N-Acetyl-L-Tyrosine CAS 537-55-3 (Ac-Tyr-OH; NALT)

N-Acetyl-L-Tyrosine (Ac-Tyr-OH; NALT) (CAS: 537-55-3) AJI97 Methodus Test.
N-Acetyl-L-Tyrosina siccata, non minus quam 99.0 centesimas continet et non plus quam 101.0 centesimas N-Acetyl-L-Tyrosinae (C11H13NO4).
Description: Crystalla alba vel pulveris crystallini, gustus acidus valde
Solutum in aqua.
Solubilitas (H2O, g/100g): 17.5 (20℃), 72.4 (40℃), 158 (60℃).
Lepidium sativum: Confer spectrum ultrarubrum effusio exempli cum illa regulae per modum discus bromide potassium.
Imprimis rotationis [α]20/D: specimen siccatum, C=4, H2O
Status Solutionis (Transmittance): 0.5g in 10ml H2O, spectrophotometri, 430nm, 10mm cellulae crassitudinis.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml of 0.01mol/L HCl
Aconitum (NH4): B-1
Sulfate (SO4): 1.2g, (1), ref: 0.50ml ex 0.005mol/L H2SO4
Ferrum (Fe): 0.5g, ref: 1.5ml de Iron Std.(0.01mg/ml)
Metalla gravia (Pb): 2.0g, (3), ref: 2.0ml of Pb Std.(0.01mg/ml)
Arsenicum (As2O3): 1.0g, (1), ref: 2.0ml de As2O3 Std.
Damnum in remittit: ad CV "pro III horas"
Residere in Ignitione (Sulfated): AJI Test 13
Intenta: Arida specimen, 300mg, (1), 40ml H2O, (2), 0.1mol/L NaOH, 1ml=22.323mg C11H13NO4
Tyrosine: Liquida fina.Conditio operans: Detector: UV effusio photometer (214nm);Columna: ODS, 4.6mm x 150mm;Columna tortor: volutpat tortor.Fluunt rate: 1ml/min;Mobilis periodus: 0.04mol/L solutio monopotassii phosphatis (pH 4.35): Methanolum (193: 3).
pH: 1.25g in 50ml H2O
Repono limitem et conditionem commendatur: Praeservata continentia stricta ad locus temperatus sobrie (2 annis).

N-Acetyl-L Tyrosine (Ac-Tyr-OH; NALT) (CAS: 537-55-3) USP35 Methodus Test.
DEFINITIO
N-Acetyltyrosinum continet NLT 98,5% et NMT 101.0% C11H13NO4, ut N-Acetyl-L-Tyrosinum, in fundamento siccato computatum.
IDENTIFICATIO
A. INFRARED HAUSTUS <197K>
B. ROTATIO OPTICALIS, Rotationis Imprimis <781S>
Sample solutio: 10 mg/mL
Re- gulae acceptationis: NLT +46.0° et NMT +49.0°, determinatae ad 20°
C. RF valor principalis maculae solutionis Sample in tium immunditiarum organicarum respondet solutioni Latin 1 .
TEMPTATIO
MODO PROCEDENDI
Sample solutio: Dissolve circa 180 mg de N-Acetyltyrosine, appensum in 50 mL aquae dioxidis liberae.
Systema titrimetricum
(Vide Titrimetrium<541>)
Modus: Direct titration
Titrant: 0.1 N sodium hydroxidum VS
Endpoint deprehensio: Potentiometric
Equivalentia: Utraque mL of 0.1 N sodium hydroxidi VS aequivalet 22.32 mg C11H13NO4.
IMPURITIES
Inorganic immunditias
RESIDUEON IGNIS <281>: NMT 0.1%
CHLORIDE ET SULFATE, CHLORIDIS <221> NMT 200 ppm.Pars 0.7-g non plus chloridi ostendit quam 0,40 mL 0.01 Acidi hydrochlorici N.
CHLORIDE ET SULFATE, Sulfate <221> NMT 200 ppm.Pars 1.2-g A 1.2-g non plus ostendit sulphate quam 0,25 mL 0,020 Acidi sulphurici N correspondens.
FERRUM <241>: NMT 20ppm
GRAVALIS METALS, Ratio I <231>: NMT 10ppm
Impurities organica
MODO PROCEDENDI
Adsorbent: 0.25-mm iacuit chromatographico silicae gel mixtionis
Solutio generum stirpis 1: 8 mg/mL of USP N-Acetyl-L-tyrosinus RS in mixtione aquae, acidum aceticum glaciale, et alcohol (3:3:94)
Solutio Standard: Dilutire solutionem Latin stock 1 cum alcohol ad solutionem obtinendam habens notam intentionem circiter 0.4 mg/mL
Solutio Standard: 0.8 mg/mL USP L-tyrosinae RS in mixtione acidi glacialis acetici et aquae (1:1), et alcohol diluti.
Sample solutio: Transfer 0.8 g of N-Acetyltyrosine ad 10-mL lagena voluminis, dissolve in 6 mL mixtionis acidi glacialis et aquae (1:1), et alcohole ad volumen dilutum.
Volumen: 5 µL
Solvendo ratio explicandi: Mixtura ammoniaci et 2-propanoli (3:7).
Imbre reagens: Dissolve 0,2 g ninhydrin in 100 mL mixtionis butanol et 2 N acetici (95:5).
Ratio convenientia
Opportunitas requisita: solutio chromatogramma Systematis convenientia ostendit duas maculas distincte separatas.
Analysis: Perge uti ad Chromatographiam <621> Thin-Laer Chromatographiam.Post laminam aer exsiccans, processus evolutionis iterare.Post aerem secundo tempore siccatio, imbre cum Spray gerente, et calor inter 100° et 105° circiter 15 min.
De acceptatione criteria: Quaelibet macula secundaria Sample solutionis non maior vel intensior est quam principali macula solution1 in Latin, excepto loco tyrosine correspondens, qui non est maior vel vehementior quam principal macula from Latin solution II.
Singulae immunditiae: NMT 0.5%
Terminus tyrosine: NMT 1.0%
IN SPECIE TESTS
LOSSON EXsiccatio <731>: sicca specimen in 105℃ pro 3 h: amittit NMT 0,1% sui ponderis.
ADDITICIUS REQUISITIS
PACKAGING AND STORAGE: Conserva in vasis bene clausis et repone ad temperatura cella moderata.
USP Reference STANDARDS <XI>
USP N-Acetyl-L tyrosine RS
USP L-Tyrosine RS