Glycine CAS 56-40-6 (H-Gly-OH) Assay 98.5~101.5% Factory High Quality

USP35-NF30
WHAKAMAHI
Kei roto i te Glycine te NLT 98.5% me te NMT 101.5% o te glycine (C2H5NO2), ka tatauhia i runga i te whakamaroke.
WHAKATAUTANGA
A. KAUPAPA INFRARED <197M>
WHAKAMAHI
• TE WHAKAMAHI
Tauira: 150 mg o Glycine
Putea: 100 mL waikawa ahetiki hukapapa
Pūnaha Titrimetric
(Tirohia Titrimetry <541>.)
Aratau: Te titration tika
Titrant: 0.1 N ia waikawa maota VS
Te kitenga ira mutunga: Ataata
Tātaritanga: Whakarewahia te Tauira ki te 100 mL o te waikawa ahetiki glacial, ka tāpirihia te 1 topata o te karaihe puru TS.Whakawetohia me te Titrant ki te pito kaakaariki.Mahia te whakatau Patea.
Tātaihia te ōrau o te glycine (C2H5NO2) i te Tauira i tangohia:
Hua = {[(VS − VB) × N × F]/W} × 100
VS = rōrahi titrant i pau i te Tauira (mL)
VB = rōrahi titrant i pau i te Patea (mL)
N = te noho noa o te Titrant (mEq/mL)
F = tauwehe taurite, 75.07 mg/mEq
W = Tauira taumaha (mg)
Paearu whakaae: 98.5% -101.5% i runga i te whakamaroke
NGA POKE
• TOHUTOHU I RUNGA I TE MAHI <281>: NMT 0.1%
• KORERO ME TE PUKA, KORERO <221>
Wairewa Paerewa: 0.10 mL o te 0.020 N waikawa pūmāota
Tauira: 1 g Glycine
Paearu whakaae: NMT 0.007%
• CHLORIDE AND SULFATE, Sulfate <221>
Wairewa paerewa: 0.20 mL o te 0.020 N te waikawa pungatara
Tauira: 3 g o Glycine
Paearu whakaae: NMT 0.0065%
• NGA METAL TAHA, Tikanga I <231>: NMT 20 ppm
• NGA METAL TAHA, Tikanga I <231>: NMT 20 ppm
Tauira otinga: 100 mg/mL o Glycine
Tātaritanga: Kohuatia te 10 mL o te otinga Tauira mo te 1 meneti, ka waiho mo te 2 haora.
Paearu whakaae: Ko te otinga he maamaa me te waea pūkoro rite ki te 10 mL o taua otinga kaore ano kia kohuatia.
NGA WHAKAMATAUTANGA
• NGARO I TE WHAKAMAROKE <731>: Me whakamaroke he tauira ki te 105° mo te 2 haora: ka ngaro te NMT 0.2% o tona taumaha.
HE WHAKAMAHI ATU
• TE PAKITANGA ME TE WHAKAPUTANGA: Whakaorangia ki roto i nga ipu kua kati pai.
• USP REFERENCE STANDARD <11>
USP Glycine RS

Hapanihi Pharmacopoeia JP17
Ko te Glycine, ina whakamaroke, kaua e iti iho i te 98.5% o Glycine(C2H5NO2).
Whakaahuatanga Ka puta te Glycine he ma, he tioata orcrystalline paura.He reka te reka.He memeha noa ki te wai me te waikawa formic, me te kore e rewa ki te waiwaro (95).E whakaatu ana i te polymorphism tioata.
