L-Glutamine CAS 56-85-9 (H-Gln-OH) Nyocha 99.0 ~ 101.0% Ụlọ ọrụ dị elu

Japanese PharmacopoeiaỤkpụrụ ntụaka
L-Glutamine (56-85-9)
L-Glutamine, mgbe akpọnwụwo, nwere ihe na-erughị 99.0% na ọ bụghị karịa 101.0% nke L-Glutamine (C5H10N2O3).
Nkọwa L-Glutamine pụtara dị ka ọcha, kristal ma ọ bụ ntụ ntụ kristal.Ọ nwere ntakịrị ụtọ njirimara
Ọ na-agbaze n'efu na formic acid, soluble na mmiri, yana ọ fọrọ nke nta ka ọ bụrụ ihe a na-adịghị ahụkebe na ethanol (99.5)
Kpebie ụdị nnabata infrared nke L-Glutamine dị ka eduzi ya na usoro potassium bromide disk n'okpuru Infrared Spectrophotometry <2.25>, wee jiri ụdịdị dị iche iche tụnyere Spectrum Reference: spectra abụọ ahụ na-egosipụta ike nke absorption n'otu ọnụọgụ ebili mmiri.
Ntugharị anya <2.49> [a] 20D: + 6.3° ka +7.3° tụọ nke ọma banyere 2 g nke L-Glutamine, nke a mịrị amịburu, tinye 45mL mmiri, kpoo ọkụ ruo 40℃ ka igbari, na mgbe ọ jụrụ, tinye mmiri ka ọ mee. kpọmkwem 50 ml.Kpebie ntụgharị anya nke ngwọta a na cell 100mm, n'ime nkeji 60.
pH <2.54> pH nke ngwọta a kwadebere site na igbari 1.0 g nke L-Glutamine na 50 ml mmiri dị n'etiti 4.5 na 6.0.
Ịdị ọcha
(1) Ịdị ọcha na agba nke ngwọta-Ngwọta nke enwetara site na igbari 0.5 g nke L-Glutamine na 20 ml mmiri doro anya na enweghị agba.
(2) Chloride <1.03> -Jiri 0.5 g nke L-Glutamine mee ule ahụ.Kwadebe ngwọta nchịkwa na 0.30 ml nke 0.01 mol / L hydrochloric acid VS (ọ bụghị karịa 0.021%).
(3) Sulfate <1.14>-Mee ule na 0.6 g nke L-Glutamine.Kwadebe ngwọta nchịkwa na 0.35 ml nke 0.005 mol/L sulfuric acid VS (ọ bụghị karịa 0.028z).
(4) Ammonium <1.02> - Mee ule ahụ na 0.10 g nke L-Glutamine, na-eji distillation n'okpuru nrụgide belatara.Kwadebe ngwọta nchịkwa na 10.0 ml nke Standard Ammonium Solution.Okpomọkụ nke mmiri ịsa ahụ bụ 45 ℃ (ọ bụghị karịa 0.1%)
(5) Ọla dị arọ <1.07> - Gaba na 1.0 g nke L-Glutamine dị ka usoro 1 siri dị, wee mee ule ahụ.Kwadebe ngwọta nchịkwa na 1.0 ml nke Standard Lead Solution (ọ bụghị karịa 10 ppm).
(6) Ígwè <1.10> - Kwadebe ngwọta ule na 1.0 g nke L-Glutamine dị ka usoro 1 si dị, ma mee ule ahụ dịka usoro A. Kwadebe ngwọta nchịkwa na 1.0 ml nke Standard Iron Solution (ọ bụghị karịa 10). ppm)
(7) Ihe ndị metụtara ya - gbazee 0.10 g nke L-Glutaminein 10 ml mmiri, ma jiri ngwọta a dị ka ihe nlele.Pipet 1 mL nke ngwọta nlele, gbakwunye mmiri iji mee kpọmkwem 10 ml.Pipet 1 ml nke ngwọta a, gbakwunye mmiri ka ọ bụrụ kpọmkwem 50 ml, ma jiri ngwọta a dị ka ngwọta ọkọlọtọ.Jiri ngwọta ndị a mee nnwale ahụ dị ka a gwara gị n'okpuru Chromatography nke Thin-Layer <2.03>.Kpatụ 5mL nke ọ bụla n'ime ihe nlele nlele na ngwọta ọkọlọtọ na efere silicagel maka chromatography nke dị obere.Wee mepụta ya na ngwakọta nke 1-butanol, mmiri na acetic acid (100) (3: 1: 1) ruo ihe dị ka 10 cm, wee kpoo efere ahụ na 80 ℃ maka nkeji 30.Fesa ihe ngwọta nke ninhydrin n'otu n'otu na ngwakọta nke methanol na acetic acid (100) (97: 3) (1 n'ime 100) na efere ahụ, wee kpoo ọkụ na 80 ℃ maka nkeji 10: ntụpọ ọzọ na-abụghị isi ntụpọ nwetara na efere. sample ngwọta adịghị ike karịa ntụpọ enwetara na ọkọlọtọ ngwọta.
