Levodopa (L-DOPA) CAS 59-92-7 99.0 ~ 100.5% USP BP EP Standard Anti-Parkinson's Disease High Purity

Ntụgharị anya [EP]
Gbarie ihe dị ka 0.200g nke ihe a kpọrọ nkụ na 5g nke hexamethylenetetramine R n'ime 10ml nke 1mol/L hydrochloric acid wee gbanye 25.oml n'otu acid ahụ.Kwe ka ihe ngwọta guzoro na nchekwa ọkụ maka awa 3.Nkuku nke ntụgharị anya bụ -1.27° ruo -1.34°
Ọdịdị nke Ngwọta
Wepu 1.0g n'ime 1mol/L hydrochloric acid wee jiri otu solvnet tụgharịa ka ọ bụrụ 25ml.Ihe ngwọta adịghị agba agba karịa ntụaka ntụaka BY6.
Ihe ndị emetụtara [EP]
Nyochaa site na chromatography nke dị obere, jiri cellulose maka chromatography R dị ka ihe mkpuchi.
Ngwọta ule-Gbasa 0.1g nke ihe a ga-enyocha na 5ml nke anhydrous formic acid R ma gbanye 10ml na methanol R. Kwadebe ozugbo tupu eji ya.
Ntụaka ngwọta (a) -Gbanye 0.5ml nke ngwọta ule na 100ml na methanol R.
Ngwọta ntụaka (b) - Gbanyụọ 30mg nke tyrosine R na 1ml nke anhydrous formic acid R ma gbanye 100ml na methanol R. Gwakọta 1ml nke ngwọta a na 1ml nke ngwọta ule.
Tinye iche na efere ahụ dị ka eriri 20mm ogologo, 1oμl nke ngwọta ule, 10μl nke ngwọta ntụaka (a) na 20μl nke ngwọta ntụaka (b).Kpọọ n'ime ikuku dị ugbu a.Mepụta n'ụzọ od 15cm site na iji ngwakọta nke 50 mpịakọta butanol R, mpịakọta 25 nke glacial acetic acid R na oke mmiri 25.Ghichaa efere ahụ n'ime ikuku ọkụ ugbu a ma fesaa ya na ngwakọta a kwadebere ọhụrụ nke nha nha nha nke 10% m/v ngwọta nke ferric chloride R na 5% m/v ngwọta nke postassium ferricyanide R. Nyochaa chromatoframs ozugbo.Ebe ọ bụla dị na chromatogram nke enwetara na ngwọta ule, ewezuga ebe bụ isi, adịghị ike karịa ebe dị na chromatogram nke ejiri ngwọta ntụnye aka (a) gosipụtara.Nnwale ahụ abaghị uru ọ gwụla ma chromatogram enwetara na ntụzịaka ntụaka (b) na-egosi, n'elu ebe bụ isi, ebe dị iche nke siri ike karịa ebe dị na chromatogram nwetara site na ngwọta ntụaka (a).
UV Spectrum [EP]
Wepu 30.0mg n'ime 0.1mol/L hydrochloric acid wee jiri otu acid mee ka ọ bụrụ 100.oml.Tinye 10.0ml nke ngwọta a ka ọ bụrụ 100.0ml na 0.1mol/L hydrochloric acid.Nyochaa n'etiti 230nm na 350nm, ngwọta na-egosi otu nnabata kacha na 280nm.Ihe nnabata a kapịrị ọnụ na nke a kachasị bụ 137 ruo 147, gbakọọ na ntụnyere ihe a mịrị amị.
Ọnwụ na ihicha
Were ngwaahịa a, kpọọ nkụ ka ọ dị arọ mgbe niile na 105 ° C, oke ibu agaghị agafe 1.0% (Iwu n'ozuzu 0831).
Ihe fọdụrụ na mgbanye (Sulfated)
Were l.0g nke Levodopa wee lelee ya dịka iwu siri dị (Iwu izugbe 0841).Nke fọdụrụ agaghị agafe 0.1%.
Ọla dị arọ
Nke fọdụrụ n'okpuru ihe nke iwere ihe fọdụrụ mgbanye agaghị enwe ihe karịrị akụkụ 10 kwa nde ígwè dị arọ mgbe iwu nyochara ya (General Principles 0821, Law II).
Nnwale pH
0.10g na 10ml nke H2O na-ama jijiji maka nkeji 15.
Mkpebi ọdịnaya
Were ngwaahịa a banyere 0.lg, nkenke ịdị arọ, tinye anhydrous formic acid 2ml ka igbari, tinye glacial acetic acid 20ml, maa jijiji, tinye crystal violet egosi 2 tụlee, na perchloric acid titration ngwọta (0.1 mol / L) titration na ngwọta bụ. akwụkwọ ndụ akwụkwọ ndụ, a na-edozikwa nsonaazụ nke titration na ule efu.Nke ọ bụla 1 ml nke ngwọta titration perchloric acid (0.1 mol/L) dabara na 19.72mg nke C9H11N04.