Te Tautuhinga Whakatauhia te tuāwhiorangi whakauru infrared o Glycine, i whakamarokehia i mua, pera i te tohutohu i roto i te tikanga kōpae pāhareporoporoporo i raro i te Spectrophotometry Infrared <2.25>, ka whakatairitehia te tuāwhiorangi ki te Tohu Tohutoro: e whakaatu ana nga hihinga e rua i te kaha o te horonga i te tau ngaru kotahi.Mena ka puta he rerekee i waenga i te hianga, whakamotitia te Glycine ki te wai, whakaeto te wai kia maroke, ka tuaruatia te whakamatautau me te toenga.
pH <2.54> Whakarewahia te 1.0 g Glycine ki te 20 mL wai: ko te pH o te otinga kei waenganui i te 5.6 me te 6.6.
Maamaa
(1) Te marama me te tae o te otinga-Tarekahia te 1.0 gof Glycine ki te 10 mL wai: he maamaa te otinga, he iti te tae.
(2) Chloride <1.03>-Mahia te whakamatautau me te 0.5 g o Glycine.Whakaritehia te otinga whakahaere me te 0.30 mL o te 0.01mol/L waikawa pūhaumāota VS (kaua e neke ake i te 0.021%)
(3) Sulfate <1.14>-Mahia te whakamatautau me te 0.6 g o Glycine.Whakaritehia te otinga whakahaere me te 0.35 mL o te 0.005mol/L waikawa pungatara VS (kaua e neke ake i te 0.028z).
(4) Ammonium <1.02>-Mahia te whakamatautau ma te whakamahi i te 0.25 g o Glycine.Whakapaia te otinga mana me te 5.0 mL o te Waihanga Haukini Paerewa (kaua e neke ake i te 0.02%).
(5) Heavy Metals <1.07>-Haere ki te 1.0 g o Glycine kia rite ki te Tikanga 1, ka mahia te whakamatautau.Whakapaia te otinga mana me te 2.0 mL o te Wairewa Aratohu Paerewa (kaua e neke ake i te 20 ppm).
(6) Arsenic <1.11>-Whakaritea te otinga whakamatautau me te 1.0 gof Glycine kia rite ki te Tikanga 1, ka mahia te whakamatautau (kaua e neke ake i te 2 ppm).
(7) Nga Waahanga Paa-Whakarewa 0.10 g Glycine ki te 25mL wai ka whakamahi i tenei otinga hei tauira otinga.Pipeto 1 mL o te tauira otinga, tāpirihia he wai kia 50 mL.Pipeto 5 mL o tenei wairewa, tapirihia he wai kia rite ki te 20 mL, ka whakamahi i tenei otinga hei otinga paerewa.Mahia te whakamatautau me enei rongoatanga kia rite ki te tohutohu i raro i te Thin-layer Chromatography<2.03>.Tuhia te 5mL o ia tauira otinga me te otinga paerewa ki runga i te pereti kirikiri kirikiri chromatography.Whakawhanakehia te pereti me te ranunga o te 1-butanol, te wai me te waikawa acetic (100) (3:1:1) ki te tawhiti o te 10 cm, ka whakamaroke i te pereti i te 80 ℃ mo te 30 meneti.Maurihia he wairewa ninhydrin i roto i te acetone (1 i roto i te 50), ka whakamahana i te 80 ℃ mo te 5 meneti: ko nga wahi i tua atu i te waahi matua mai i te tauira otinga kaore i te kaha ake i te waahi mai i te otinga paerewa.
Ngaronga i te whakamaroke <2.41> Kaua e neke ake i te 0.30% (1 g, 105℃, 3 haora).
Te toenga i runga i te mura <2.44> Kaua e neke ake i te 0.10% (1g).
Whakamaatau Kia tika te pauna mo te 80 mg o Glycine, kua maroke i mua, memeha ki te 3 mL o te waikawa formic, tāpirihia te 50 mL o te waikawa acetic (100), ka titrate <2.50> ki te 0.1 mol/L perchloric acid VS (potentiometric titration).Whakaritehia he iwi kore, ka whakatikahia.
Ia mL o te 0.1 mol/L waikawa perchloric VS＝7.507 mg o C2H5NO2
Ipu me te rokiroki Ipu-Kati pai nga ipu.