Ọnwụ na ihicha <2.41> Ọ bụghị ihe karịrị 0.3% (1 g, 105 ℃, awa 3)
Ihe fọdụrụ na mgbanye <2.44> Ọ bụghị karịa 0.10% (1 g).
Assay tụọ n'ụzọ ziri ezi banyere 0.15 g nke L-Glutamine, nke a mịrị amị, gbarie na 3 ml nke formic acid, tinye 50 ml nke acetic acid (100), na titrate <2.50> na 0.1 mol/L perchloric acid VS (potentiometric titration).Mee mkpebi efu n'otu ụzọ ahụ, ma mee mgbazi ọ bụla dị mkpa.
Otu ml nke 0.1 mol/L perchloric acid VS 14.61 mg nke C5H10N2O3
Ebube na ihe nchekwa - akpa ndị siri ike.

Ụkpụrụ ntụaka USP <11>-USP Glutamine RS.
njirimara, Infrared absorption <197K>.
Ntụgharị a kapịrị ọnụ <781S>: n'etiti +6.3° na +7.3°, kpebisiri ike na 20℃
Ngwọta Nleba- Na flask kwesịrị ekwesị, dozie nke ọma ihe ngwọta nke ihe dịka 40mg kwa ml n'ime mmiri n'ihe dịka 40 ℃.Mee ka ọ dị jụụ ma gbanye mmiri ka ọ bụrụ ụda tupu ejiri ya.
Ọnwụ na ihicha <731>-Hichaa ya na 105 ℃ maka awa 3;ọ na-efunahụ ihe karịrị 0.20% nke ibu ya.
Ihe fọdụrụ na mgbanye <281>Ọ bụghị ihe karịrị 0.30%
Chloride <221>- Akụkụ 0.7-g na-egosi enweghị chloride karịa kwekọrọ na 0.50ml nke 0.020 N hydrochloric acid: ọ bụghị ihe karịrị 0.05% ka a hụrụ.
Sulfate <221>- Akụkụ 0.8-g na-egosi na ọ dịghị ihe ọzọ sulfate karịa kwekọrọ na 0.25ml nke 0.020 N sulfuric acid: ọ bụghị ihe karịrị 0.03% ka a hụrụ.
Chromatographic ịdị ọcha
Adsorbent: 0.25-mm oyi akwa nke chromatographic silica gel ngwakọta.
Ngwọta ule: 10mg kwa ml, n'ime mmiri
Ọkọlọtọ Ngwọta- Kwadebe ihe ngwọta nke USP Glutamine RS n'ime mmiri na-enwe mkpokọta amara nke ihe dịka 0.05mg kwa ml.
Ngwa ngwa: 5μL
Na-emepe usoro mgbaze: ngwakọta nke mmanya butyl, mmiri, na glacial acetic acid (3:1:1).
Spray Reagent - gbazee 0.2g nke ninhydrin na 100ml nke ngwakọta nke mmanya butyl na 2 N acetic acid (95:5)
Usoro-Gaa n'ihu dịka akwadoro maka chromatography nke dị obere n'okpuru Chromatography <621>, wee kpochaa efere ahụ na 80 ℃ maka nkeji 30.Gbasaa efere ahụ na reagent ịgba, kpoo ọkụ na 80 ℃ maka nkeji 10, wee nyochaa n'okpuru ìhè ọcha: enweghị ntụpọ nke abụọ na chromatogram enwetara site na ngwọta nnwale nke buru ibu ma ọ bụ dị ike karịa ebe isi na chromatogram nwetara site na ngwọta standrad. (0.5%)
Ihe adịghị ọcha nke Organic, Usoro m <467>: Zute ihe achọrọ.
Assy- Nyefee ihe dị ka 150mg nke Glutamine, nha nke ọma, gaa na 125ml flask, gbazee na 3ml nke formic acid na 50ml nke glacial acetic acid, na titrate na 0.1 N perchloric acid VS na-ekpebi njedebe njedebe nke potentiometrically.Mee mkpebi efu, ma mee mgbazi ọ bụla dị mkpa (lee Titrimetry <541>).Otu ml nke 0.1 N perchloric acid bụ 14.615 mg nke C5H10N2O3.