L-Serine CAS 56-45-1 (H-Ser-OH) Nyocha 98.5 ~ 101.0% Ụlọ ọrụ dị elu

JP16
L-Serine, mgbe akpọnwụwo, nwere ihe na-erughị 98.5% na ọ bụghị karịa 101.0% nke C3H7NO3.
Nkọwa L-Serine pụtara dị ka kristal ọcha ma ọ bụ ntụ ntụ kristal.Ọ nwere ntakịrị ụtọ ụtọ.Ọ na-agbaze n'efu na mmiri yana na formic acid, yana ọ fọrọ nke nta ka ọ ghara ịsacha ya na ethanol (99.5).Ọ na-agbaze na 2 mol / L hydrochloric acid TS.
Kpebie ụdị nnabata infrared nke L-Serine dị ka eduzi ya na usoro potassium bromide disk n'okpuru Infrared Spectrophotometry<2.25>, wee jiri ụdịdị dị iche iche tụnyere Spectrum Reference: spectra abụọ ahụ na-egosipụta ike nke absorption n'otu ọnụọgụ ebili mmiri ahụ.
Ntugharị anya <2.49> [a] 20D: +14.0°~+16.0°(Mgbe akọrọ akọrọ, 2.5 g, 2 mol/L hydrochloric acid TS, 25 ml, 100 mm).
pH <2.54> pH nke ngwọta a kwadebere site na igbari 1.0 g nke L-Serine na 10 ml mmiri dị n'etiti 5.2 na 6.2.
Ịdị ọcha (1) Ịdị ọcha na agba nke ngwọta-Gbasa 1.0 g nke L-Serine na 10 ml mmiri: ngwọta doro anya na enweghị agba.
(2) Chloride <1.03>-Mee ule na 0.5 g nke L-Serine.Kwadebe ngwọta nchịkwa na 0.30 ml nke 0.01mol / L hydrochloric acid VS (ọ bụghị karịa 0.021%).
(3) Sulfate <1.14> Mee ule na 0.6 g nke L-Serine.Kwadebe ngwọta nchịkwa na 0.35 ml nke 0.005mol / L sulfuric acid VS (ọ bụghị karịa 0.028%).
(4) Ammonium <1.02>-Mee ule na 0.25 g nke L-Serine.Kwadebe ngwọta nchịkwa na 5.0 ml nke Standard Ammonium Solution (ọ bụghị karịa 0.02%).
(5) Ọla dị arọ <1.07> Gaba na 2.0 g nke L-Serine dị ka usoro 1 si dị, ma mee ule ahụ.Kwadebe ngwọta nchịkwa na 2.0 ml nke Standard Lead Solution (ọ bụghị karịa 10 ppm).
(6) Ígwè <1.10> Kwadebe ngwọta ule na 1.0 g nke L-Serine dị ka usoro 1 si dị, ma mee ule ahụ dịka usoro A. Kwadebe ngwọta nchịkwa na 1.0mL nke Standard Iron Solution (ọ bụghị karịa 10 ppm) .
(7) Ihe ndị emetụtara-Gbasa 0.10 g nke L-Serine na mmiri 10mL, ma jiri ngwọta a dị ka ngwọta nlele.Pipet 1 mL nke ngwọta nlele, gbakwunye mmiri iji mee kpọmkwem 10 ml.Pipet 1 mL nke ngwọta a, gbakwunye mmiri ka ọ bụrụ kpọmkwem 50 mL, ma jiri ngwọta a dị ka ihe ngwọta ọkọlọtọ. Mee ule na ngwọta ndị a dị ka ntụziaka n'okpuru Thin-layer Chromatography <2.03>.Chọpụta 5mLeach nke ihe nlele na ngwọta ọkọlọtọ na efere silica gel forthin-layer chromatography.Wepụtazie ya na ngwakọta nke 1-butanol, mmiri na acetic acid (100) (3:1:1) n'ebe dị anya ihe dịka 10 cm, wee kpoo efere ahụ na 809C maka nkeji iri atọ.Sprayvenly ngwọta nke ninhydrinin ngwakọta nke methanol andacetic acid (100) (97:3) (1 n'ime 100) na efere, na okpomọkụ na 80 ℃ maka nkeji 10: ntụpọ na-abụghị isi ntụpọ enwetara site sample ngwọta abụghị. ọzọ ufiop thanthe ntụpọ si ọkọlọtọ ngwọta.
Ọnwụ na ihicha <2.41> Ọ bụghị ihe karịrị 0.3% (1 g, 105 ℃, awa 3).
Ihe fọdụrụ na mgbanye <2.44> Ọ bụghị karịa 0.1% (1 g).
Assay tụọ n'ụzọ ziri ezi banyere 0.11 g nke L-Serine, nke a mịrị amị, gbarie na 3 ml nke formic acid, tinye 50 ml nke acetic acid (100), na titrate <2.50> na 0.1 mol/L perchloric acid VS (potentiometric titration).Mee mkpebi efu n'otu ụzọ ahụ, ma mee mgbazi ọ bụla dị mkpa.
