Nnu Phthalimide Potassium CAS 1074-82-4 Dị Ọcha>99.0% (HPLC)

3. Usoro ule
3.1 Mfu na ihicha (W/W)
3.1.1 Akụrụngwa na ngwa
Otu ihe karịrị puku iri nke itule eletrọnịkị, ọkụ ọkụ ọkụ ọkụ ọkụ, karama na-atụ 40 * 25, igwe nkụ.
Reagent: silica gel
3.1.2 Ụzọ
1. Kpochapụ karama na-atụ 3 40 * 25.
2, tinye na oven na 105 ℃ (± 2 ℃) nkụ maka 1 hour.
3. Wepụ karama na-atụ 40 * 25 ma tinye ya n'ime igwe nkụ ka ọ dị jụụ maka 30min.
4, Ire nha 40*25 karama na-atụ ihe efu W0.
5. Jiri karama na-atụ 40 * 25 tụọ nha nha nha nha nke 3.0000g (W1), ngụkọta nke 3.
6, Tinye na oven, wepụ karama okpu, tinye ya n'akụkụ karama na-atụ, ma ọ bụ ọkara na-emeghe karama okpu maka ihicha, wepụ, ga-adị mma na-atụ karama okpu, akọrọ na 105 ℃ (± 2℃) maka. awa 3.
7. Wepụ karama ahụ 40 * 25 ma tinye ya n'ime ngwaọrụ ihicha ka ọ dị jụụ maka 30min.
8, Nri ziri ezi mgbe ihicha 40 * 25 arọ karama W2.
Usoro ngụkọ: Mfu na ihicha (%) =(W1-W2)/(W1-W0)×100%
3. 4 Mkpebi ọdịnaya
3.4.1 Ngwa na akụrụngwa
LC-UV100 Liquid Chromatograph (Shanghai Wufeng Scientific Instrument Co., LTD.)
10ml volumetric kalama, igwe anaghị agba nchara ngaji na ihe ndị ọzọ metụtara ya
3.3.2 Ụzọ
Usoro nyocha nke Phthalimide Potassium nnu
3.3.3 chromatography mmiri
Ọnọdụ chromatographic
Kọlụm Chromatographic: HypersilC6H5 (C8 dịkwa) 4.6*250mm 5um
Ogologo oge nchọpụta: 210nm
Okpomọkụ kọlụm: 30 ℃
Ọsọ ọsọ: 1.0ml/min
Nleta nlele: 0.05mg/ml (diluent bụ usoro mkpanaka)
Reagents
Potassium Phosphate Dibasic (nyocha dị ọcha)
Nnu Phthalimide Potassium (ihe atụ ọkọlọtọ)
Acetonitrile (nyocha dị ọcha)
Mmiri dị ọcha
Usoro mkpanaaka: acetonitrile: ihe nchekwa phosphate (PH:3.5) =40:60
Na-atụ 1.63g Potassium Phosphate Dibasic na igbari na 600ml mmiri dị ọcha, mezie PH uru na 3.5 na phosphoric acid, wụsa n'ime 1000ml beaker nwere 400ml acetonitrile, mix ọma, nyo na 0.45um mix fiber filter membrane, igbari na njikere na ultrasonic vibration. ngwa.
3.3.4 Usoro
1) Ngwọta nyocha nhazi (0.05mg / ml)
A tụrụ ihe nlele 2.5mg nke ọma ma tinye ya na karama volumetric 50ml.A na-agbakwunye usoro mkpanaka ma gbanye ya na ọnụ ọgụgụ site na mgbasa ultrasonic.
2) Ngwọta ọkọlọtọ nhazi (1.0mg / ml)
A tụrụ atụ ọkọlọtọ 2.5mg nke ọma ma tinye ya na karama volumetric 50ml.A na-agbakwunye usoro mkpanaka ma gbanye ya na ọnụ ọgụgụ site na mgbasa ultrasonic.
3) Iji sistemu arụ ọrụ:
(1) Tinye isi nzacha n'ime 80% methanol na mmalite, mepee valve iyuzucha na igbapu maka 3-5min, ma mechie valvụ iyuzucha ma kpochaa maka 10-15min.Mgbe ahụ, tinye isi nzacha n'ime mmiri dị ọcha 90%, mepee valvụ drain na igbapu maka 3-5min, mechie valvụ drain ahụ ma kpochaa maka 10min.
(2) Mgbe ahụ, gbanwee usoro mkpanaka a kwadebere, ọnụ ọgụgụ na-agba ọsọ bụ 1.0ml / min, nrụgide n'ihu kọlụm bụ ihe na-erughị 1000Psi, mepee nchọpụta ahụ, chere ruo mgbe nrụgide n'ihu kọlụm kwụsiri ike, mepee ọrụ kọmputa, wee banye na ntanetị.
(3) Mgbe nrụgide n'ihu kọlụm kwụsiri ike, mepee ihe nchọpụta.Buru ụzọ gbanye agịga 1 nke usoro mkpanaaka, wee gbanye agịga 1 nke ọkọlọtọ ọkọlọtọ, wee gbanye sample.
(4) Nsonaazụ mgbako: usoro nhazi mpaghara
(5) Mgbe ịmechara ọrụ nyocha, mechie onye nchọpụta mbụ, wee kpochaa isi nzacha na mmiri dị ọcha maka 10-15min;N'otu oge ahụ, a na-asacha valvụ injection na mmiri na methanol.
(6) N'ikpeazụ, tinye methanol 80%, sachaa maka 30min, wee mechie.
4. Mgbe emechara nyocha, dejupụta akwụkwọ nyocha na ụlọ nyocha.