Chenodeoxycholic Acid (CDCA) CAS 474-25-9 Assay ≥98% (Dry Basic)

Chenodeoxycholic Acid
C24H40O4: 392.57
3a,7a-Dihydroxy-5b-cholan-24-oic acid [474-25-9]
Chenodeoxycholic Acid, ikauma, imakhala yosachepera 98.0z komanso osapitirira 101.0% ya C24H40O4.
Kufotokozera Chenodeoxycholic Acid imapezeka ngati yoyera, makhiristo, ufa wa crystalline kapena ufa.
Amasungunuka momasuka mu methanol ndi ethanol (99.5), sungunuka mu acetone, ndipo pafupifupi osasungunuka m'madzi.
Chizindikiritso Dziwani kuchuluka kwa mayamwidwe a infrared a Chenodeoxycholic Acid, omwe adawumitsidwa kale, monga momwe adalangizidwira mu njira ya potassium bromide disk pansi pa Infrared Spectrophotometry <2.25>, ndikufanizira mawonekedwe ndi Reference Spectrum: mawonekedwe onsewa amawonetsa kulimba kofanana kwa mayamwidwe amtundu womwewo.
Kusinthasintha kwa kuwala <2.49> [a]20D : +11.0 °~ +13.0 ° (pambuyo kuyanika, 0.4 g, ethanol (99.5), 20 mL, 100 mm).
Malo osungunuka <2.60> 164 ~ 169 ℃
Chiyero (1) Chloride <1.03>-Sungani 0.36 g wa Chenodeoxycholic Acid mu 30 ml ya methanol, onjezerani 10 mL wa dilute nitric acid ndi madzi kuti mupange 50 mL, ndikuyesani ndi yankho ili.Konzani njira yothetsera motere: Pa 1.0 mL ya 0.01 mol/L hydrochloric acid VS onjezerani 30 ml ya methanol, 10 ml ya dilute nitric acid ndi madzi kuti mupange 50 ml (osapitirira 0.1%).
(2) Zitsulo zolemera <1.07>-Pitirizani ndi 1.0 g ya Chenodeoxycholic Acid molingana ndi Njira 4, ndikuchita mayeso.Konzani njira yowongolera ndi 2.0 mL ya Standard Lead Solution (osapitirira 20 ppm).
(3) Barium-Ku 2.0 g wa Chenodeoxycholic Acid onjezerani 100 ml ya madzi, ndi wiritsani kwa mphindi ziwiri.Pa yankho ili onjezerani 2 mL wa hydrochloric acid, wiritsani kwa mphindi ziwiri, fyuluta mukaziziritsa, ndikutsuka fyulutayo ndi madzi mpaka mutenge 100 ml ya sefa.Pa 10 ml ya kusefera onjezerani 1 mL ya sulfuric acid yosungunuka: palibe chipwirikiti chomwe chikuwonekera.
(4) Zinthu zogwirizana-Sungani 0,20 g ya Chenodeoxycholic Acid mu osakaniza acetone ndi madzi (9: 1) kuti mupange ndendende 10 mL, ndikugwiritsa ntchito njira iyi ngati njira yothetsera.Payokha, sungunulani 10 mg wa lithocholic acid kwa chromatography yopyapyala mu osakaniza acetone ndi madzi (9: 1) kuti mupange ndendende 10 ml.Chitoliro cha 2 ml cha yankho ili, onjezerani chisakanizo cha acetone ndi madzi (9: 1) kuti mupange ndendende 100 ml, ndipo gwiritsani ntchito njira iyi ngati yankho lokhazikika (1).Payokha, sungunulani 10 mg wa ursodeoxycholic acid mu osakaniza acetone ndi madzi (9: 1) kuti apange 100 ml ndendende, ndipo gwiritsani ntchito yankho ili ngati yankho lokhazikika (2).Payokha, sungunulani 10 mg ya cholic acid ya chromatography yopyapyala mu osakaniza acetone ndi madzi (9: 1) kuti mupange ndendende 100 ml, ndikugwiritsa ntchito yankho ili ngati yankho lokhazikika (3).Chitoliro cha 1 ml ya yankho lachitsanzo, ndipo yikani chisakanizo cha acetone ndi madzi (9: 1) kuti mupange 20 ml ndendende.Chitoliro 0,5 mL, 1 mL, 2 mL, 3 mL ndi 5 mL ya yankho ili, onjezerani kusakaniza kwa acetone ndi madzi (1) kwa aliyense wa iwo kuti apange 50 ml ndendende, ndipo tchulani njirazi ngati yankho lokhazikika A, yankho lokhazikika. B, njira yokhazikika C, yankho lokhazikika D ndi yankho lokhazikika E, motsatana.Chitani mayeso ndi mayankho awa monga momwe akulembera pa Thin-layer Chromatography<2.03>.Malo 5 ml iliyonse ya yankho lachitsanzo, mayankho wamba (1), (2), (3) ndi mayankho wamba A, B, C, D ndi E pa mbale ya silika gel osakaniza chromatography.Pangani mbale ndi osakaniza 4-methyl-2-pentanone, toluene ndi formic acid (16: 6: 1) kwa mtunda wa pafupifupi 15 cm, airdry mbale, ndi zina youma pa 1209C kwa mphindi 30.Nthawi yomweyo, utsi wogawana yankho la phosphomolybdic acid n-hydrate mu ethanol (95) (1 mu 5) pa mbale, ndi kutentha pa 120 ℃ kwa mphindi 2 mpaka 3: malo olingana ndi malo omwe ali ndi yankho lokhazikika (1) silokulirapo kuposa malo omwe ali ndi yankho lokhazikika (1), malo ogwirizana ndi malo omwe ali ndi yankho lokhazikika (2) silambiri kuposa malo omwe ali ndi yankho lokhazikika (2), komanso malo ogwirizana ndi malowo. ndi yankho lokhazikika (3) silili lamphamvu kuposa malo omwe ali ndi yankho lokhazikika
(3).Poyerekeza ndi mawanga omwe ali ndi mayankho okhazikika A, B, C, D ndi E, mawanga ena kupatula malo oyamba ndi ena kupatula mawanga omwe tawatchulawa sali ochulukirapo kuposa malo omwe ali ndi yankho la E, komanso kuchuluka kwake. mwa iwo saposa 1.5%.
Kutaya pa kuyanika <2.41> Osapitirira 1.5% (1 g, 105 ℃,3 maola).
Zotsalira pakuyatsa <2.44> Osapitirira 0.1% (1 g).
Kuyeza Kuyeza molondola za 0.5 g wa Chenodeoxycholic Acid, zouma kale, kusungunula mu 40 mL wa ethanol (95) ndi 20 mL madzi, ndi titrate <2.50> ndi 0.1 mol/L sodium hydroxide VS (potentiometric titration).Chitani chitsimikiziro chopanda kanthu mwanjira yomweyo, ndipo pangani kukonza kulikonse kofunikira.
ML iliyonse ya 0.1 mol/L sodium hydroxide VS ＝ 39.26 mg ya C24H40O4
Zotengera ndi zosungirako Zotengera-Zolimba zolimba.