Otu ml nke 0.1 mol/L perchloric acid VS 10.51 mg nke C3H7NO3
Ebube na ihe nchekwa-akpa ndị siri ike.

USP39-NF34
Nkọwa L-Serine nwere NLT 98.5% na NMT 101.5% nke L-serine (C3H7NO3), gbakọọ na ntọala akpọnwụwo.
Nchọpụta
A. AKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWASỊ"197K>
ASSAY
Usoro
Ihe atụ: 100mg nke serine
Oghere: Gwakọta 3ml nke formic acid na 50ml nke glacial acetic acid.
Titrimetric usoro
(Lee Titrimetry <541>)
Ụdị: Direct titration
Titrant: 0.1 N perchloric acid VS
Nchọpụta njedebe: Potentiometric
Nyocha: gbazee ihe nlele ahụ na 3ml nke formic acid na 50ml nke glacial acetic acid.Titrate na Titrant.
Mee mkpebi nke oghere ahụ.
Gbakọọ pasentị nke serine (C3H7NO3) n'omume ewepụtara:
Nsonaazụ = {[(VS-VB) xNxF]/W} x100
VS= olu titrant nke Sample (mL) na-eri.
VB = Olu titrant nke oghere (mL) na-eri.
N = ezigbo usoro nke Titrant (mEq/ml)
F= ihe nhata, 105.1mg/mEq
W= Nlereanya arọ (mg)
Usoro nnabata: 98.5% ~ 101.5% na ntọala akpọnwụwo
IHE adịghị ọcha
MKWAGHỊ ihe fọdụrụ <281>: NMT 0.1%
ChlorideAND sulfate, chloride <221>
Ọkọlọtọ ngwọta: 0.50mL nke 0.020 N hydrochloric acid
Ihe atụ: 0.73g nke Serine
Usoro nnabata: NMT 0.05%
ChlorideAND sulfate, sulfate <221>
Ihe ngwọta ọkọlọtọ: 0.10ml nke 0.020 N sulfuric acid
Ihe atụ: 0.33g nke Serine
Usoro nnabata: NMT 0.03%
IRON <241>: NMT 30ppm
Hichapụ ihe ndị a:
Ọla dị arọ, Usoro I <231>: NMT 15ppm (Ọchịchị 1-Jan-201)
Ngwakọta ejikọtara
Ngwọta ndabara sistemu: 0.4mg/mL nke ọ bụla nke USP L-Serine RS na USP L-Methionine RS na 0.1 N hydrochloric acid
Ngwọta ọkọlọtọ: 0.05mg/mL nke USP L-Serine RS na 0.1 N hydrochloric acid.[IHE-Ngwọta a nwere ntinye uche ya na ihe dịka 0.5% nke ngwọta Sample.]
Ngwọta nlele: 10mg/mL nke Serine na 0.1 N hydrochloric acid
Sistemu chromatographic Hụ Chromatography <621>, Chromatography nke dị obere.)
Ụdị: TLC
Adsorbent: 0.25-mm oyi akwa nke chromatographic silica gel ngwakọta
Olu ngwa: 5μL
Sistemụ ihe mgbaze na-emepe emepe: mmanya butyl, mmanya glacial acebutyl na 2N acetic acid (95:5)
Sistemu kwesịrị ekwesị
Ihe achọrọ dabara adaba: chromatogram nke ngwọta dabara adaba sistemụ na-egosipụta ntụpọ abụọ kewapụrụ nke ọma.
Nyocha
Nlereanya: Ngwọta dabara adaba sistemu, Ngwọta ọkọlọtọ, na ngwọta nlele.
Mgbe ịsachara efere ahụ ikuku, fesaa ya na reagent Spray, wee kpoo ọkụ n'etiti 100 ° na 105 ° maka 15 min.Nyochaa efere n'okpuru ọkụ ọcha.
Usoro nnabata: Ebe ọ bụla nke abụọ nke ngwọta Sample adịghị ibu ma ọ bụ sie ike karịa isi ntụpọ nke Standard Solution.
Ihe adịghị ọcha n'otu n'otu: NMT 0.5%
Mkpokọta adịghị ọcha: NMT 2.0%
Nnwale akọwapụtara
Ntụgharị anya, ntụgharị a kapịrị ọnụ <781S>
Ngwọta nlele: 100mg/mL na 2N hydrochloric acid
Ụkpụrụ nnabata: +14.0° ruo +15.6°
ỌKỤKWU KWESỊRỊ <731>: Kpochapụ ihe nlele na 105 ℃ maka awa 3: ọ na-efunahụ NMT 0.2% nke ịdị arọ ya.
NKWUO GBASARA YA
NKWAKWỌKWỌ KWESỊRỊ: Chekwaa n'ime akpa mechiri emechi nke ọma.
Ụkpụrụ ntụaka USP <11>
USP L-Methionine RS
USP L-Serine RS