H-Glu(OEt)-OEt·HCl CAS 1118-89-4 L-Glutamic Acid Diethyl Ester Hydrochloride Purity>99.0% (HPLC) Factory

I. Njira zoyendera
1. Malo osungunuka
Chida cha Digital melting point chimagwiritsidwa ntchito
Gawo 2: Zamkatimu
2.1 Ma reagents
Gawo 1: Formic acid
2. Glacial acetic acid
3. Perchloric acid muyezo wa titration solution (C=0.1000mol/l)
4. Crystal Violet chizindikiro chamadzimadzi: 5g / l.
2.2 Zida ndi zida
1. Beaker 150ml
2. Microburet: 10ml
2. 3 Kusanthula njira
2. 3. Ndimalemera molondola za 300mg ya chitsanzo chowumitsidwa kale pa 105 ℃ kwa 3h, ndikuyiyika mu 150ml bethel, kuwonjezera 3ml ya formic acid, kuwonjezera 50ml ya ayezi acetic acid, kuwonjezera madontho a 2 a crystal violet test solution ( TS-74), ndi titrate ndi 0.1mol/L perchloric acid mpaka kumapeto kobiriwira kapena mpaka buluu litazimiririka.
2. 3. 2 Njira yowerengera
X ndi wofanana ndi C (V1-V2) nthawi 0.2042 / M nthawi 100
C ndi kuchuluka kwa perchloric acid muyezo wa titration solution, wofotokozedwa mu moles pa lita imodzi (mol/l).
V1 ndi voliyumu yogwiritsidwa ntchito ya perchloric acid standard titration concentrated solution mu zitsanzo za titration, zofotokozedwa mu ml (ml).
Volume yogwiritsidwa ntchito ya perchloric acid standard titration solution mu mayeso opanda kanthu a V2, owonetsedwa mu ml (ml)
0.2042 ndi 1.00ml perchloric acid muyezo titrated anaikira madzi gawo pamene khalidwe la mankhwala zikufotokozedwa mu G
M Ubwino wa chitsanzo, wofotokozedwa mu magalamu (g).
2. 4 Kuwongolera
Ngati kusiyana kwa kutentha kwa chipinda pakati pa yankho la titration test ndi calibration perchloric acid solution iposa 10 ℃, iyenera kusinthidwanso;Kupanda kutero, ndende ya C1 ya perchloric acid muyezo yothetsera iyenera kukonzedwa molingana ndi mayeso (2).
C2= C1/1+0.0011 (t1-t2)
1+0.0011 ndiye kuchuluka kwa glacial acetic acid
Chigawo cha kutentha kwa chipinda cha T2 calibration ya perchloric acid ndi Celsius (° C).
Chigawo cha kutentha kwa chipinda chomwe chitsanzo chimayikidwa ndi Celsius (° C).
C1 t2 ℃ perchloric acid muyezo yankho ndende, mu moles pa lita (MOL/L)
3. Zouma ndi zopanda kulemera
3.1 Zida:
Thermostatic kuyanika uvuni, 1/10,000 bwino.
3.2 Ndondomeko:
Tengani 1-2 magalamu a zitsanzo (zolondola mpaka 0.5mg) ndi kuziyika mu chisanadze zouma mosalekeza-kulemera ndi kulemera masekeli botolo.Zitsanzo zidzakhala ndi makulidwe ofanana pansi pa botolo loyezera.Ponena za zitsanzo, zidzawumitsidwa mu uvuni (105 ± 5 ℃) wa thermoelectric mpaka kulemera kosalekeza.
3.3 Kuwerengera
X ndi m kuchotsera m1 wogawidwa ndi m kuchotsa m2 kuchulukitsa 100
Kumene: X - chinyezi%
m1 - Kulemera kwa chitsanzo chowuma kuphatikiza botolo loyezera g
m2 - Yezerani kuchuluka kwa botolo g
m- Kulemera kwa chitsanzo g
3.4 Mfundo Zofunika Kusamala
1. Mfundo zofunikira: 1. Zitsanzo zofanana ziyenera kupangidwa kuti ziume ndi zolemera.Chitsanzo chimodzi chili ndi zotsatira ziwiri, ndipo cholakwika pakati pa zotsatira ziwiri sichiposa 0.5%.
2. Kuyeza kuyenera kukhala kosamala, kolondola mpaka 0.0001.
4 ku kuzungulira
4.1 Mfundo
Kuwala kopangidwa ndi mpweya kumadutsa mumadzimadzi kapena njira yapawiri yokhala ndi zinthu zina zowoneka bwino (ndiko kuti, chinthu chowoneka bwino) kungayambitse kuzungulira kwa kuwala, kupangitsa kuti kuwala kwa polarized kuzungulire kumanzere kapena kumanja.Kuchuluka kwa madigiri a kuzungulira kumatchedwa optical rotation.Pa kutentha kwina (kawirikawiri kuwonetsedwa ndi t, kungakhale 20 ℃ kapena 25 ℃) kuwala kwina kowala (kuwala kwachikasu kwa sodium ndi D, wavelength λ 589.3nm) kuwala kopangidwa ndi polarized kupyolera mu millilita iliyonse yomwe ili ndi 1g kuwala kwa zinthu, makulidwe a 1dm (ndiko kuti, 10cm) yankho pamene kusinthasintha kumatchedwa kusinthasintha kwapadera.
4.2 Zofunikira
Mzere wa D wa sodium spectrum (589. 3 nm) unagwiritsidwa ntchito kuyeza kutalika kwa chubu la 1dm ndipo kutentha kunali 20 ℃.
4.3 Zida
4.3.1 Digital autotropy (kulondola ± 0.001 °)
4.3.2 Electronic balance (kulondola 0.0001g).
4.4 Njira Yogwirira Ntchito
Tengani 3g ya mankhwalawa, yolondola mpaka 0.0001g, muyike mu botolo la 100ml volumetric, onjezerani Mowa kuti musungunuke ndi kusungunula ku sikelo, gwedezani bwino.Muzimutsuka chubu choyezera ndi madzi pang'ono kangapo, kenaka lowetsani madziwo pang'onopang'ono mu chubu choyezera (musapangitse thovu), ikani chubu choyezera mu gyroscope kuti muzindikire, ndikuwerenga kuwerenga kozungulira kwa kuwala. 3 nthawi mofanana.
4.5 Kuwerengera
Kuzungulira kwapadera kwa L-glutamate Diethyl carbonate kupita ku sodium spectrum D-line pa 20 ℃ [Alpha]D20 kunawerengedwa motere:
[ɑ] D20 = alpha/m
Kumene: α -- avareji ya chiwerengero cha ma kiyubiki kasinthasintha;
m -- chitsanzo khalidwe.
4.6 Kusiyana kovomerezeka: Kusiyana kotheratu kwa chitsanzo chomwecho sikuyenera kupitirira 0.02% poyesedwa kawiri.
4.7 Kusamala
4.7.1 Zosungunulira ziyenera kugwiritsidwa ntchito ngati kuwerengetsera opanda kanthu muyeso uliwonse: Mukayeza, muyesenso kuyenera kupangidwanso kuti muwone ngati ziro zisintha poyeza.Ngati pali kusintha kulikonse, kutembenuka kwa kuwala kwa chitsanzo kuyenera kutsimikiziridwa kachiwiri.
4.7.2 Chitsanzocho chiyenera kufotokozedwa pambuyo pa kusungunuka ndi kusungunuka;apo ayi, iyenera kusefedwa.
Gawo 5 Mawonekedwe
Chogulitsacho chizikhala Choyera mpaka choyera cha microcrystalline powder