Waikawa Chenodeoxycholic (CDCA) CAS 474-25-9 Assay ≥98% (Tuku maroke)

Waikawa Chenodeoxycholic
C24H40O4: 392.57
3a,7a-Dihydroxy-5b-cholan-24-oic acid [474-25-9]
Ko te Chenodeoxycholic Acid, ina whakamarokehia, kaua e iti iho i te 98.0z me te kore e neke ake i te 101.0% o C24H40O4.
Whakaahuatanga Ko te waikawa Chenodeoxycholic ka puta he ma, he tioata, he paura tioata, he paura ranei.
He waatea noa ki te mewaro me te waiwaro (99.5), ka whakarewahia ki te acetone, me te kore e rewa ki te wai.
Te Tautuhinga Whakatauhia te tuāwhiorangi whakauru infrared o Chenodeoxycholic Acid, i whakamarokehia i mua, pera i te whakahau i roto i te tikanga kōpae pāhare pāporo bromide i raro i te Spectrophotometry Infrared <2.25>, ka whakataurite i te tūāwhiorangi ki te Tohu Tohutoro: e whakaatu ana nga tūāwhiorangi e rua i te kaha o te horonga i te tau ngaru kotahi.
Te hurihanga whatu <2.49> [a]20D : +11.0 °~ +13.0° (i muri i te whakamaroketanga, 0.4 g, ewaro (99.5), 20 mL, 100 mm).
Ira whakarewa <2.60> 164~169 ℃
Purity (1) Chloride <1.03>-Warekahia te 0.36 g o te Chenodeoxycholic Acid ki te 30 mL o te methanol, tāpirihia te 10 mL o te waikawa nitric dilute me te wai kia 50 mL, ka mahia te whakamatautau me tenei otinga.Whakapaia te otinga whakahaere penei: Ki te 1.0 mL o te 0.01 mol/L waikawa pūhaumāota VS tāpirihia te 30 mL o te mewaro, 10 mL o te waikawa nitric dilute me te wai kia 50 mL (kaua e neke ake i te 0.1%).
(2) Nga konganuku taumaha <1.07>-Haere ki te 1.0 g o te Chenodeoxycholic Acid kia rite ki te Tikanga 4, ka mahi i te whakamatautau.Whakapaia te otinga mana me te 2.0 mL o te Wairewa Aratohu Paerewa (kaua e neke ake i te 20 ppm).
(3) Barium-Ki te 2.0 g o te Chenodeoxycholic Acid ka tapirihia te 100 mL wai, ka kohuatia mo te 2 meneti.Ki tenei wairewa tāpirihia te 2 mL waikawa pūhaumāota, kohuatia mō te 2 meneti, tātari i muri i te mātao, ka horoi i te tātari ki te wai tae noa ki te 100 mL o te filtrate.Ki te 10 mL o te filtrate tāpirihia te 1 mL o te waikawa sulfuric waimeha: karekau he kopouri.
(4) Nga matū e pa ana-Whakarewahia te 0.20 g o te Chenodeoxycholic Acid ki te ranunga o te acetone me te wai (9:1) kia rite ki te 10 mL, ka whakamahi i tenei otinga hei tauira otinga.Whakarewa motuhake, 10 mg o te waikawa lithocholic mo te chromatography paparanga angiangi ki te ranunga o te acetone me te wai (9:1) kia rite ki te 10 mL.Pipeto 2 mL o tenei wairewa, tapirihia te ranunga o te acetone me te wai (9:1) kia rite te 100 mL, ka whakamahi i tenei otinga hei otinga paerewa (1).Ka wehea te 10 mg o te waikawa ursodeoxycholic ki te ranunga o te acetone me te wai (9:1) kia rite ki te 100 mL, ka whakamahi i tenei otinga hei otinga paerewa (2).Whakarewa motuhake te 10 mg o te waikawa cholic mo te chromatography paparanga angiangi ki te ranunga o te acetone me te wai (9:1) kia rite ki te 100 mL, ka whakamahi i tenei otinga hei otinga paerewa (3).Pipeto 1 mL o te tauira otinga, ka tapirihia te ranunga o te acetone me te wai (9:1) kia rite te 20 mL.Pipeto 0.5 mL, 1 mL, 2 mL, 3 mL me te 5 mL o tenei wairewa, tapirihia te ranunga o te acetone me te wai (1) ki ia o ratou kia rite ki te 50 mL, ka tohua enei rongoa hei otinga A, otinga paerewa. B, otinga paerewa C, otinga paerewa D me te otinga paerewa E, ia.Mahia te whakamatautau me enei otinga e kiia ana i raro i te Thin-layer Chromatography<2.03>.Tuhia te 5 mL o ia tauira otinga, wairewa paerewa (1), (2), (3) me nga otinga paerewa A, B, C, D me E ki runga i te pereti kirikiri mo te chromatography paparanga angiangi.Whakawhanakehia te pereti ki te ranunga 4-methyl-2-pentanone, toluene me te waikawa puka (16:6:1) ki te tawhiti 15 cm, whakamaroke te pereti, ka whakamaroke ano i te 1209C mo te 30 meneti.I te wa tonu, rehuhia he otinga o te waikawa phosphomolybdic n-hydrate i roto i te waihano (95) (1 i roto i te 5) ki runga i te pereti, ka whakamahana i te 120 ℃ mo te 2 ki te 3 meneti: te waahi e rite ana ki te waahi me te otinga paerewa (1) kaore i te kaha ake i te waahi me te otinga paerewa (1), te waahi e rite ana ki te waahi me te otinga paerewa (2) kaore i te kaha ake i te waahi me te otinga paerewa (2), me te waahi e rite ana ki te waahi. me te otinga paerewa (3) kaore i te kaha ake i te waahi me te otinga paerewa
(3).Ina whakaritea ki nga waahi me nga otinga paerewa A, B, C, D me E, ko nga waahi i tua atu i te waahi matua me era atu i nga waahi kua whakahuahia i runga ake nei kaore i te kaha ake i te waahi me te otinga paerewa E, me te tapeke moni. o ratou kaua e neke ake i te 1.5%.
Ngaronga i te whakamaroke <2.41> Kaua e neke ake i te 1.5%(1 g, 105℃,3 haora).
Te toenga i runga i te mura <2.44> Kaua e neke ake i te 0.1% (1 g).
Whakamaatau Kia tika te pauna mo te 0.5 g o te Chenodeoxycholic Acid, kua whakamarokehia i mua, memeha ki te 40 mL o te waihano (95) me te 20 mL wai, me te titrate <2.50> ki te 0.1 mol/L konutai hydroxide VS (potentiometric titration).Kia rite tonu te mahi i tetahi whakatau paatea, me te whakatika i nga mea e tika ana.
Ia mL o te 0.1 mol/L konutai waihā VS ＝ 39.26 mg o C24H40O4
Ipu me te rokiroki Ipu-Tight ipu.