Adenosine CAS 58-61-7 Assay 99.0% -101.0% USP Standard Factory High Purity

TANTHAUZO
Adenosine ili ndi NLT 99.0% ndi NMT 101.0% ya C10H13N5O4, yowerengedwa pa zouma.
CHIZINDIKIRO
• A. KUYAMBIRA KWA INFRARED <197K>: NMT 0.1%
ZOYESA
• ADENOSINE
Chitsanzo: 200 mg wa Adenosine poyamba zouma pa 105 ° kwa 2h
Titrimetric system
(Onani Titrimetry <541>)
Mode: Direct titration
Titrant: 0.1 N pa chloric acid VS
Kuzindikira komaliza: Potentiometric
Kusanthula: Sungunulani mu 50 mL wa glacial acetic acid ndi titrate ndi 0.1 N pa chloric acid VS.Werengani peresenti ya Adenosine (C10H13N5O4) pagawo lomwe mwatenga:
Zotsatira = [(V − B) × N × F × 100]/W
V = Voliyumu ya titrant (mL)
B = voliyumu yopanda kanthu (mL)
N= chizolowezi chokhazikika (mEq/mL)
F= chinthu chofanana: 267.25 mg/mEq
W = kulemera kwa Zitsanzo (mg)
Njira zolandirira: 99.0% -101.0% pazowuma
• ZOCHITSA
• KUYATSA KWA ZINTHU ZONSE <281>: NMT 0.1%
• ZINTHU ZONSE, Njira II <231>: NMT 10 ppm
• MALIRE A AMMONIA
Yankho lachitsanzo: Imitsani 0,5 g mu 10 ml ya madzi.Onetsetsani kwa 30 s, ndikudutsa mu fyuluta yolimba.Sungunulani filtrate ndi madzi mpaka 15 ml, ndipo gwiritsani ntchito kusefera.
Njira yothetsera: 0.4 µg/mL ya ammonium chloride m'madzi
Kusanthula: Pachitsanzo cha yankho ndi njira Yokhazikika yonjezerani 0,3 mL ya alkaline mer curic-potassium iodide TS, ikani machubu oyesera, ndikusiyani kwa mphindi zisanu.
Njira zolandirira: Yankho lachitsanzo silimawonetsa mtundu wachikasu kwambiri kuposa wa Standard solution (NMT 4 ppm of ammonia).
• MALIRE A CHLORIDE
Yankho lachitsanzo: Imitsani 0,2 g mu 10 ml ya madzi.Sakanizani kwa 30 s, dutsani fyuluta yowoneka bwino, ndipo gwiritsani ntchito kusefera.
Njira yothetsera: 2.3 µg/mL ya sodium chloride m'madzi
Kusanthula: Pachitsanzo cha yankho ndi 10 mL ya muyeso wa Standard onjezerani 1 mL wa nitric acid ndi 1 mL wa silver nitrate TS, ndi kusungunula njira iliyonse ndi madzi mpaka 40 ml.Lolani kuti mayankho aime kwa mphindi 5, kutetezedwa ku kuwala.
Njira zolandirira: Mukayang'ana kumbuyo kwakuda, yankho lachitsanzo silili lopanda phokoso kuposa yankho lokhazikika (NMT 0.007% chloride).
• MALIRE A SULFATE
Yankho lachitsanzo: Imitsani 0,75 g mu 15 ml ya madzi.Sakanizani kwa 30 s, dutsani fyuluta yowoneka bwino, ndipo gwiritsani ntchito kusefera.
Njira yokhazikika: Onjezani 0,15 mL ya 0.020 N sulfuric acid ku 15 mL yamadzi.
Kusanthula: Pachitsanzo cha yankho ndi njira Yokhazikika onjezerani 2 mL ya barium chloride TS ndi 1 mL ya 3 N hydrochloric acid, sungunulani njira iliyonse ndi madzi mpaka 30 ml, ndikusakaniza.Lolani kuti mayankho aime kwa 5 min.
