L-(-)-Tyrosine CAS 60-18-4 (H-Tyr-OH) Assay 98.5~101.5% Factory High Quality

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) AJI 97 Tikanga Whakamatau
Tautuhinga: Whakatauritea te awhiowhiotanga infrared absorption o te tauira ki tera o te paerewa ma te tikanga kōpae pāhare pāporo bromide.
Hurihuri Tau [α]20/D: Tauira maroke, C=5, 1mol/L HCl
State of Solution (Transmittance): 0.5g i te 10ml o 1mol/L HCl, spectrophotometer, 430nm, 10mm te matotoru pūtau.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml o 0.01mol/L HCl
Haukinia (NH4): B-2
Putakawa (SO4): 1.2g, (1), ref: 0.50ml o 0.005mol/L H2SO4
Rino (Fe): 1.5g, (2), ref: 1.5ml o Iron Std.(0.01mg/ml)
Nga Kourarahi Taumaha (Pb): 2.0g, (2), ref: 2.0ml o Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (2), ref: 2.0ml o As2O3 Std.
Ētahi atu waikawa amino: dilute haukini R2 Tauira Whakamātautau: 50μg, F-6-a, mana: L-Tyr 0.25μg
Ngaronga i te whakamaroke: i te 105 ℃ mo te 3 haora.
Te toenga i runga i te mura (Sulfated): AJI Test 13
Te whakamatautau: Tauira maroke, 180mg, (1), 6ml o te waikawa formic, 50ml o te waikawa ahetiki hukapapa, 0.1mol/L HCLO4 1ml=18.119mg C9H11NO3
Whakamātautau pH: 0.02g i roto i te 100ml wairewa wai kukū

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) USP35 Tikanga Whakamatau
WHAKAMAHI
Kei roto i te Tyrosine te NLT 98.5% me te NMT 101.5% o te L-Tyrosine (C9H11NO3), ka tatauhia i runga i te whakamaroke.
WHAKATAUTANGA
A. KAUPAPA INFRARED <197K>
WHAKAMAHI
WHAKAMAHI
Tauira: 180 mg o Tyrosine
Watea: Whakaranuhia te 6 mL o te waikawa formic me te 50 mL o te waikawa ahetiki hukapapa.
Pūnaha Titrimetric
(Tirohia Titrimetry <541>)
Aratau: Te titration tika
Titrant: 0.1 N te waikawa perchloric VS
Te kitenga ira mutunga: Potentiometric
Tātaritanga: Whakarewahia te Tauira ki te 6 mL o te waikawa formic me te 50mL o te waikawa acetic glacial, ka titrate ki te Titrant.Mahia te whakatau Patea.
Tātaihia te ōrau o te tyrosine (C9H11NO3) i roto i te Tauira i tangohia:
Hua = {[(VS-VB) x N x F]/W} x100
VS= rōrahi o te Titrant i pau i te Tauira (mL)
VB= rōrahi o te Titrant i pau i te Patea (mL)
N= te noho noa o te Titrant (mEq/mL)
F= tauwehe taurite, 181.2 mg/mEq
W= Tauira taumaha (mg)
Paearu whakaae: 98.5% ~ 101.5% i runga i te whakamaroke
NGA POKE
MAHI RANGI <281>: NMT 0.4%
KORERO ME TE PUKA, KORERO <221>
Wairewa Paerewa: 0.20mL o te 0.020 N waikawa pūmāota
Tauira: 0.35 g Tyrosine.[FAKATOKANGA-Ki te tika, memeha te Tauira ma te whakamahana kia tata te koropupu me te tapiri 1 mL waikawa nitric.]
Paearu whakaae: NMT 0.04%
CHLORIDE AND SULFATE, Sulfate <221>
Te otinga paerewa: 0.50mL o te 0.020 N te waikawa pungatara
Tauira: 1.2 g Tyrosine.[FAKATOKANGA-Mehemea e tika ana, memeha te Tauira ma te tapiri 6 mL o te waikawa puota wai.]
Paearu whakaae: NMT 0.04%
RINO <241>: NMT 30ppm [FAKATOKANGA-Mena e tika ana, whakamahia te 2 mL o te waikawa maaota ki te whakakore i te Tauira.]
NGA METAL TAITAHA, Tikanga I <231>: NMT 15ppm
NGA KAUPAPA KAUPAPA
Te wairewa haukini kua waimeha: Whakamehahia te 16 mL o te haukini kukū ki te wai ki te 100 mL.
Te otinga pai o te punaha: Whakarewahia te 10 mg o te USP L-Tyrosine RS me te 10 mg o te USP L-Phenylalanine RS i roto i te 1 mL o te wairewa haukini kua Diluted, ka waimeha ki te wai ki te 25.0 mL.
Wairewa Paerewa: I roto i te ipu e tika ana, whakamemeha i te rahinga o te USP L-Tyrosine RS ki te 1 mL o te wairewa haukinia Diluted.Waimeha ki te wai ki te rōrahi kia puta he otinga 0.05mg/mL.[FAKATOKANGA-He kukū tenei otinga ki te 0.5% o te otinga Tauira.]
Tauira otinga: Whakawhitia te 0.1 g o Tyrosine ki roto i te ipu 10-mL, ka tapirihia he wairewa haukini kua waimeha kia memeha, ka waimeha ki te wai ki te rōrahi.
Pūnaha Chromatographic (Tirohia Chromatography <621>, Chromatography Apa Angiangi.)
Aratau: TLC
Adsorbent: 0.25-mm te paparanga o te ranunga chromatographic silica gel
Rōrahi tono: 5μL
Te whakawhanake i te punaha whakarewa: Isopropyl alcohol and ammonium hydroxide (7:3)
Reagent reagent: 2 mg/mL o te ninhydrin i roto i te ranunga o te waipiro butyl me te waikawa 2N acetic (95:5)
Te pai o te punaha
Nga whakaritenga e tika ana: E whakaatu ana te chromatogram o te otinga pai o te punaha e rua nga waahi kua wehea.
Tātaritanga
Tauira: Te otinga pai o te punaha, te otinga Paerewa, me te otinga Tauira.
Me whakamaroke te pereti i waenga i te 100° me te 105° kia ngaro rawa atu te haukini.Rehu ki te Reagent Reagent, ka whakamahana i waenga i te 100° me te 105° mo te 15 min.Tirohia te pereti i raro i te marama ma.
Paearu whakaae: Ko nga waahi tuarua o te otinga Tauira kaore i te nui ake, i te kaha ake ranei i te waahi matua o te otinga Paerewa.
Tetahi poke takitahi: NMT 0.5%
Tapeke poke: NMT 2.0%
NGA WHAKAMATAUTANGA
TE WHAKATAUTANGA OPTICAL, Te Hurihanga Motuhake <781S>
Tauira otinga: 50 mg/mL i roto i te 1 N waikawa pūmāota
Paearu whakaae: -9.8° ki -11.2°
NGARO I TE WHAKAMAROKE <731>: Me whakamaroke he tauira ki te 105 ℃ mo te 3 haora: ka ngaro te NMT 0.3% o tona taumaha.
HE WHAKAMAHI ATU
PAKITANGA ME TE WHAKAPUTANGA: Tiakina ki roto i nga ipu kua kati pai.
USP REFERENCE STANDARD <11>
USP L-Phenylalanine RS
USP L-Tyrosine RS