4-Amino-5-Chloro-2,3-Dihydrobenzofuran-7-Carboxylic Acid CAS 123654-26-2 Prucalopride Succinate Intermediate Purity ≥98.0% (HPLC)

Mankhwalawa ndi 4-Amino-5-Chloro-2,3-Dihydrobenzofuran-7-Carboxylic Acid, zomwe zili mu C12H12ClNO4 sizikhala zosachepera 98.0%, zowerengedwa pa maziko a anhydrous.
[Katundu] Chogulitsachi ndi choyera mpaka chofiyira.
[Chongani]
Tengani mlingo woyenera wa mankhwalawa (pafupifupi 0.5g) wa madzi, onjezerani zosungunulira (methanol: N,N-dimethylformamide = 50:50), ndi kuzisungunula molingana ndi njira yodziwira madzi (Zowonjezera Ⅷ M, njira yoyamba A).Gwiritsani ntchito madontho a mayeso a Fisher kuti muwone mpaka yankho litasintha kuchoka pachikasu chopepuka kupita kufiyira-bulauni zomwe zikuwonetsa kumapeto, ndikuyesanso kuyesa kwina.Werengetsani motsatira ndondomeko zotsatirazi: madzi okhutira sayenera kupitirira 1.00%.
Chinyezi (%) = (AB) F/W×100%
Pomwe, A ndi kuchuluka kwamadzi a Fisher omwe amadyedwa ndi chinthu choyesera (ml);
B ndi kuchuluka kwa madzi oyesera a Fisher omwe amadyedwa ndi malo opanda kanthu, ml;
F ndi kulemera kwa njira yoyesera ya Fisher yofanana ndi madzi pa 1ml, mg;
W ndi kulemera kwa chinthu choyesera, mg
Zogwirizana nazo
Malinga ndi njira yomwe ili pansi pa "chinthu choyezera zomwe zili", ngati pali nsonga zodetsa mu chromatogram ya yankho loyesa, gawo lachidebe chimodzi siliyenera kupitilira nthawi 10 (1.0%) la gawo lalikulu lachiwongolero. yankho, ndipo chiŵerengero cha nsonga ya zonyansa zonse sichidzakhala chachikulu kuposa nthawi za 20 (2.0%) za malo apamwamba kwambiri a njira yothetsera.
[Kutsimikiza Kwazinthu] Malinga ndi mawonekedwe apamwamba amadzimadzi chromatography (Zowonjezera VI D).
Octadecylsilane yomangika silika gel idagwiritsidwa ntchito ngati chodzaza mikhalidwe ya chromatographic ndi mayeso oyenerera dongosolo.Phosphate bafa njira (1.36g potaziyamu dihydrogen mankwala, kusungunuka akupanga mu 1L madzi, phosphoric asidi kusintha PH 3.0) ankagwiritsidwa ntchito monga mafoni gawo A. Acetonitrile ndi mafoni gawo B, ndi gradient elution ikuchitika motsatira tebulo:
Nthawi (min) Mobile phase A mobile phase B
0 8020
15 8020
30 50 50
45 50 50
Kutalika kwa mawonekedwe kunali 254 nm.Kuthamanga kunali 1.0 ml / min.Kuchuluka kwa mbale zongoyerekeza ndi zosachepera 2000 malinga ndi nsonga ya 4-Amino-5-Chloro-2,3-Dihydrobenzofuran-7-Carboxylic Acid
Kuchuluka kwa furanoformic acid sikuchepera 2000.
Njira yotsimikizira:
Tengani 10mg ya mankhwala oyezetsa, muyeze molondola, muyike mu botolo la 10ml, sungunulani akupanga ndi gawo la mafoni A-mobile gawo B (70:30) monga zosungunulira, tsitsani pa sikelo, gwedezani bwino, ndikukonzekera yankho ndi ndende ya 1mg/ml.20 l yolondola idabayidwa mu chromatograph yamadzimadzi, ndipo chromatogram idalembedwa.Chiyero chachikulu chapamwamba sichinali chochepera 98.0% molingana ndi njira yokhazikika yamalo okhazikika
Mzere: Dionex Acclaim C18, 4.6 * 250mm, 5um
Kutentha kwapakati: 40 ℃
Kuthamanga: 1.0ml / min
Kutalika kwa mawonekedwe: 254nm
Kukula kwachitsanzo: 10μL