Chloramphenicol CAS 56-75-7 Purity ≥99.0% (HPLC) High Purity

Chloramphenicol
C11H12Cl2N2O5 323.13 [56-75-7].
Chloramphenicol ili ndi zosachepera 97.0 peresenti komanso zosaposa 103.0 peresenti ya C11H12Cl2N2O5.
Kupaka ndi kusunga-Sungani zotengera zothina.
Kulembera -Kumene kuyenera kugwiritsidwa ntchito popanga jekeseni kapena mitundu ina ya jekeseni wosabala, chizindikirocho chimanena kuti ndi wosabala kapena chiyenera kukonzedwanso panthawi yokonzekera jekeseni kapena mitundu ina ya jekeseni wosabala.
Miyezo yolozera ya USP <11>-
USP Chloramphenicol RS Dinani kuti muwone Kapangidwe
USP Endotoxin RS
Chizindikiritso-
A: Infrared mayamwidwe <197K>.
B: Nthawi yosungira pachimake chachikulu mu chromatogram ya Kukonzekera kwa Assay ikufanana ndi yomwe ili mu chromatogram ya Kukonzekera kwa Standard, monga momwe zapezedwa mu Assay.
Kusungunuka <741>: pakati pa 149 ndi 153.
Kusinthasintha kwachindunji <781S>: pakati pa +17.0 ndi +20.0.
Njira yothetsera mayeso: 50 mg, osawumitsidwa, pa mL, mu mowa wopanda madzi.
Crystallinity <695>: imakwaniritsa zofunikira.
Mabakiteriya endotoxins 85-Kumene Chloramphenicol amapangidwira kuti agwiritsidwe ntchito pokonzekera mafomu a jekeseni, imakhala ndi 0.2 USP Endotoxin Unit pa mg wa chloramphenicol.
Sterility 71- Kumene lebulo likunena kuti Chloramphenicol ndi yobala, imakwaniritsa zofunikira ikayesedwa monga momwe zalembedwera Kusefera kwa Membrane pansi pa Kuyesedwa kwa Kusabereka kwa Zinthu kuti Kuwunikidwe, kupatula kugwiritsa ntchito 1 g ya chitsanzo cholimba.
pH <791>: pakati pa 4.5 ndi 7.5, mu kuyimitsidwa kwamadzi komwe kuli 25 mg pa mL.
Chromatographic purity-Sungani mlingo woyezera bwino wa Chloramphenicol mu methanol kuti mupeze njira yoyesera yomwe ili ndi 10 mg pa mL.Konzani njira yothetsera USP Chloramphenicol RS mu methanol yokhala ndi 10 mg pa mL (Standard solution A).Sungunulani magawo a Standard solution A mochulukira ndi methanol kuti mupeze yankho lokhazikika B lomwe lili ndi 100 µg pa mL ndi njira yokhazikika C yokhala ndi 50 µg pa mL.Ikani magawo osiyana a 20-µL a yankho loyesa ndi mayankho Okhazikika B ndi C ku mbale yoyenera yopyapyala yopyapyala (onani Chromatography 621), yokutidwa ndi wosanjikiza wa 0.25-mm wa chromatographic silika gelisi osakaniza.Pangani chromatogram mu zosungunulira zokhala ndi chloroform, methanol, ndi glacial acetic acid (79:14:7) mpaka kutsogolo kwa zosungunulira kusuntha pafupifupi magawo atatu mwa anayi a utali wa mbaleyo.Chotsani mbaleyo mchipindacho, chowuma, ndikuwunika pansi pa kuwala kwa UV kwaufupi: malo aliwonse kupatulapo malo oyambira omwe atengedwa kuchokera ku yankho la mayeso sadutsa kukula kapena kulimba kwa malo oyamba omwe atengedwa ku Standard solution B (1% ), ndi kuchuluka kwa zonyansa zomwe zimayimiridwa ndi madontho onse kupatulapo malo oyamba, kutengera kulimba kwa malo oterowo ndi kulimba kwa mawanga akuluakulu opezeka ku Standard Solutions B ndi C, sikudutsa 2% .
Kuyesa-
Mobile phase-Konzani osakaniza oyenera osefedwa madzi, methanol, ndi glacial acetic acid (55:45:0.1).Sinthani ngati kuli kofunikira (onani Kukwanira Kwadongosolo pa Chromatography 621).
Kukonzekera kokhazikika- Sungunulani kuchuluka kwake koyezedwa bwino kwa USP Chloramphenicol RS mu gawo la Mobile, ndikuchepetsa mochulukira, komanso pang'onopang'ono ngati kuli kofunikira, ndi gawo la Mobile kuti mupeze yankho lomwe lili ndi ndende yodziwika pafupifupi 80 µg pa mL.Sefa gawo lina la yankholi kudzera mu fyuluta ya 0.5-µm kapena porosity yabwino kwambiri, ndipo gwiritsani ntchito kusefera komveka bwino monga kukonzekera Kwanthawi zonse.
Kukonzekera kuyesa- Tumizani pafupifupi 200 mg ya Chloramphenicol, yoyezedwa molondola, ku botolo la volumetric 100-mL, kuwonjezera gawo la Mobile ku voliyumu, ndikusakaniza.Tumizani 4.0 mL ya yankho ku botolo la volumetric 100-mL, tsitsani ndi gawo la Mobile mpaka voliyumu, ndikusakaniza.Sefa gawo lina la yankholi kudzera mu fyuluta ya 0.5-µm kapena porosity yabwino kwambiri, ndipo gwiritsani ntchito kusefa komveka bwino monga kukonzekera Assay.
Chromatographic system (onani Chromatography <621>) - Chromatograph yamadzimadzi imakhala ndi chowunikira cha 280-nm ndi ndime ya 4.6-mm × 10-cm yomwe ili ndi 5-µm packing L1.Kuthamanga kwa madzi ndi pafupifupi 1 ml pa mphindi.Chromatograph the Standard kukonzekera, ndikujambulitsa mayankho apamwamba monga momwe adayankhidwira mu Ndondomeko: magwiridwe antchito omwe amatsimikiziridwa kuchokera pachimake cha analyte sichochepera 1800 mbale zongoyerekeza, chinthu chamchira sichikupitilira 2.0, ndipo kupatuka kwachibale kwa jakisoni wobwereza sikuli. kuposa 1.0%.
Ndondomeko--[chidziwitso-Gwiritsani ntchito nsonga zapamwamba pomwe mayankho apamwamba akuwonetsedwa.] Lowetsani padera ma voliyumu ofanana (pafupifupi 10 µL) a Kukonzekera kwa Standard ndi kukonzekera kwa Assay mu chromatograph, lembani ma chromatogram, ndi kuyeza mayankho a nsonga zazikulu.Werengetsani kuchuluka, mu mg, wa C11H12Cl2N2O5 mu gawo la Chloramphenicol lotengedwa ndi chilinganizo:
2.5C(rU / rS)
momwe C ndi ndende, mu µg pa mL, ya USP Chloramphenicol RS mu Kukonzekera Kwanthawi zonse, ndipo rU ndi rS ndiwo mayankho apamwamba omwe apezedwa kuchokera ku Kukonzekera kwa Assay ndi kukonzekera Kwanthawi zonse, motsatana.