Ngwa: Ngwa GC (Shimadzu GC-2010)
Ogidi: DB-17 Agilent 30mX0.53mmX1.0μm
Okpomọkụ mbụ nke oven: 80 ℃
Oge mbu 2.0min
Ọnụ 15 ℃/min
Igwe ọkụ ọkụ ikpeazụ: 250 ℃
Oge ikpeazụ 20min
Nitrogen na-ebu gas
Usoro na-aga mgbe niile
Na-eru 5.0ml/min
Oke nkewa 10:1
Injector okpomọkụ: 250 ℃
Ọnọdụ okpomọkụ: 300 ℃
Olu ogwu ogbugba 1.0μL
Akpachara anya ka a ga-eme tupu nyocha:
1. Ọnọdụ kọlụm na 240 ℃ maka opekempe 30minutes.
2. Sachaa sirinji ma hichaa liner injector nke ọma iji wepụ ihe mmetọ nke nyocha mbụ.
3. Na-asa, kpoo ma jupụta diluent na vial ịsacha sirinji.
Nkwadebe mmiri ara ehi:
Kwadebe 2% w/v sodium hydroxide ngwọta n'ime mmiri.
Nkwadebe ọkọlọtọ:
Tụru ihe dịka 100mg nke (R) -3-hydroxyprolidine hydrochloride ọkọlọtọ n'ime vial, tinye 1mL nke mgbaze wee gbazee.
Nkwadebe nwale:
Were ihe dị ka 100mg nke nlele ule n'ime vial, tinye 1 ml nke ihe na-esi ísì ụtọ ma gbazee.Kwadebe na oyiri.
Usoro:
Tinye oghere (diluent), nkwadebe ọkọlọtọ na nkwadebe ule site na iji ọnọdụ GC dị elu.Eleghara ọnụ ọgụgụ ndị ahụ anya n'ihi oghere.Oge njide kacha elu n'ihi (R) -3-hydroxyprolidine bụ ihe dịka 5.0min.
Mara:
Kwuo nsonaazụ dịka nkezi
Oge nzipu: Nov-13-2021