Fmoc-Leu-OH CAS 35661-60-0 N-Fmoc-L-Leucine Purity >99.0% (HPLC) Factory

Ndondomeko yoyendera
1. Maonekedwe
-- Kuwunika kowoneka
2. Purity (HPLC)
2.1 Chida
High performance liquid chromatography, PDA chowunikira.
Electronic analytical balance
2.2 Reagent
Acetonitrile (chromatographic grade), Trifluoroacetic acid (chromatographic grade)
2.3 Mikhalidwe ya Chromatographic
2.3.1 Mzere: YMC-ODS-AM, 5μL, 250x4.6 mm
2.3.2 Kutalika kwa mawonekedwe: INC220nm
Kuthamanga: 1.0mL / min
Kukula kwachitsanzo: 10μL (reference)
Zosakaniza: acetonitrile
Nthawi yosonkhanitsa deta: 25.00min
2.4 Kukonzekera gawo la mafoni
Mobile gawo A (0.1% trifluoroacetic acid madzi): molondola kuyamwa 2.0ml trifluoroacetic acid, kuchepetsedwa ndi madzi 2000ml, sakanizani bwino, ndi degassing;
Mobile gawo B (0.1% acetonitrile trifluoroacetic acid): molondola kuyamwa 2.0ml trifluoroacetic acid, kuchepetsedwa kwa 2000ml ndi acetonitrile, kusakaniza ndi degassing;
Nthawi (mphindi) A% B%
0.00 90 10
13.00 10 90
18.00 10 90
18.01 90 10
23.00 90 10
2.6 Kukonzekera chitsanzo cha yankho
Yesani ndi kusungunula chitsanzo cha 0.1g ndi acetonitrile ndi kuchepetsa mpaka 100ml, gwedezani bwino kuti mugwiritse ntchito, kapena ndende yomweyo.Konzani zitsanzo ziwiri zofanana.
2.7 Kutsimikiza Kwachitsanzo
Sankhulani chitsanzocho motsatira njira zotsatirazi:
Kupitilira 1 jekeseni wopanda kanthu
1 chitsanzo cha singano yankho 1#
1 singano chitsanzo yankho 2#
2.8 Kuwerengera zotsatira
2.8.1 Njira yokhazikika yamalo okhazikika idagwiritsidwa ntchito kuwerengera chiyero cha HPLC pochotsa malo opanda kanthu.
2.8.2 Chibale chikutanthauza kupatuka kwa chiyero cha singano ziwiri sikuyenera kukhala wamkulu kuposa 1%
2.8.3 Ngati zotsatira za majekeseni onsewa zikugwirizana ndi zovomerezeka, chiyero chapakati chimatengedwa ngati chotsatira chomaliza.
3, Malo osungunuka -- RY-1 chida chosungunuka
4. Kutayika pa njira yoyesera yowumitsa
4.1 Zida:
Ovuni yamagetsi ya thermostatic, 1/10,000 balance.
4.2 Ndondomeko:
Mu botolo loyezera lathyathyathya lokhala ndi kulemera kosalekeza ndi chivundikiro cha pakamwa chowuma kwambiri, yezani 1 gramu (yolondola mpaka 0.0001 gramu) ya chitsanzo.Zitsanzo ziyenera kufalikira pansi pa botolo lolemera ndi makulidwe osapitirira 10mm, kuyika mu uvuni wamagetsi wa thermostatic, wouma pa 105 ~ 110 ℃ kwa maola atatu, kenako ndikulowa m'chipinda choyanika kuti muziziritsa. kutentha kwa chipinda poyeza.
Kuwerengera: Kutaya pakuyanika %= (M1-M2) ÷M×100
Kumeneko: M1: kulemera kwa zitsanzo ndi botolo loyezera musanayanike, magalamu
M2: kulemera kwa zitsanzo ndi botolo loyezera mutatha kuyanika, mu magalamu
M: Kulemera kwachitsanzo, magalamu
5. Kuzungulira kwachindunji
-- Kuzungulira kwachindunji kumayesedwa molingana ndi GB/T613-1988
Kukonzekera kwachitsanzo: chitsanzo cha 0.5000g chinayesedwa bwino ndikusunthira ku botolo loyera ndi louma la 50ml volumetric, 20ml DMF inawonjezeredwa, botolo linatsekedwa ndikugwedezeka kuti lisungunuke, ndiyeno limachepetsedwa ku sikelo ndi DMF.
Mayeso: Sinthani zero ya gyroscope mayeso asanayesedwe, kenaka tsitsani chubu choyesera ndi yankho lachitsanzo, lembani ngodya yozungulira, ndikuwerengera kusinthasintha kwachitsanzo ndi formula iyi.
[а]D20=(r×50) ÷ (L×W)
[а]D20: Kuzungulira kwapadera kwapadera pa 25 ℃ ya yankho lachitsanzo
r: Kuzungulira kwa kuwala komwe kumawonedwa pa 20 ℃ kwa yankho lachitsanzo
50: Voliyumu yachitsanzo chokonzekera (ml)
w: kulemera kwachitsanzo (g)
L: Kutalika kwa chubu chozungulira (dm)