L-Tryptophan CAS 73-22-3 (H-Trp-OH) Assay 98.5~101.5% Factory High Quality

L-Tryptophan (H-Trp-OH) (CAS: 73-22-3) AJI 97 Tikanga Whakamatau
Tautuhinga: Whakatauritea te awhiowhiotanga infrared absorption o te tauira ki tera o te paerewa ma te tikanga kōpae pāhare pāporo bromide.
Hurihuri Tau [α]20/D: Tauira maroke, C=1, H2O memeha ma te whakamahana
State of Solution (Transmittance): 0.5g i roto i te 20ml o 2 mol/L HCl, spectrophotometer, 430nm, 10mm te matotoru pūtau.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml o 0.01mol/L HCl
Haukinia (NH4): B-2
Putakawa (SO4): 1.2g, (1), ref: 0.50ml o 0.005mol/L H2SO4
Rino (Fe): 0.75g, (2), ref: 1.5ml o Iron Std.(0.01mg/ml)
Nga Kourarahi Taumaha (Pb): 2.0g, (4), ref: 2.0ml o Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (1), ref: 2.0ml o As2O3 Std.
Ētahi atu waikawa Amino: H2O, Whakarewa ma te whakamahana (40 ℃) Tauira Whakamātautau: 50μg, A-6-a, mana: L-Trp 0.25μg
Ngaronga i te whakamaroke: i te 105 ℃ mo te 3 haora.
Te toenga i runga i te mura (Sulfated): AJI Test 13
Te whakamatautau: Tauira maroke, 200mg, (1), 3ml o te waikawa formic, 50ml o te waikawa ahetiki hukapapa, 0.1mol/L HCLO4 1ml=20.423mg C11H12N2O2
Whakamātautau pH: 1.0g i te 100ml o H2O

L-Tryptophan (H-Trp-OH) (CAS: 73-22-3) USP35 Tikanga Whakamatau
WHAKAMAHI
Kei roto i te Tryptophan te NLT 98.5% me te NMT 101.5% o C11H12N2O2, hei L-Tryptophan, i tatauhia i runga i te tikanga maroke.
WHAKATAUTANGA
A. KAUPAPA INFRARED <197K>
WHAKAMAHI
WHAKAMAHI
Tauira otinga: Tuhia te 200 mg o Tryptophan ki roto i te ipu 125-mL.Whakarewa i roto i te ranunga o te 3 mL o te waikawa formic me te 50 mL o te waikawa ahetiki hukapapa.
Tātari: Titrate ki te 0.1 N te waikawa perchloric VS, te whakatau i te pito mutunga.Whakaritehia he whakatau patea, ka whakatikahia (tirohia Titrimetry <541>).Ko ia mL o te 0.1 N te waikawa perchloric he rite ki te 20.42 mg o C11H12N2O2.
Paearu whakaae: 98.5% ~ 101.5% i runga i te whakamaroke
NGA POKE
Nga poke parakore
WHAKAMAHI TOHUTOHU <281>: NMT 0.1%
CHLORIDE AND SULFATE, Chloride <221>: Ko te wahanga 0.73-g e whakaatu ana kaore e nui ake te chloride ki te 0.50 mL o te 0.020 N waikawa pūhaumāota (0.05%).[FAKATOKANGA-Me whakamahana marietia te tauira whakarite kia memeha, ki te tika.]
CHLORIDE AND SULFATE, Sulfate <221>: Ko te wahanga 0.33-g e whakaatu ana kaore e nui ake te sulfate i te rite ki te 0.10 mL o te 0.020 N waikawa pungatara (0.03%).[FAKATOKANGA-Me whakamahana marietia te tauira whakarite kia memeha, ki te tika.
RINO <241>: NMT 30ppm
NGA METAL TAHA, Tikanga II <231>: NMT 15ppm
Nga poke pararopi
WHAKAMAHI 1
Rongoā A: Te waikawa Trifluoroacetic i roto i te wai (1 mL/L)
Rongoā B: Te waikawa trifluoroacetic i roto i te wairewa acetonitrile me te wai (80:20) (1 mL/L wairewa waikawa trifluoroacetic)
Wairewa paerewa: 1.0 mg/L ia USP Tryptophan Related Compound A RS me USP Tryptophan Related Compound B RS i roto i te wai
Tauira otinga: 10.0 mg/mL o tryptophan i roto i te wai
Te otinga pai o te punaha: 1.0 mg/L o te USP Tryptophan Related Compound B RS i roto i te wai
Wāhanga pūkoro: Tirohia te ripanga rōnaki i raro nei.

