L-Leucine Methyl Ester Hydrochloride CAS 7517-19-3 (H-Leu-OMe·HCl) Whakamātautau >99.0% Factory

1. Te ahua
Tangohia te rahinga tika o tenei hua, hoatu ki runga pepa maeneene maeneene ka horahia te 5 cm2 me te 5 mm te matotoru.Tirohia i te waahi marama.
2. Pure (HPLC)
2.1 Taputapu
Ko te chromatography wai mahi teitei, te kaitirotiro PDA.
Taurite tātari hiko
2.2 Reagents
Acetonitrile (kōeke chromatographic), TFA(kōeke chromatographic)
2.3 Nga tikanga Chromatographic
2.3.1 Tīwae: YMC-ODS-AM, 5 μl, 150 x 4.6 mm
2.3.2
Te roangaru rapu: INC220nm
Rere rere: 1.0mL/min
Rōrahi werohanga:10 μL (mo te tohutoro)
Waiwai: Acetonitrile
Wā oma: 20 meneti
2.4 Whakaritenga wahanga pūkoro
Wāhanga pūkoro A (0.1% trifluoroacetic wai waikawa): te ngongo tika i te 2.0ml TFA, Dilute ki te 2000ml ki te wai, uru me te degas;
Wāhanga pūkoro B (0.1% trifluoroacetic acid acetonitrile ): te tango tika i te 2.0ml TFA, Dilute ki te 2000ml me te acetonitrile, te ranunga me te degas;
2.5 Tawhā taputapu
Wā (min) A% B%
0.00 90 10
12.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
2.6 Te whakarite tauira otinga
Paunatia me te whakakore i te tauira 0.1g me te acetonitrile, waimeha ki te 100ml, ruru pai hei whakamahi, kia rite ranei te kukū.Whakaritea kia rua nga tauira hei whakarara.
2.7 Tauira Whakatau
Tātarihia te tauira kia rite ki te tikanga tauira e whai ake nei:
Neke atu i te 1 werohanga o te otinga patea
1 wairewa tauira ngira 1#
1 wairewa tauira ngira 2#
2.8 Te Tatau Hua
2.8.1 I whakamahia te tikanga whakatikatika rohe tihi ki te tatau i te pokekore o te HPLC ma te tango i te waahi tuwhera.
2.8.2 Kaua e nui ake i te 1%.
2.8.3 Mena ka tutuki nga hua o nga werohanga e rua ki nga paearu whakaae, ka riro te maatanga toharite hei hua whakamutunga.
3, Ira whakarewa -- RY-1 taputapu ira whakarewa
4. Ngaronga i runga i te whakamaroke tikanga whakamatautau
4.1 Taputapu:
Umu whakamaroke thermostatic hiko, 1/10,000 toenga.
4.2 Tikanga:
I roto i te pounamu pauna he rite tonu te taumaha me te whakamaroke o te waha o te waha o te whenua, paunatia te 1 karamu (tika ki te 0.0001 karamu) o te tauira.Ko te tauira me horahia ki raro o te pounamu pauna me te matotoru kaua e neke atu i te 10mm, ka maka ki roto i te oumu whakamaroke hiko thermostatic, whakamaroke i te 105 ~ 110 ℃ mo te 3 haora, ka neke ki roto i te ruma whakamaroke ki te whakamatao te pāmahana rūma mo te pauna.
Tātaitanga: Ngaronga i te whakamaroketanga %= (M1-M2) ÷M×100
Kei hea: M1: te taumaha o te tauira me te pounamu ine i mua i te whakamaroke, karamu
M2: te taumaha o te tauira me te pounamu ine i muri i te whakamaroketanga, ki te karamu
M: Tauira taumaha, karamu
5. Te hurihanga motuhake
-- Ka inehia te hurihanga motuhake i runga i te GB/T613-1988
Te whakarite tauira: 1.0000g tauira i ata paunatia, ka nekehia ki te pounamu volumetric 50ml ma, maroke hoki, 20ml wai ka tapirihia, ka kapi te pounamu ka ruia kia memeha, katahi ka diluted ki te tauine ki te wai.
Whakamātautau: Whakaritea te kore o te gyroscope i mua i te whakamatautau, ka utaina te ipuipu whakamatautau me te otinga tauira, tuhia te Koki hurihuri, ka tatau i te hurihanga motuhake o te tauira me te tauira e whai ake nei.
[а]D20 = (r×50) ÷ (L×W)
[a]D20: Te hurihanga whatu motuhake i te 20 ℃ o te otinga tauira
r: Ka kitea te hurihanga mata i te 20 ℃ mo te otinga tauira
50: Rōrahi o te otinga tauira kua rite (ml)
w: Tauira taumaha (g)
L: Roa ngongo hurihuri whatu (dm)