Usoro ule JP17

Levodopa, mgbe akpọnwụwo, nwere ihe na-erughị 98.5% nke Levodopa (C9H11NO4).
Nkọwa Levodopa na-apụta dị ka ọcha ma ọ bụ obere isi awọ, kristal ma ọ bụ ntụ ntụ kristal.Ọ dịghị isi.Ọ na-agbaze n'efu na formic acid, na-agbaze ntakịrị na mmiri, yana ihe fọrọ nke nta ka ọ bụrụ ihe a na-apụghị sosolument na ethanol (95).Ọ na-agbaze na dilute hydrochloric acid.PH nke ihe ngwọta zuru oke nke Levodopa dị n'etiti 5.0 na 6.5.Ebe agbaze: banyere 275 ℃ (na ire ere).
njirimara
(1) Na 5 ml nke ihe ngwọta nke Levodopa (1 na 1000) tinye 1mL nke ninhydrin TS, na okpomọkụ maka nkeji 3 n'ime mmiri ịsa ahụ: agba odo na-etolite.
(2) Na 2 ml nke ngwọta nke Levodopa (1 n'ime 5000) tinye 10 ml nke 4-aminoantipyrine TS, wee maa jijiji: agba uhie na-amalite.
(3) igbari 3 mg nke Levodopa na 0.001 mol/L hydrochloric acid TS iji mee 100 ml.Kpebie ụdịdị dị iche iche nke ngwọta dị ka eduzi n'okpuru Ultravioletvisible Spectrophotometry <2.24>, ma tulee ụdịdị dị iche iche na Spectrum Reference: ụdịdị ụdịdị abụọ ahụ na-egosipụta ike nke mmịpụta n'otu ogologo ogologo oge.
Absorbance <2.24> E 1% 1cm (280 nm): 136-146 (mgbe ihichachara, 30 mg, 0.001 mol/L hydrochloric acid TS, 1000 ml).
Ntugharị anya <2.49> [a] 20D: -11.5° ~-13.0°(mgbe ihichachara, 2.5 g, 1 mol/L hydrochloric acid TS, 50 ml, 100 mm).
Ịdị ọcha
(1) Ịdị ọcha na agba nke ngwọta-Gbasa 1.0 g nke Levodopa na 20 ml nke 1 mol / L hydrochloric acid TS: ngwọta doro anya na enweghị agba.
(2) Chloride <1.03>-Gbasa 0.5 g nke Levodopa na 6 mLof dilute nitric acid, ma gbakwunye mmiri iji mee 50 ml.Mee ule ahụ site na iji ngwọta a dị ka ngwọta ule.Kwadebe ngwọta nchịkwa na 0.30 ml nke 0.01 mol/L hydrochloricacid VS (ọ bụghị karịa 0.021%).
(3) Sulfate <1.14> - Gbari 0.40 g nke Levodopa na 1 mL nke dilute hydrochloric acid na 30 ml mmiri, ma gbakwunye mmiri iji mee 50 ml.Mee ule ahụ site na iji ngwọta a dị ka ngwọta ule.Kwadebe ngwọta nchịkwa na 0.25 ml nke 0.005 mol / L sulfuric acid VS (ọ bụghị karịa 0.030%).
(4) Ọla dị arọ <1.07>-Gaa na 1.0 g nke Levodopa dị ka usoro 2 si dị, ma mee ule ahụ.Kwadebe ngwọta njikwa na 2.0 ml nke Standard Lead Solution (ọ bụghị karịa 20 ppm).
(5) Arsenic <1.11> - Gbanyụọ 1.0 g nke Levodopa na 5 mLof dilute hydrochloric acid, ma mee ule ahụ na ngwọta a dị ka ngwọta ule (ọ bụghị karịa 2 ppm).
(6) Ihe ndị metụtara ya - gbazee 0.10 g nke Levodopa na 10 ml nke sodium disulfite TS, ma jiri ngwọta a dị ka ihe ngwọta nlele.Pipet 1 mL nke ngwọta nlele, gbakwunye sodium disulfite TS iji mee kpọmkwem 25 ml.Pipet 1 ml nke ihe ngwọta a, tinye sodium disulfite TS iji mee kpọmkwem 20 ml, ma jiri ngwọta a dị ka ọkọlọtọ ọkọlọtọ.Jiri azịza ndị a mee nnwale dị ka eduzi n'okpuru Chro-matography Thin-layer<2.03>.Kpatụ 5mL nke ọ bụla n'ime ihe nlele nlele yana ngwọta ọkọlọtọ n'elu efere cellulose maka mkpa-layerchromatography.Mepụta efere ahụ na ngwakọta nke 1-butanol, mmiri, acetic acid (100) na methanol (10: 5: 5: 1) ruo ihe dịka 10 cm, wee kpoo efere ahụ.Na-efesa ngwọta nke ninhydrin n'ime acetone (1 n'ime 50) na efere ahụ ma kpoo ọkụ na 90 ℃ maka nkeji 10: ntụpọ ndị ọzọ karịa ebe bụ isi site na ngwọta nlele adịghị ike karịa ebe sitere na ngwọta ọkọlọtọ.
Ọnwụ na ihicha <2.41> Ọ bụghị ihe karịrị 0.30% (1 g, 105 ℃, awa 3).
Ihe fọdụrụ na mgbanye <2.44> Ọ bụghị karịa 0.1% (1 g).
Assay tụọ n'ụzọ ziri ezi banyere 0.3 g nke Levodopa, nke a mịrị amị, gbarie na 3 mL nke formic acid, tinye 80 ml acetic acid (100), na titrate <2.50> na 0.1 mol / L perchloric acid VS ruo mgbe agba nke ngwọta gbanwere. site na-acha odo odo site na-acha anụnụ anụnụ-acha akwụkwọ ndụ na-acha akwụkwọ ndụ (ihe ngosi: 3 tụlee nke crystalviolet TS).Mee mkpebi efu, ma mee mgbazi ọ bụla dị mkpa.
Otu ml nke 0.1 mol/L perchloric acid VS 19.72 mg nke C9H11NO4
Ebube na ihe nchekwa-akpa ndị siri ike.
Nchekwa-Ìhè na-eguzogide.