Phthalimide Potassium Salt CAS 1074-82-4 Purity >99.0% (HPLC)

3. Tikanga whakamatautau
3.1 Ngaronga i te whakamaroke (W/W)
3.1.1 Nga taputapu me nga taputapu
Kotahi neke atu i te tekau mano o te toenga hiko, te oumu whakamaroke thermostatic hiko, 40*25 pounamu pauna, whakamaroke
Reagent: hiera hiraka
3.1.2 Tikanga
1. Horoi 3 40*25 ipu pauna.
2, hoatu ki roto i te oumu i te 105 ℃ (± 2 ℃) maroke mo te 1 haora.
3. Tangohia te pounamu pauna 40*25 ka maka ki roto i te whakamaroke kia whakamatao mo te 30min.
4, Tika tika 40 * 25 ine pounamu kau taimaha W0.
5. Whakamahia he pounamu pauna 40*25 hei pauna i te tauira whakananu rite o te 3.0000g (W1), he 3 katoa.
6, Whakanohia ki roto i te oumu, tangohia te potae pounamu, hoatu ki te taha o te pounamu pauna, ki te haurua ranei te whakatuwhera i te potae pounamu mo te whakamaroke, tangohia, me pai te pauna o te potae pounamu, kia maroke i te 105 ℃ (± 2 ℃) mo 3 haora.
7. Tangohia te pounamu pauna 40*25 ka hoatu ki roto i te taputapu whakamaroke kia whakamatao mo te 30min.
8, Te pauna tika i muri i te whakamaroketanga 40 * 25 te taimaha o te pounamu W2.
tātai tātai: Ngaronga i te whakamaroketanga (%) =(W1-W2)/(W1-W0)×100%
3. 4 Te whakatau kaupapa
3.4.1 Nga taputapu me nga taputapu
LC-UV100 Liquid Chromatograph (Shanghai Wufeng Scientific Instrument Co., LTD.)
10ml pounamu volumetric, koko kokonga kowiri tira me etahi atu whakaurunga e pa ana
3.3.2 Tikanga
Tikanga mo te tātaritanga o te Phthalimide Potassium Salt
3.3.3 Koromatography wai
Te ahua o te Chromatographic
Tīwae Chromatographic: HypersilC6H5 (C8 kei te wātea hoki) 4.6*250mm 5um
Te roangaru rapu: 210nm
Te pāmahana tīwae: 30℃
Rere rere: 1.0ml/min
Tauira kukū: 0.05mg/ml (diluent is the mobile phase)
Reagents
Pāhare pāhare Phosphate Dibasic(parakore tātari)
Phthalimide Potassium Salt (tauira paerewa)
Acetonitrile (parakore tātari)
Te wai parakore
Waahanga pūkoro: acetonitrile: phosphate buffer (PH:3.5) =40:60
Paunatia te 1.63g Potassium Phosphate Dibasic ka memeha ki te 600ml wai parakore, whakatikahia te uara PH ki te 3.5 me te waikawa phosphoric, ringihia ki roto i te 1000ml ipurau kei roto 400ml acetonitrile, uru pai, tātari ki te 0.45um i konatunatua ki te muka tātari membrane, rewa me te vibration ultrasonic. tono.
3.3.4 Tikanga
1) Te otinga whakamatautau whirihoranga (0.05mg/ml)
I ata paunatia te tauira 2.5mg ka maka ki roto i te pounamu volumetric 50ml.Ko te wahanga pūkoro i tapirihia me te diluted ki te tauine na roto i te whakakorenga ultrasonic.
2) otinga paerewa whirihoranga (1.0mg/ml)
Ko te tauira paerewa 2.5mg i ata paunatia ka hoatu ki roto i te pounamu volumetric 50ml.Ko te wahanga pūkoro i tapirihia me te diluted ki te tauine na roto i te whakakorenga ultrasonic.
3) Te whakamahinga o te punaha:
(1) Kuhua te upoko tātari ki roto i te 80% methanol i te tiimatanga, whakatuwheratia te puera whakamomori me te whakamakuku mo te 3-5min, ka kati i te rewharewha ka horoi mo te 10-15min.Na ka maka te upoko tātari ki roto i te 90% te wai parakore, whakatuwheratia te paera wairere me te whakamakuku mo te 3-5min, kati te paera wairere ka horoi mo te 10min.
(2) Na ka huri i te waahanga waea kua rite, ko te rere o te rere ko 1.0ml / min, ko te pehanga o mua o te pou he iti iho i te 1000Psi, whakatuwheratia te kaitirotiro, tatari kia mau te pehanga o mua o te pou, whakatuwhera i te teihana mahi rorohiko, ka uru ki te ipurangi.
(3) I muri i te u o te pehanga o mua o te pou, whakatuwheratia te kaitirotiro.Tuatahi werohia 1 ngira o te wahanga pūkoro, ka werohia kia 1 ngira o te tauira paerewa, ka mutu ka werohia te tauira.
(4) Nga hua tatau: tikanga whakarite rohe
(5) I muri i te whakaoti i te mahi tātari, kati tuatahi te kaitirotiro, ka horoi i te upoko tātari ki te wai parakore mo te 10-15min;I te wa ano, ka horoia te paera werohanga ki te wai me te methanol.
(6) Ka mutu, ka mau ki te 80% methanol, ka horoi mo te 30 meneti, ka kati.
4. I muri i te otinga o te tirotiro, whakakiia te tirotiro me te pepa whakamatautau taiwhanga.