L-Glutamic Acid α-Benzyl Ester CAS 13030-09-6 (H-Glu-OBzl) Assay >98.5% (HPLC) Kounga Teitei

1. Pure (HPLC)
1.1 Taputapu
Ko te chromatography wai mahi teitei, te kaitao UV3000.
Taurite tātari hiko
1.2 Reagents
Acetonitrile (koeke chromatographic), TFA (koeke chromatographic)
1.3 Nga tikanga Chromatographic
1.3.1 Tīwae: tīwae C18, 5 μl * 250 * 4.6 mm
1.3.2 Te roangaru rapu: 220nm
Rere rere: 1.0 mL/min
Rōrahi werohanga:10 μL(mo te tohutoro)
Whakarewa: acetonitrile
Wā oma: 20 meneti
1.4 Whakaritenga wahanga pūkoro
Wāhanga pūkoro A (0.1% Trifluoroacetic acid Water): kapo tika i te 1.0 ml TFA kua diluted ki te 1000 ml ki te wai, te ranunga me te degas.
Wāhanga pūkoro B (0.1% Trifluoroacetic acid Acetonitrile): kapo tika i te 1.0 ml TFA, ka diluted ki te 1000 ml me te acetonitrile, te ranunga me te degas.
1.5 Hōtaka Rōnaki
Wā (min) A% B%
0:00 90 10
10.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
1.6 Te whakarite tauira otinga
Paunatia te 0.1 g tauira ka rewa ki te acetonitrile ka waimeha ki te 100ml.Ruperupea pai hei whakamahi a muri ake nei, kia rite ranei te kukū.Whakaritea kia rua nga tauira hei whakarara.
1.7 Tauira Whakatau
Tātarihia te tauira kia rite ki te tikanga tauira e whai ake nei:
1) Neke atu i te 1 werohanga o te otinga patea
2) 1 werohanga o te otinga tauira 1#
3) 1 werohanga tauira otinga 2#
1.8.0 Tatautanga
1.8.1 I whakamahia te tikanga whakatikatika rohe tihi ki te tatau i te parakore o te HPLC.
1.8.2 Kaua e nui ake i te 1%.
1.8.3 Mena ka tutuki nga hua o nga werohanga e rua ki nga paearu whakaae, tangohia te maatanga toharite hei hua whakamutunga.