EDC · HCl CAS 25952-53-8 Coupling Reagent Purity > 99.0% (T) Factory

1-(3-Dimethylaminopropyl) -3-Ethylcarbodiimide Hydrochloride (EDC · HCl) (CAS: 25952-53-8) ndi ufa woyera wa crystalline, wosasunthika, wosungunuka m'madzi, wosungunuka mu Mowa, womwe umagwiritsidwa ntchito ngati activating reagent ya carboxyl. magulu mu kaphatikizidwe ka amide, amagwiritsidwanso ntchito poyambitsa magulu a phosphate, kulumikizana pakati pa mapuloteni ndi ma nucleic acid, ndikupanga ma immunocouplers.EDC-HCl imagwiritsidwa ntchito mu pH ya 4.0-6.0 ndipo nthawi zambiri imagwiritsidwa ntchito pamodzi ndi N-Hydroxysuccinimide (NHS) kapena N-Hydroxysulfosuccinimide mchere wa sodium kuti apititse patsogolo kugwirizana bwino.
EDC·HCl ndi carbodiimide yosungunuka m'madzi, imagwiritsidwa ntchito kwambiri polumikiza peptide.EDC·HCl imagwiritsidwa ntchito ngati carboxyl activate agent.Zimagwira ntchito yofunika kwambiri pakupangitsa kuti ma biomolecules akulu asasunthike polumikizana ndi N-hydroxysuccinimide.Amagwiritsidwanso ntchito mu acylation ya phosphoranes.
Chochokera kumadzi chosungunuka cha carbodiimide chothandiza polumikizana ndi haptens ku mapuloteni ndi ma polypeptides.Amagwiritsidwa ntchito kusinthira ma receptors a NMDA komanso ngati cholumikizira mu peptide synthesis.Ubwino waukulu wa kulumikizana kwa EDAC ndikuchotsa kosavuta kwa reagent owonjezera ndi urea wofananira ndikutsuka ndi asidi kapena madzi.Carbodiimides amathandizira kupanga ma amide bond, carboxylic acid, ndi amines poyambitsa carboxylate kupanga O-acylurea.Wapakati uyu akhoza kuwukiridwa ndi amine mwachindunji kupanga amide.EDAC imatulutsidwa ngati chotengera chosungunuka cha urea.
EDC · HCl ikhoza kugwiritsidwa ntchito popanga ma amides.Amagwiritsidwa ntchito ngati cholumikizira pakupanga kwa esters kuchokera ku carboxylic acid pogwiritsa ntchito dimethylaminopyridine ngati chothandizira.
Pali zinthu zitatu zomwe zimagwiritsidwa ntchito nthawi zambiri: DCC, DIC ndi EDC·HCl.Kugwiritsiridwa ntchito kwa mtundu uwu wa condensing wothandizira nthawi zambiri kumafuna kuwonjezera kwa acylation catalysts kapena activators, monga DMAP, HOBt, ndi zina zotero. chothandizira sichigwiritsidwa ntchito kuti chitembenuzire kukhala ester yogwira ntchito kapena amide yogwira ntchito, chidzasinthidwa kukhala chotsatira chokhazikika cha urea (Njira B).Choyatsira condensation: Pali mitundu yotsatirayi ya ma condensation activator.Pakalipano, 4-N,N-dimethylpyridine (DMAP) yakhala ikugwiritsidwa ntchito kwambiri poyambitsa machitidwe osiyanasiyana a acylation.Nthawi zina 4-PPY ingagwiritsidwe ntchito ngati mphamvu ya DMAP si yabwino.Malinga ndi zolemba zoyenera, luso lake lothandizira ndilokwera pafupifupi nthawi chikwi kuposa DMAP.pakati pa ma condensers atatu omwe amagwiritsidwa ntchito kwambiri, DCC ndi DIC ndi otchipa.Nthawi zambiri DCC ndi DMAP amagwiritsidwa ntchito limodzi.Chimodzi mwazovuta zazikulu zogwiritsira ntchito DCC ndi chakuti mankhwala ena omwe amachitira, dicyclohexylurea, ali ndi solubility yaing'ono mu gawo la organic koma amasungunuka pang'ono, choncho n'zovuta kuchotsa bwino pogwiritsa ntchito njira zomwe zimagwiritsidwa ntchito poyeretsa, recrystallization, chromatography ndi zina zotero;chifukwa solubility wa dicyclohexylurea mu etha ndi wochepa kwambiri kuposa zosungunulira zina, Choncho, mankhwala a mtundu uwu wa zimene zimachititsa nthunzi nthunzi zimene zosungunulira ndi anawonjezera efa, ndi Zosefera ambiri a dicyclohexylurea pamaso zina mankhwala.Chifukwa diisopropylurea yopangidwa ndi DIC imakhala ndi kusungunuka kwabwinoko muzosungunulira organic, nthawi zambiri imagwiritsidwa ntchito pophatikiza chemistry solid-phase synthesis.EDCI pakali pano imagwiritsidwa ntchito kwambiri mu chemistry yamankhwala.Chimodzi mwazofunikira zake ndikuti urea yomwe imapangidwa pambuyo pakuchitapo kanthu imakhala yosungunuka m'madzi komanso yosavuta kutsukidwa.Nthawi zambiri, EDCI imaphatikizidwa ndi HOBt (zindikirani: izi HOBt nthawi zambiri imakhala yofunikira, apo ayi zitha kubweretsa zokolola zotsika kwambiri).Nthawi zina ngati chotchinga cha asidi chili chachikulu kapena pali gulu lochotsa ma elekitironi, zomwe zimachitika zimakhalabe mu sitepe yogwira ester (ester yogwira imakhala ndi sipekitiramu yolimba kwambiri ndipo imatha kuzindikirika ndi MS kapena LC-MS).