Taputapu: GC taputapu (Shimadzu GC-2010)
Tīwae: DB-17 Agilent 30mX0.53mmX1.0μm
Te pāmahana oumu tuatahi: 80 ℃
Wā tuatahi 2.0min
Reiti 15℃/min
Te pāmahana oumu whakamutunga: 250 ℃
Te wa whakamutunga 20min
hau kawe hau hauota
Aratau Rere tonu
Rere 5.0mL/min
Ritua ōwehenga 10:1
Te pāmahana injector: 250 ℃
Te pāmahana kaitirotiro: 300 ℃
rōrahi werohanga 1.0μL
Ko nga mahi whakatupato i mua i te tātaritanga:
1. Whakaritea te pou i te 240 ℃ mo te 30 meneti iti rawa.
2. Horoi tika i te syringe me te injector liner ki te tango i nga poke o te tātaritanga o mua.
3. Horoi, whakamaroke ka whakakiia te waimeha ki roto i nga ipu horoi syringe.
Whakaritenga waimeha:
Whakaritea 2% w/v wairewa konutai waihā ki te wai.
Whakaritenga Paerewa:
Paunatia te 100mg o te paerewa (R)-3-hydroxyprrolidine hydrochloride ki roto i te ipu, taapirihia te 1mL o te waimeha ka rewa.
Whakaritenga whakamatautau:
Paunatia te 100mg o te tauira whakamatautau ki roto i te ipu, taapirihia te 1mL o te waimeha ka rewa.Whakaritea kia takirua.
Tikanga:
Werohia kia patea (whakawa), whakarite paerewa me te whakarite whakamatautau ma te whakamahi i nga tikanga o runga ake o te GC.Kaua e aro ki nga tihi na te mea kua patea.Ko te wa pupuri o te tihi na te (R) -3-hydroxyprrolidine e tata ana ki te 5.0min.
Tuhipoka:
Pūrongohia te hua hei toharite
Wā tuku: Nov-13-2021