a dị ka ọkọlọtọ ọkọlọtọ (2).Jiri ngwọta ndị a mee nnwale ahụ dị ka a gwara gị n'okpuru Chromatography nke Thin-Layer<2.03>.Kpatụ 10mL nke ọ bụla n'ime ihe nlele nlele na ngwọta ọkọlọtọ (1) na (2) na efere silica gel maka chromatography nke dị nro.Mepụta efere ahụ na ngwakọta nke 1-butanol, mmiri, na acetic acid (100) (3: 1: 1) ruo ihe dị ka 10 cm, wee kpoo efere ahụ maka nkeji 30 na 809C. fesaa efere ahụ nke ọma na ngwọta. nke ninhydrin na ngwakọta nke methanol na acetic acid (100) (97: 3) (1 na 100), wee kpoo ọkụ na 80 ℃ maka nkeji 10: ntụpọ enwetara site na ngwọta nlele kwekọrọ na ntụpọ enwetara site na ọkọlọtọ ọkọlọtọ. (2) adịghị ike karịa ntụpọ sitere na ngwọta ọkọlọtọ (2).Ọzọkwa, ntụpọ ndị ọzọ na-abụghị isi ntụpọ na ntụpọ ndị a kpọtụrụ aha n'elu site na ngwọta nlele adịghị ike karịa ntụpọ sitere na ngwọta ọkọlọtọ (1).
Ọnwụ na ihicha <2.41> Ọ bụghị ihe karịrị 0.50% (1 g, na oghere, phosphorus (V) oxide, awa 3).
Ihe fọdụrụ na mgbanye <2.44> Ọ bụghị karịa 0.1% (1 g).
Nyochaa nha nke ọma banyere 0.2 g nke L-Cysteine ​​​​, tinye ya n'ime flask kwụsịrị, ma gbazee n'ime 20 ml mmiri.Gbari 4 g nke potassium iodide na ngwọta a, tinye ozugbo na mmiri oyi oyi, tinye 5 ml nke dilute hydrochloric acid na kpọmkwem 25 ml nke 0.05 mol / L iodine VS, hapụ n'ebe gbara ọchịchịrị maka nkeji 20, wee titrate <2.50 > ngafe ego nke ayodiin nwere 0.1 mol/L sodium thiosulfate VS (ihe ngosi: starch TS).Mee mkpebi efu site na iji otu usoro ahụ.
ML nke ọ bụla nke 0.05 mol/L ayodiin VS = 12.12 mg nke C3H7NO2S
Ebube na ihe nchekwa - akpa ndị siri ike.