Histamine Dihydrochloride CAS 56-92-8 Assay 98.5~101.0% (Titration) Factory

Histamine Dihydrochloride (CAS: 56-92-8) EP9.0 VolumeⅡ Tikanga Whakamatau
WHAKAMAHI
Ko te histamine dihydrochloride he iti iho i te 98.5 paiheneti, kaua hoki e neke ake i te rite o te 101.0 ōrau o te 2-(1H-imidazol-4-yl)ethan-1-amine dihydrochloride, he mea tatau ki te matū maroke.
KAUPAPA
He ma, he tata ma ranei, he paura tioata, he kiriata kore tae ranei, he hygroscopic, he tino memeha ki te wai, he memeha ki te waipiro.
WHAKATAUTANGA
Te tohu tuatahi: A, D.
Tautuhi tuarua: B, C, D
A. Te tirotiro ma te infrared absorption spectrophotometry (2.2.24), te whakatairite ki te awhi i whiwhi me te histamine dihydrochloride CRS.Tirotirohia he kopae kua whakaritea ma te whakamahi i te 1 mg o te matū.
B. Tirohia nga chromatograms i whiwhi i te whakamatautau mo te histidine.Ko te waahi matua o te chromatogram i whiwhi ki te otinga whakamatautau (b) he rite te tuunga, te tae me te rahi ki te waahi matua o te chromatogram i whiwhi ki te otinga tohutoro (a).
C. Whakarewahia te 0.1 g ki te 7 mL wai R me te taapiri i te 3 mL o te 200 g/L wairewa konutai waikawa R. Whakarewahia te 50mg o te waikawa sulfanilic R ki te ranunga o te 0.1 mL o te waikawa maaota R me te 10 mL o te wai R me tāpirihia te 0.1 mL o te wairewa konutai nitrite R. Tāpirihia te otinga tuarua ki te otinga tuatahi ka uru.Ka puta he tae whero.
D. Ka hoatu te tauhohenga (a) o nga pūhaumāota (2.3.1).
WHAKAMAHI
Otinga S. Whakarewahia te 0.5g ki te wai waro haukore-kore R i mahia mai i te wai maturu R ka waimeha ki te 10 mL me te whakarewa ano.
Te ahua o te otinga.He maamaa te Otinga S (2.2.1) me te kore e kaha ake te tae i te otinga tohutoro Y7 (2.2.2, Tikanga II).
pH (2.2.3).Ko te pH o te otinga S he 2.85 ki te 3.60.
Histidine.Tirohia ma te chromatography paparanga angiangi (2.2.27), ma te whakamahi i te TLC silica gel G plate R.
Te otinga whakamatautau (a).Whakarewahia te 0.5g o te matū ka tirohia ki te wai R ka waimeha ki te 10 mL me te whakarewa ano.
Te otinga whakamatautau (b).Ringihia te 2 mL o te wairewa whakamatautau (a) ki te 10 mL ki te wai R.
Te otinga tohutoro (a).Whakarewahia te 0.1 g o te histamine dihydrochloride CRS ki te wai R ka waimeha ki te 10 mL me te whakarewa ano.
Te otinga tohutoro (b).Whakarewahia te 50 mg o te histidine monohydrochloride R ki roto i te wai R ka waimeha ki te 100 mL me te whakarewa ano.
Te otinga tohutoro (c).Whakaranuhia te 1 mL o te otinga whakamatautau (a) me te 1 mL o te otinga tohutoro (b).
Hoatu ki te pereti 1 μL o te otinga whakamatautau (a), 1 μL o te otinga whakamatautau (b), 1 μL o te otinga tohutoro (a), 1 μL o te otinga tohutoro (b) me te 2 μL o te otinga tohutoro (c).Whakawhanakehia i runga i te ara 15cm ma te whakamahi i te ranunga o te 5 pukapuka haukini R, 20 rōrahi wai R me te 75 rōrahi acetonitrile R. Me whakamaroke te pereti ki te ia o te hau.Whakahokia te whanaketanga i te huarahi kotahi, whakamarokehia te pereti ki te hau ka rehu ki te wairewa ninhydrin R1.Whakawerahia te pereti i te 110°C mo te 10 meneti.Ko nga waahi e rite ana ki te histidine i roto i te chromatogram i whiwhi me te otinga whakamatautau (a) kaore i te kaha ake i te waahi o te chromatogram i whiwhi ki te otinga tohutoro (b) (1 paiheneti).Karekau te whakamatautau i te whaimana mena ka kitea e te chromatogram me te otinga tohutoro (c) e 2 nga waahi kua wehea marama.
Ngawhawha (2.4.13).Ko te 3mL o te wairewa S kua waimeha ki te 15 mL ki te wai makuku R e tutuki ana ki te whakamatautau tepe mo nga pūkawa (0.1 ōrau).
Ngaronga i te whakamaroketanga (2.2.32).Kaua e neke ake i te 0.5 paiheneti, ka whakatauhia ki te 0.20 g ma te whakamaroke i roto i te oumu i te 105 °C.
Te pungarehu ngongo (2.4.14).Kaua e neke ake i te 0.1 ōrau, kua whakatauhia ki te 0.5 g.
WHAKAMAHI
Whakarewahia te 0.080 g ki te ranunga o te 5.0 mL o te 0.01 M waikawa pūhaumāota me te 50 mL o te waipiro R. Kawea he titration potentiometric (2.2.20), ma te whakamahi i te 0.1 M konutai waikawa.Pānuihia te rōrahi i tāpirihia i waenga i ngā wāhi tuatahi me te tuatoru o te kōpikopiko.
1mL o te 0.1 M konutai hydroxideis he rite ki te 9.203 mg o C5H11Cl2N3.
PUTANGA
Rokiroki i roto i te ipu hau, parea i te marama.