L-Glutamic Acid α-Benzyl Ester CAS 13030-09-6 (H-Glu-OBzl) Mayeso >98.5% (HPLC) High Quality

1. Purity (HPLC)
1.1 Zida
High performance liquid chromatography, UV3000 chowunikira.
Electronic analytical balance
1.2 Reagents
Acetonitrile (chromatographic grade), TFA (chromatographic grade)
1.3 Mikhalidwe ya Chromatographic
Mzere wa 1.3.1: Mzere wa C18, 5 μl * 250 * 4.6 mm
1.3.2 Kutalika kwa mawonekedwe: 220nm
Kuthamanga: 1.0 mL / min
Voliyumu ya jakisoni: 10 μL (zofotokozera)
Zosakaniza: acetonitrile
Nthawi yothamanga: Mphindi 20
1.4 Kukonzekera gawo la mafoni
Mobile gawo A (0.1% Trifluoroacetic acid Madzi): molondola kuyamwa 1.0 ml TFA kuchepetsedwa 1000 ml ndi madzi, mix ndi degas.
Mobile gawo B (0.1% Trifluoroacetic acid Acetonitrile): molondola kuyamwa 1.0 ml TFA, kuchepetsedwa 1000 ml ndi acetonitrile, mix ndi degas.
Pulogalamu ya 1.5 Gradient
Nthawi (mphindi) A% B%
0:00 90 10
10.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
1.6 Kukonzekera chitsanzo cha yankho
Yezerani 0.1 g chitsanzo ndikusungunula ndi acetonitrile ndikuchepetsa mpaka 100ml.Gwedezani bwino kuti mugwiritse ntchito m'tsogolo, kapena ndende yomweyo.Konzani zitsanzo ziwiri zofanana.
1.7 Kutsimikiza Kwachitsanzo
Sankhulani chitsanzocho motsatira njira zotsatirazi:
1) Kupitilira 1 jekeseni wa yankho lopanda kanthu
2) 1 jakisoni wa chitsanzo yankho 1#
3) 1 jakisoni wa chitsanzo yankho 2#
1.8.0 Kuwerengera
1.8.1 Njira yokhazikika yamalo okhazikika idagwiritsidwa ntchito kuwerengera chiyero cha HPLC.
1.8.2 Chibale chikutanthauza kupatuka kwa chiyero cha singano ziwiri sikuyenera kukhala kwakukulu kuposa 1%
1.8.3 Ngati zotsatira za majekeseni onsewa zikugwirizana ndi kuvomereza, tengani chiyero chapakati monga chotsatira chomaliza.