Pūnaha Chromatographic (Tirohia Chromatography <621>, Pūnaha Whakaritenga.)
Aratau: LC
Kaitirotiro: UV 220 nm
Tīwae: 4.6-mm × 25-cm;5-µm tākai L1
Te pāmahana tīwae: 30°
Rere rere: 1 mL/min
Rahi werohanga: 20 µL
Te pai o te punaha
Tauira: Te otinga pai o te punaha
Te whakaritenga e tika ana
Inenga paerewa patata: NMT 5.0%
Tātaritanga
Tauira: otinga Paerewa me te otinga Tauira
Tātaihia te ōrau o ia poke kāore i tohua i roto i te wāhanga o Tryptophan i tangohia:
Hua = (rU/rS) × (CS/CU) × 100
rU = te waahi teitei o ia poke kore i tohua i roto i te otinga Tauira
rS = te horahanga teitei o te puhui e pa ana ki te tryptophan B i te otinga Paerewa
CS = te kukū o te USP Tryptophan Pānga Pūhui B RS i te otinga Paerewa (µg/mL)
CU = te kukū o Tryptophan i te Tauira otinga (µg/mL)
Paearu whakaae
Te tapeke poke 1: NMT 0.01% o te tapeke o nga poke ka rere i mua i te tihi tryptophan.
Te tapeke poke 2: NMT 0.03% o te tapeke o nga poke e rere ana i muri i te tihi tryptophan.[FAKATOKANGA-Whakakorehia te tihi mo te puhui whai take tryptophan B.]
Puhui whai take tryptophan A: Ki te kitea he tihi mo te puhui whai tryptophan A i roto i te Tauira otinga, katahi ka mahia te whakamatautau mo te Tikanga 2: Te Tepe o te Puhui Paa Tryptophan A, kei raro iho nei.
KAUPAPA 2: TE TEITI O TE PUU WHAI PAA A TRYPTOPHAN A
Otinga A: 18 mM konutai konutai phosphate 18 mM, kua tātarihia, kua whakakorehia (pH 2.5), me te acetonitrile (9:1)
Otinga B: 10 mM konutai konutai phosphate 10 mM, kua tātarihia, kua whakakorehia te hau (pH 2.5), me te acetonitrile (1:1)
Rongoā C: Acetonitrile i te wai (7:3)
Wairewa paerewa: 0.1 mg/L o te USP Tryptophan Related Compound A RS i roto i te wai
Tauira otinga: 10.0 mg/mL o Tryptophan i roto i te wai
Wāhanga pūkoro: Tirohia te ripanga rōnaki i raro nei

Pūnaha Chromatographic
(Tirohia te Chromatography <621>, Whakaritenga Pūnaha.)
Aratau: LC
Kaitirotiro: UV 216 nm
Tīwae: 3.9-mm × 15-cm;5-µm tākai L1
Te pāmahana tīwae: 30°
Rere rere: 1 mL/min
Rahi werohanga: 20 µL
Te pai o te punaha
Tauira: otinga Paerewa
Te whakaritenga e tika ana
Inenga paerewa patata: NMT 5.0%
Tātaritanga
Tauira: otinga Paerewa me te otinga Tauira
Tātaihia te ōrau o te pūhui whai pānga tryptophan
A i roto i te waahanga o Tryptophan i tangohia:
Hua = (rU/rS) × (CS/CU) × 100
rU = te horahanga teitei o te pūhui A i roto i te Tauira otinga
rS = te waahi teitei o te puhui A i roto i te otinga Paerewa
CS = te kukū o te USP Tryptophan Pūhui Pātahi A RS i te otinga Paerewa (µg/mL)
CU = te kukū o Tryptophan i te Tauira otinga (µg/mL)
Paearu whakaae: NMT 10 ppm
NGA WHAKAMATAUTANGA
TE WHAKATAUTANGA OPTICAL, Te Hurihanga Motuhake <781S>: -29.4° ki te -32.8°
Tauira otinga: 10 mg/mL, i roto i te wai.[FAKATOKANGA-Whakawera marie kia memeha, ki te tika.]
PH <791>: 5.5~7.0, i roto i te otinga (1 i roto i te 100)
NGARO I TE WHAKAMAROKE <731>: Me whakamaroke he tauira ki te 105° mo te 3 haora: ka ngaro te NMT 0.3% o tona taumaha.
HE WHAKAMAHI ATU
PAKITANGA ME TE WHAKAPUTANGA: Tiakina ki roto i nga ipu kua kati pai
USP REFERENCE STANDARD <11>
USP L-Tryptophan RS
USP Tryptophan Related Compound A RS
3,3′-[Ethylidenebis(1H-indole-1,3-diyl)]bis[2S)-2-aminopropanoic] waikawa.C24H26N4O4 432.49
USP Tryptophan Pūhui Pānga B RS
2-Acetamido-3-(1H-indol-3-yl)propanoic acid.C13H14N2O3 246.3