L-Isoleucine CAS 73-32-5 (H-Ile-OH) Assay 98.5~101.0% Factory High Quality

L-Isoleucine (H-Ile-OH) (CAS: 73-32-5) AJI 97 Tikanga Whakamatau
Tautuhinga: Whakatauritea te awhiowhiotanga infrared absorption o te tauira ki tera o te paerewa ma te tikanga kōpae pāhare pāporo bromide.
Hurihuri Tau [α]20/D: Tauira maroke, C=4, 6mol/L HCl
State of Solution (Transmittance): 0.5g i roto i te 10ml o 1mol/L, HCl spectrophotometer, 430nm, 10nm matotoru pūtau.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml o 0.01mol/L HCl
Putakawa (SO4): 1.2g, (1), ref: 0.50ml o 0.005mol/L H2SO4
Rino (Fe): 1.5g, (1), ref: 1.5ml o Iron Std.(0.01mg/ml)
Nga Kourarahi (Pb): 2.0g, (1), memeha ma te whakamahana, ref: 2.0ml o Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (1), ref: 2.0ml o As2O3 Std.
Ētahi atu waikawa Amino: Tauira Whakamātautau: 50μg, B-6-a, mana whakahaere: L-Ile 0.25μg
Ngaronga i te whakamaroke: i te 105 ℃ mo te 3 haora.
Te whakamatautau: Tauira maroke, 130mg, (3), 3ml o te waikawa formic, 50ml o te waikawa ahetiki hukapapa, 0.1mol/L HCLO4 1ml=13.117mg C6H13NO2
Whakamātautau pH: 0.5g i te 20ml o H2O

L-Isoleucine (H-Ile-OH) (CAS: 73-32-5) USP35 Tikanga Whakamatau
WHAKAMAHI
Kei roto i te Isoleucine te NLT 98.5% me te NMT 101.5% o te L-Isoleucine (C6H13NO2), ka tatauhia i runga i te whakamaroke.
WHAKATAUTANGA
A. KAUPAPA INFRARED <197K>
WHAKAMAHI
WHAKAMAHI
Tauira: 130 mg o Isoleucine
Watea: Whakaranuhia te 3 mL o te waikawa formic me te 50 mL o te waikawa ahetiki hukapapa.
Pūnaha Titrimetric
(Tirohia Titrimetry <541>)
Aratau: Te titration tika
Titrant: 0.1 N te waikawa perchloric VS
Te kitenga ira mutunga: Potentiometric
Tātaritanga: Whakarewahia te Tauira ki te 3 mL o te waikawa formic me te 50mL o te waikawa ahetiki hukapapa.Titrate ki te Titrant.Mahia te whakatau Patea.
Tātaihia te ōrau o te Isoleucine (C 6H13NO2) i te Tauira i tangohia:
Hua = {[(VS-VB) x N x F]/W} x100
VS= Rōrahi titrant i pau i te Tauira (mL)
VB= Rōrahi titrant pau i te Pātea (mL)
N= te noho noa o te Titrant (mEq/mL)
F= tauwehe taurite, 131.2 mg/mEq
W= Tauira taumaha (mg)
Paearu whakaae: 98.5% ~ 101.5% i runga i te whakamaroke
NGA POKE
WHAKAMAHI TOHUTOHU <281>: NMT 0.3%
KORERO ME TE PUKA, KORERO <221>
Wairewa Paerewa: 0.50mL o te 0.020 N waikawa pūmāota
Tauira: 0.73g o Isoleucine
Paearu whakaae: NMT 0.05%
CHLORIDE AND SULFATE, Sulfate <221>
Wairewa paerewa: 0.10mL o te 0.020 N te waikawa pungatara
Tauira: 0.33g o Isoleucine
Paearu whakaae: NMT 0.03%
RINO <241>: NMT 30ppm
Mukua enei e whai ake nei:
NGA METAL TAITAHA, Tikanga I <231>: NMT 15ppm
NGA KAUPAPA KAUPAPA
Te otinga pai o te punaha: 0.4mg/mL ia USP L-Isoleucine RS me USP L-Valine RS i roto i te 0.1 N waikawa pūhaumāota
Te otinga paerewa: 0.05mg/mL o USP L-Isoleucine RS i roto i te 0.1 N waikawa pūmāota.[FAKATOKANGA-He kukū tenei otinga ki te 0.5% o te otinga Tauira.]
Tauira otinga: 10mg/mL o Isoleucine i roto i te 0.1 N waikawa pūmāota
Pūnaha Chromatographic (Tirohia Chromatography <621>, Chromatography Apa Angiangi.)
Aratau: TLC
Adsorbent: 0.25-mm te paparanga o te ranunga chromatographic silica gel
Rōrahi tono: 5μL
Te whakawhanake i te punaha whakarewa: Te waipiro Butyl, te waikawa acetic glacial, me te wai (3:1:1)
Reagent reagent: 2 mg/mL o te ninhydrin i roto i te ranunga o te waipiro butyl me te waikawa 2N acetic (95:5)
Te pai o te punaha
Nga whakaritenga e tika ana: E whakaatu ana te chromatogram o te otinga pai o te punaha e rua nga waahi kua wehea.
Tātaritanga
Tauira: Te otinga pai o te punaha, te otinga Paerewa, me te otinga Tauira.
I muri i te whakamaroke-rangi i te pereti, rehu ki te Reagent Reagent, ka whakamahana i waenga i te 100° me te 105° mo te 15 min.Tirohia te pereti i raro i te marama ma.
Paearu whakaae: Ko nga waahi tuarua o te otinga Tauira kaore i te nui ake, i te kaha ake ranei i te waahi matua o te otinga Paerewa.
He poke takitahi: NMT 0.5%
Tapeke poke: NMT 2.0%
NGA WHAKAMATAUTANGA
TE WHAKATAUTANGA OPTICAL, Te Hurihanga Motuhake <781S>
Tauira otinga: 40 mg/mL i roto i te 6 N waikawa pūhaumāota
Paearu whakaae: +38.9° ki +41.8°
PH <791>
Tauira otinga: 10 mg/mL otinga
Paearu whakaae: 5.5~7.0
NGARO I TE WHAKAMAROKE <731>: Me whakamaroke he tauira ki te 105 ℃ mo te 3 haora: ka ngaro te NMT 0.3% o tona taumaha.
HE WHAKAMAHI ATU
PAKITANGA ME TE WHAKAPUTANGA: Tiakina ki roto i nga ipu kua kati pai.
USP REFERENCE STANDARD <11>
USP L-Isoleucine RS
USP L-Valine RS