L-(-)-Tyrosine CAS 60-18-4 (H-Tyr-OH) Nyocha 98.5 ~ 101.5% Ụlọ ọrụ dị elu

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) AJI 97 Test Method
Nchọpụta: Tụlee ụdị nnabata infrared nke ihe nlele ahụ na nke ọkọlọtọ site na usoro diski bromide potassium.
Ntụgharị a kapịrị ọnụ [α]20/D: Nlereanya akpọnwụwo, C=5, 1mol/L HCl
Ọnọdụ Ngwọta (Nfefe): 0.5g na 10ml nke 1mol/L HCl, spectrophotometer, 430nm, 10mm cell ọkpụrụkpụ.
Chloride (Cl): 0.7g, A-1, ntụgharị: 0.40ml nke 0.01mol/L HCl
Ammonium (NH4): B-2
Sulfate (SO4): 1.2g, (1), ntụgharị: 0.50ml nke 0.005mol/L H2SO4
Ígwè (Fe): 1.5g, (2), ntụgharị: 1.5ml nke Ígwè Std.(0.01mg/ml)
Ọla dị arọ (Pb): 2.0g, (2), ntụgharị: 2.0ml nke Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (2), ntụgharị: 2.0ml nke As2O3 Std.
Amino acid ndị ọzọ: Dilute amonia R2 Nlele ule: 50μg, F-6-a, njikwa: L-Tyr 0.25μg
Ọnwụ na ihicha: na 105 ℃ maka awa 3.
Ihe fọdụrụ na mgbanye (Sulfated): AJI Test 13
Nyocha: Mịrị amị sample, 180mg, (1), 6ml nke formic acid, 50ml nke glacial acetic acid, 0.1mol/L HCLO4 1ml=18.119mg C9H11NO3
Nnwale pH: 0.02g na 100ml ngwọta mmiri juru

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) Ule ule USP35
Nkọwa
Tyrosine nwere NLT 98.5% na NMT 101.5% nke L-Tyrosine (C9H11NO3), gbakọọ na ntọala akpọnwụwo.
Nchọpụta
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ASSAY
Usoro
Ihe atụ: 180 mg nke Tyrosine
Oghere: Mix 6 ml nke formic acid na 50 ml nke glacial acetic acid.
Titrimetric usoro
(Lee Titrimetry <541>)
Ụdị: Direct titration
Titrant: 0.1 N perchloric acid VS
Nchọpụta njedebe: Potentiometric
Nyocha: Gbarie Sample na 6 ml nke formic acid na 50ml nke glacial acetic acid, na titrate na Titrant.Mee mkpebi nke oghere ahụ.
Gbakọọ pasent tyrosine (C9H11NO3) n'ihe nlele emere:
Nsonaazụ = {[(VS-VB) x N x F]/W} x100
VS = olu Titrant nke Sample (mL) riri.
VB = olu Titrant nke oghere (mL) riri.
N = ezigbo usoro nke Titrant (mEq/ml)
F= ihe nhata, 181.2 mg/mEq
W= Nlereanya arọ (mg)
Usoro nnabata: 98.5% ~ 101.5% na ntọala akpọnwụwo
IHE adịghị ọcha
MKWAGHỊ ihe fọdụrụ <281>: NMT 0.4%
Chloride na sulfate, chloride <221>
Ọkọlọtọ ngwọta: 0.20ml nke 0.020 N hydrochloric acid
Ihe atụ: 0,35 g nke Tyrosine.[IHE-Ọ bụrụ na ọ dị mkpa, gbarie Sample ahụ site na kpo oku na nso esi esi ma tinye 1 mL nke nitric acid.]
Usoro nnabata: NMT 0.04%
chloride na sulfate, sulfate <221>
Ngwọta ọkọlọtọ: 0.50mL nke 0.020 N sulfuric acid
Ihe atụ: 1.2 g nke Tyrosine.[IHE-Ọ bụrụ na ọ dị mkpa, gbarie Sample ahụ site na ịgbakwunye 6 ml nke hydrochloric acid diluted.]
Usoro nnabata: NMT 0.04%
IRON <241>: NMT 30ppm (Ọ bụrụ na ọ dị mkpa, jiri 2 ml nke hydrochloric acid gbarie Sample.)
Ọla dị arọ, Usoro I <231>: NMT 15ppm
Ngwakọta ejikọtara
Ngwọta amonia agbazere: gbanye 16 ml nke amonia etinyere na mmiri na 100 ml.
Ngwọta kwesịrị ekwesị nke sistemu: Gbarie 10 mg nke USP L-Tyrosine RS na 10 mg nke USP L-Phenylalanine RS na 1 mL nke ngwọta amonia diluted, ma gbanye mmiri na 25.0 ml.
Ngwọta ọkọlọtọ: N'ime flask dabara adaba, gbarie ọnụọgụ USP L-Tyrosine RS na 1 mL nke ngwọta amonia Diluted.Jiri mmiri na-atụgharị na olu iji nweta ihe ngwọta nke 0.05mg/mL.[IHE-Ngwọta a nwere ntinye uche ya na 0.5% nke ngwọta Sample.]
Ngwọta nlele: Nyefee 0.1 g nke Tyrosine n'ime flask volumetric 10-mL, gbakwunye ngwọta ammonia zuru ezu ka ọ gbazee, ma gbanye ya na mmiri ka ọ bụrụ olu.
Sistemu chromatographic (Lee Chromatography <621>, Chromatography nke dị obere.)
Ụdị: TLC
Adsorbent: 0.25-mm oyi akwa nke chromatographic silica gel ngwakọta
Olu ngwa: 5μL
Sistemụ ihe mgbaze na-emepe emepe: mmanya isopropyl na ammonium hydroxide (7: 3)
Mgbasa reagent: 2 mg/mL nke ninhydrin na ngwakọta nke mmanya butyl na 2N acetic acid (95:5)
Sistemu kwesịrị ekwesị
Ihe achọrọ dabara adaba: chromatogram nke ngwọta dabara adaba sistemụ na-egosipụta ntụpọ abụọ kewapụrụ nke ọma.
Nyocha
Nlereanya: Ngwọta dabara adaba sistemu, Ngwọta ọkọlọtọ, na ngwọta nlele.
Kpochapụ efere n'etiti 100° na 105° ruo mgbe amonia ga-apụ n'anya kpamkpam.Jiri reagent fesa, wee kpoo ọkụ n'etiti 100 ° na 105 ° maka 15 min.Nyochaa efere n'okpuru ọkụ ọcha.
Usoro nnabata: Ebe ọ bụla nke abụọ nke ngwọta Sample adịghị ibu ma ọ bụ sie ike karịa isi ntụpọ nke Standard Solution.
Ihe adịghị ọcha nke onye ọ bụla: NMT 0.5%
Mkpokọta adịghị ọcha: NMT 2.0%
Nnwale akọwapụtara
Ntụgharị anya, ntụgharị a kapịrị ọnụ <781S>
Ngwọta nlele: 50 mg/mL na 1 N hydrochloric acid
Ụkpụrụ nnabata: -9.8° ruo -11.2°
Ọnwụ na-ehicha <731>: Kpochapụ ihe nlele na 105 ℃ maka awa 3: ọ na-efunahụ NMT 0.3% nke ịdị arọ ya.
NKWUO GBASARA YA
Nkwakọ ngwaahịa na nchekwa: Chekwaa n'ime akpa mechiri emechi nke ọma.
Ụkpụrụ ntụaka USP <11>
USP L-Phenylalanine RS
USP L-Tyrosine RS