Njira zolandirira: Yankho lachitsanzo ndilopanda chipwirikiti kuposa yankho lokhazikika (NMT 0.02% sulfate).
• ZINTHU ZOYENERA ZINTHU
Yankho A: 6.8 g/L ya potassium hydrogen sulfate ndi 3.4g/L ya tetrabutylammonium hydrogen sulfate m’madzi.Sinthani ndi 2 N potaziyamu hydroxide kukhala pH ya 6.5.
Yankho B: 0.1 g/L ya sodium azide solution
Gawo lam'manja: Yankho A ndi Njira B (60:40)
Njira yothetsera kuyenerera: 0.2 mg/mL iliyonse ya Adenosine ndi inosine mu gawo la Mobile
Chitsanzo yankho: 1.0 mg/mL wa Adenosine mu Mobile gawo
Chromatographic system
(Onani Chromatography <621>, Kukwanira Kwadongosolo.)
Njira: LC
Chowunikira: UV 254 nm
Mzere: 4.6-mm × 25-cm;5-µm kunyamula L1
Kuthamanga: 1.5 mL / min
Kukula kwa jekeseni: 20µL
Kuyenerera kwadongosolo
Zitsanzo: Njira yothetsera kuyenerera kwadongosolo
Zofunikira zoyenera
Kusamvana: NLT 9.0 pakati pa adenosine ndi inosine
Zomwe zimapangidwira: NMT 2.5
Kupatuka kofananira: NMT 2.0%
[Dziwani-Chromatograph yankho lachitsanzo, ndikusintha nthawi yothamanga kuti ikhale yosachepera kawiri nthawi yosungira pachimake chachikulu.]
Kusanthula
Chitsanzo: Yankho lachitsanzo
Werengani kuchuluka kwa zodetsa zilizonse mu gawo la Adenosine lomwe latengedwa:
Zotsatira = (rU/rT) × 100
rU = kuyankha kwakukulu kwa zodetsa zilizonse kuchokera ku Sample solution
rT = kuchuluka kwa mayankho onse pansonga zonse kuchokera muchitsanzo cha yankho
Zovomerezeka zovomerezeka
Zonyansa zapayekha: NMT 0.1% iliyonse ya guanosine, inosine, ndi uridine, ndi NMT 0.2% ya adenine
Zonyansa zonse: NMT 0.5%
MAYESO AKE
• KUSINKHA KAPENA KUCHULUKA <741>: 233°-238°
• KUCHULUKA KWA MAONE, Kusinthasintha Mwachindunji <781S>: -68° mpaka -72°
Njira yothetsera mayeso: 20 mg/mL mu sodium hydroxide solution (1 mu 20), yotsimikizika pa chitsanzo chowumitsidwa kale pa 105 ° kwa 2h
ACIDITY KAPENA KUKHALA KWAMBIRI: Imitsani 1 g mu 20 ml ya madzi opanda mpweya woipa.Onetsetsani kwa 30 s, ndikudutsa mu fyuluta yolimba.Pagawo lililonse la magawo 10-mL a kusefera onjezerani 0.1mL ya bromocresol yofiirira TS.
Njira zolandirira: NMT 0.3 mL ya 0.01 N sodium hydroxide imafunika kuti ipange mtundu wa blue-violet mugawo limodzi.NMT 0.1 mL ya 0.01 N hydrochloric acid ikufunika kuti ipange mtundu wachikasu mu gawo lina.
•KUYANUKA KWA LOSSON <731>: Yanikani chitsanzo pa 105℃ kwa 2 h: imataya NMT 0.5% ya kulemera kwake.
ZOWONJEZERA ZOFUNIKA
•KUPAKA NDI KUKUSIKA: Sungani m’miyendo yothina, yosamva kuwala, ndipo sungani m’zipinda zozizira bwino.
• MFUNDO ZA USP REFERENCE <11>
USP Adenosine RS