Adenosine CAS 58-61-7 Assay 99.0% -101.0% USP Paerewa Factory High Pure

WHAKAMAHI
Kei roto i te Adenosine te NLT 99.0% me te NMT 101.0% o C10H13N5O4, kua tatauhia i runga i te whakamaroke.
WHAKATAUTANGA
• A. KAUPAPA INFRARED <197K>: NMT 0.1%
WHAKAMAHI
• ADENOSINE
Tauira: 200 mg o Adenosine i maroke i mua i te 105° mo te 2h
Pūnaha Titrimetric
(Tirohia Titrimetry <541>)
Aratau: Te titration tika
Titrant: 0.1 N ia waikawa maota VS
Te kitenga ira mutunga: Potentiometric
Tātaritanga: Whakarewa i roto i te 50 mL o te waikawa ahetiki glacial ka titrate ki te 0.1 N mo ia waikawa maota VS.Tātaihia te ōrau o te Adenosine (C10H13N5O4) i te wahanga i tangohia:
Hua = [(V − B) × N × F × 100]/W
V = Tauira titrant rōrahi (mL)
B = rōrahi titrant pātea (mL)
N= te tikanga titorehanga (mEq/mL)
F= tauwehe taurite: 267.25 mg/mEq
W= te taumaha o te Tauira (mg)
Paearu whakaae: 99.0% -101.0% i runga i te whakamaroke
• NGA POKE
• MAHI RANGI <281>: NMT 0.1%
• NGA METAL TAHA, Tikanga II <231>: NMT 10 ppm
• TE TEITI O AMMONIA
Tauira otinga: Whakatarewa 0.5 g ki te 10 mL wai.Oho mo te 30 s, ka haere i roto i te tātari taratara.Ringihia te filtrate ki te wai ki te 15 mL, ka whakamahi i te filtrate.
Wairewa paerewa: 0.4 µg/mL o te haukinia pūhaumāota i roto i te wai
Tātaritanga: Ki te Tauira otinga me te otinga Paerewa tāpirihia te 0.3 mL o te kawakore mer curic–te konukawata iodide TS, ka kapi i nga ipuipu, ka waiho kia tu mo te 5 meneti.
Paearu whakaae: Ko te otinga Tauira karekau e whakaatu he tae kowhai kaha ake i tera o te otinga Paerewa (NMT 4 ppm o te haukini).
• TE TEITI O CHLORIDE
Tauira otinga: Whakatarewa 0.2 g ki te 10 mL wai.Whakakotahitia mo te 30 hēkona, haere i roto i te tātari taratara, ka whakamahi i te tātari.
Wairewa paerewa: 2.3 µg/mL konutai pūhaumāota i roto i te wai
Tātaritanga: Ki te Tauira wairewa me te 10 mL o te wairewa Paerewa tāpirihia te 1 mL o te waikawa nitric me te 1 mL o te hiriwa pākawa TS, ka waimeha ia wairewa ki te 40 mL.Whakaaetia nga otinga kia tu mo te 5 meneti, ka tiakina mai i te marama.
Paearu whakaae: Ina tirohia ki te papamuri pouri, ko te otinga Tauira karekau e nui ake i te wairewa Paerewa (NMT 0.007% chloride).
• TE TEITI O TE PUKA
Tauira otinga: Whakatarewa 0.75 g ki te 15 mL wai.Whakakotahitia mo te 30 hēkona, haere i roto i te tātari taratara, ka whakamahi i te tātari.
Te otinga paerewa: Tāpirihia te 0.15 mL o te 0.020 N te waikawa pungatara ki te 15 mL wai.
Tātaritanga: Ki te Tauira otinga me te otinga Paerewa tāpirihia te 2 mL o te barium chloride TS me te 1 mL o te 3 N waikawa pūhaumāota, waimeha ia wairewa ki te 30 mL, ka uru.Tukua nga otinga kia tu mo te 5 min.
Paearu whakaae: Ko te otinga Tauira e kore e nui ake i te wairewa Paerewa (NMT 0.02% sulfate).
• NGĀ PORO ORGANIC
Rongoā A: 6.8 g/L o te pāhare pāporo hauwai pūkawa ngāwha me te 3.4g/L o te tetrabutylammonium hauwai pūkawa ngāwha i roto i te wai.Whakaritea ki te 2 N pāhare pāporo waihā ki te pH o 6.5.
Rongoā B: 0.1 g/L o te wairewa konutai azide
Wāhanga pūkoro: Rongoā A me Rongoā B (60:40)
Te otinga pai o te punaha: 0.2 mg/mL ia Adenosine me te inosine i te wahanga Waea
Tauira otinga: 1.0 mg / mL o Adenosine i roto i te waahanga Waea
Pūnaha Chromatographic
(Tirohia te Chromatography <621>, Whakaritenga Pūnaha.)
Aratau: LC
Kaitirotiro: UV 254 nm
Tīwae: 4.6-mm × 25-cm;5-µm tākai L1
Rere rere: 1.5 mL/min
Rahi werohanga: 20 µL
Te pai o te punaha
Tauira: Te otinga pai o te punaha
Nga whakaritenga e tika ana
Whakatau: NLT 9.0 i waenga i te adenosine me te inosine
Te take hiku: NMT 2.5
Inenga Paerewa Paanga: NMT 2.0%
[FAKATOKANGA-Chromatograph te Tauira otinga, ka whakatika i te wa oma kia rua neke atu i te wa pupuri o te tihi nui.]
Tātaritanga
Tauira: Tauira otinga
Tātaihia te ōrau o ia poke i te wahanga o te Adenosine i tangohia:
Hua = (rU/rT) × 100
rU = te whakautu teitei o ia poke mai i te otinga Tauira
rT = te tapeke o nga whakautu katoa mo nga tihi katoa mai i te Tauira otinga
Paearu whakaae
Nga poke takitahi: NMT 0.1% o ia guanosine, inosine, me te uridine, me te NMT 0.2% o te adenine
Tapeke poke: NMT 0.5%
NGA WHAKAMATAUTANGA
• AWE REREWA RĀNEI TE MAMAHA <741>: 233°-238°
• TE WHAKATAUTANGA OPTICAL, Hurihanga Tauwhāiti <781S>: -68° ki te -72°
Te otinga whakamatautau: 20 mg/mL i roto i te wairewa konutai waikawa (1 i roto i te 20), ka whakatauhia ki runga i tetahi tauira i whakamarokehia i mua i te 105° mo te 2h
KAAWAKA, KAUPAPA KAUPAPA ranei: Whakatarewa 1 karaehe ki roto i te 20 mL o te wai waro-kore-kore.Oho mo te 30 s, ka haere i roto i te tātari taratara.Ki ia o nga wahanga 10-mL e rua o te filtrate me tapiri 0.1mL o te bromocresol papura TS.
Paearu whakaae: NMT 0.3 mL o te 0.01 N konutai waikawa e hiahiatia ana kia puta he tae puru-puru i roto i te waahanga kotahi.NMT 0.1 mL o te 0.01 N waikawa pūhaumāota e hiahiatia ana kia puta he tae kowhai ki tera atu wahanga.
•MOROKE MORE <731>: Me whakamaroke he tauira ki te 105 ℃ mo te 2 haora: ka ngaro te NMT 0.5% o tona taumaha.
HE WHAKAMAHI ATU
•PAKITANGA ME TE WHAKAPUTANGA: Whakaorangia ki roto i nga ipu kikii, kia kore e marama, ka penapena ki te pāmahana rūma whakahaere.
• USP REFERENCE STANDARD <11>
USP Adenosine RS