L-Tryptophan CAS 73-22-3 (H-Trp-OH) Nyocha 98.5 ~ 101.5% Ụlọ ọrụ dị elu

L-Tryptophan (H-Trp-OH) (CAS: 73-22-3) Usoro ule AJI 97
Nchọpụta: Tụlee ụdị nnabata infrared nke ihe nlele ahụ na nke ọkọlọtọ site na usoro diski bromide potassium.
Ntụgharị a kapịrị ọnụ [α]20/D: Nlereanya akpọnwụwo, C=1, H2O gbarie site na ikpo ọkụ
Ọnọdụ Ngwọta (Nfefe): 0.5g na 20ml nke 2 mol/L HCl, spectrophotometer, 430nm, 10mm cell ọkpụrụkpụ.
Chloride (Cl): 0.7g, A-1, ntụgharị: 0.40ml nke 0.01mol/L HCl
Ammonium (NH4): B-2
Sulfate (SO4): 1.2g, (1), ntụgharị: 0.50ml nke 0.005mol/L H2SO4
Ígwè (Fe): 0.75g, (2), ntụgharị: 1.5ml nke Ígwè Std.(0.01mg/ml)
Ọla dị arọ (Pb): 2.0g, (4), ntụgharị: 2.0ml nke Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (1), ntụgharị: 2.0ml nke As2O3 Std.
Amino acids ndị ọzọ: H2O, Gbarie site na ikpo ọkụ (40 ℃) Nlele ule: 50μg, A-6-a, njikwa: L-Trp 0.25μg
Ọnwụ na ihicha: na 105 ℃ maka awa 3.
Ihe fọdụrụ na mgbanye (Sulfated): AJI Test 13
Nyocha: Mịrị amị sample, 200mg, (1), 3ml nke formic acid, 50ml nke glacial acetic acid, 0.1mol/L HCLO4 1ml=20.423mg C11H12N2O2
Nnwale pH: 1.0g na 100ml nke H2O

L-Tryptophan (H-Trp-OH) (CAS: 73-22-3) Uzo ule USP35
Nkọwa
Tryptophan nwere NLT 98.5% na NMT 101.5% nke C11H12N2O2, dị ka L-Tryptophan, gbakọọ na ntọala akpọnwụwo.
Nchọpụta
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ASSAY
Usoro
Ngwọta nlele: Tinye 200 mg nke Tryptophan n'ime flask 125-mL.Gbarie na ngwakọta nke 3 ml nke formic acid na 50 ml nke glacial acetic acid.
Nyocha: Titrate na 0.1 N perchloric acid VS, na-ekpebi ebe njedebe nwere ike.Mee mkpebi efu, ma mee mgbazi ọ bụla dị mkpa (lee Titrimetry <541>).Otu ml nke 0.1 N perchloric acid bụ 20.42 mg nke C11H12N2O2.
Usoro nnabata: 98.5% ~ 101.5% na ntọala akpọnwụwo
IHE adịghị ọcha
Ihe adịghị ọcha
MKWAGHỊ ihe fọdụrụ <281>: NMT 0.1%
CHLORIDE AND SULFATE, Chloride <221>: Akụkụ 0.73-g na-egosi na ọ nweghị chloride karịa kwekọrọ na 0.50 ml nke 0.020 N hydrochloric acid (0.05%).[IHE-Jiri nwayọọ kpoo nkwadebe nlele ka ọ gbazee, ọ bụrụ na ọ dị mkpa.]
Chloride na sulfate, Sulfate <221>: Akụkụ 0.33-g na-egosi na ọ dịghị ihe ọzọ sulfate karịa kwekọrọ na 0.10 ml nke 0.020 N sulfuric acid (0.03%).[IHE-Jio nwayọọ kpoo nkwadebe nlele ka ọ gbazee, ọ bụrụ na ọ dị mkpa.
IRON <241>: NMT 30ppm
Ọla dị arọ, Usoro II <231>: NMT 15ppm
Ihe adịghị ọcha
Usoro 1
Ngwọta A: Trifluoroacetic acid na mmiri (1 mL / L)
Ngwọta B: Trifluoroacetic acid na acetonitrile na mmiri ngwọta (80:20) (1 mL / L trifluoroacetic acid ngwọta)
Ọkọlọtọ ngwọta: 1.0 mg / L nke ọ bụla nke USP Tryptophan metụtara Compound A RS na USP Tryptophan metụtara Compound B RS na mmiri.
Ihe ngwọta nlele: 10.0 mg / ml nke tryptophan n'ime mmiri
Ngwọta ndabara sistemu: 1.0 mg/L nke USP Tryptophan metụtara Compound B RS n'ime mmiri
Usoro mkpanaka: Lee tebụl gradient n'okpuru.

Sistemu Chromatographic (Lee Chromatography <621>, Ndabara Sistemu.)
Ụdị: LC
Ihe nchọpụta: UV 220 nm
Ogidi: 4.6-mm × 25-cm;5-µm mbukota L1
Okpomọkụ kọlụm: 30°
Ọnụego mmiri: 1 ml / min
Nha igba ogwu: 20µL
Sistemu kwesịrị ekwesị
Ọmụma: Ngwọta dabara adaba sistemụ
Kwesịrị ekwesị chọrọ
Ngbanwe ọkọlọtọ emetụtara: NMT 5.0%
Nyocha
Ihe atụ: Ngwọta ọkọlọtọ na ngwọta nlele
Gbakọọ pasent nke adịghị ọcha ọ bụla akọwapụtaghị n'akụkụ nke Tryptophan were:
Nsonaazụ = (rU/rS) × (CS/CU) × 100
rU = mpaghara kacha elu nke adịghị ọcha ọ bụla na-akọwapụtaghị na ngwọta Sample
rS = mpaghara kacha elu nke ngwakọta tryptophan metụtara B na ngwọta ọkọlọtọ
CS = ntinye uche nke USP Tryptophan metụtara Compound B RS na ngwọta ọkọlọtọ (µg/mL)
CU = itinye uche nke Tryptophan na ngwọta nlele (µg/mL)
Usoro nnabata
Mkpokọta adịghị ọcha 1: NMT 0.01% nke mkpokọta adịghị ọcha na-apụta tupu elu tryptophan.
Mkpokọta adịghị ọcha 2: NMT 0.03% nke mkpokọta adịghị ọcha na-apụ apụ mgbe elu tryptophan gasịrị.[MARA-wepu ọnụ ọgụgụ kasị elu maka ngwakọta metụtara tryptophan B.]
Ngwakọta metụtara Tryptophan A: Ọ bụrụ na a na-ahụ ọnụ ọgụgụ kasị elu maka ihe metụtara tryptophan A na ngwọta Sample, wee mee nnwale maka Usoro 2: Oke nke Compound metụtara Tryptophan A, n'okpuru.
Usoro 2: OKWU KWUKWU MMETỤTA TRYPTOPHAN A
Ngwọta A: 18 mM monobasic sodium phosphate, nzacha na degassed (pH 2.5), na acetonitrile (9: 1)
Ngwọta B: 10 mM monobasic sodium phosphate, nzacha na degassed (pH 2.5), na acetonitrile (1: 1)
Ngwọta C: Acetonitrile n'ime mmiri (7: 3)
Ọkọlọtọ ngwọta: 0.1 mg/L nke USP Tryptophan metụtara Compound A RS na mmiri
Ihe ngwọta nlele: 10.0 mg / ml nke Tryptophan n'ime mmiri
Usoro mkpanaka: Lee tebụl gradient n'okpuru

Sistemụ chromatographic
(Lee Chromatography <621>, Kwesịrị ekwesị Sistemu.)
Ụdị: LC
Ihe nchọpụta: UV 216 nm
Ogidi: 3.9-mm × 15-cm;5-µm mbukota L1
Okpomọkụ kọlụm: 30°
Ọnụego mmiri: 1 ml / min
Nha igba ogwu: 20µL
Sistemu kwesịrị ekwesị
Ihe atụ: Ngwọta ọkọlọtọ
Kwesịrị ekwesị chọrọ
Ngbanwe ọkọlọtọ emetụtara: NMT 5.0%
Nyocha
Ihe atụ: Ngwọta ọkọlọtọ na ngwọta nlele
Gbakọọ pasent nke ngwakọta tryptophan metụtara
A na akụkụ nke Tryptophan were:
Nsonaazụ = (rU/rS) × (CS/CU) × 100
rU = mpaghara kacha elu nke ngwakọta tryptophan metụtara A na ngwọta Sample
rS = mpaghara kacha elu nke ngwakọta tryptophan metụtara A na ngwọta ọkọlọtọ
CS = ntinye uche nke USP Tryptophan metụtara Compound A RS na ngwọta ọkọlọtọ (µg/mL)
CU = itinye uche nke Tryptophan na ngwọta nlele (µg/mL)
Usoro nnabata: NMT 10 ppm
Nnwale akọwapụtara
Ntụgharị anya, Mgbanwe a kapịrị ọnụ <781S>: -29.4° ruo -32.8°
Ihe ngwọta nlele: 10 mg / ml, n'ime mmiri.[MARA- Kwuo nwayọọ ka ọ gbazee, ọ bụrụ na ọ dị mkpa.]
PH <791>: 5.5 ~ 7.0, na ngwọta (1 n'ime 100)
Ọnwụ na nkụ <731>: Kpochapụ ihe nlele na 105 ° maka awa 3: ọ na-efunahụ NMT 0.3% nke ibu ya.
NKWUO GBASARA YA
Nkwakọ ngwaahịa na nchekwa: Chekwaa n'ime akpa mechiri emechi nke ọma
Ụkpụrụ ntụaka USP <11>
USP L-Tryptophan RS
Ngwakọta jikọtara USP Tryptophan A RS
3,3′-[Ethylidenebis (1H-indole-1,3-diyl)]bis[2S) -2-aminopropanoic] acid.C24H26N4O4 432.49
Ngwakọta jikọtara USP Tryptophan B RS
2-Acetamido-3- (1H-indol-3-yl) propanoic acid.C13H14N2O3 246.3