Histamine Dihydrochloride CAS 56-92-8 Assay 98.5~101.0% (Titration) Factory

Histamine Dihydrochloride (CAS: 56-92-8) EP9.0 VolumeⅡNjira Yoyesera
TANTHAUZO
Histamine dihydrochloride ili ndi zosachepera 98.5 peresenti komanso zosaposa 101.0 peresenti ya 2-(1H-imidazol-4-yl) ethan-1-amine dihydrochloride, yowerengedwa potengera zinthu zouma.
AKALE
A woyera kapena pafupifupi woyera, crystalline ufa kapena colorless makhiristo, hygroscopic, kwambiri sungunuka m'madzi, sungunuka mowa.
CHIZINDIKIRO
Chizindikiritso choyamba: A, D.
Chizindikiritso Chachiwiri: B, C, D
A. Yang'anani ndi infrared absorption spectrophotometry (2.2.24), poyerekeza ndi sipekitiramu yopezedwa ndi histamine dihydrochloride CRS.Yang'anani ngati ma disks okonzedwa pogwiritsa ntchito 1 mg wa zinthu.
B. Yang'anani ma chromatogram omwe adapezeka pakuyesa kwa histidine.Malo ofunikira mu chromatogram yopezedwa ndi yankho la mayeso (b) ndi ofanana ndi malo, mtundu ndi kukula kwa malo oyambira mu chromatogram yopezedwa ndi yankho lolozera (a).
C. Sungunulani 0.1 g mu 7 mL wa madzi R ndi kuwonjezera 3 mL wa 200 g/L yothetsera sodium hydroxide R. Sungunulani 50mg wa sulfanilic acid R mu osakaniza 0.1 mL wa hydrochloric acid R ndi 10 mL madzi R ndi onjezerani 0,1 mL ya sodium nitrite solution R. Onjezerani yankho lachiwiri loyamba ndikusakaniza.Mtundu wofiira umapangidwa.
D. Amapereka mphamvu (a) ya kloridi (2.3.1).
MAYESO
Yankho S. Sungunulani 0.5g m'madzi opanda carbon dioxide R okonzedwa kuchokera ku madzi osungunuka R ndi kuchepetsa 10 mL ndi zosungunulira zomwezo.
Mawonekedwe a yankho.Yankho S ndi lomveka (2.2.1) ndipo silikhala lakuda kwambiri kuposa yankho la Y7 (2.2.2, Njira II).
pH (2.2.3).PH ya yankho S ndi 2.85 mpaka 3.60.
Histidine.Yang'anani ndi chromatography yopyapyala (2.2.27), pogwiritsa ntchito TLC silica gel G mbale R.
Njira yothetsera mayeso (a).Sungunulani 0,5g ya chinthucho kuti muwunike m'madzi R ndikusungunula mpaka 10 ml ndi zosungunulira zomwezo.
Njira yothetsera mayeso (b).Sungunulani 2 ml ya njira yoyesera (a) mpaka 10 ml ndi madzi R.
Yankho lolozera (a).Sungunulani 0,1 g wa histamine dihydrochloride CRS m'madzi R ndi kusungunula mpaka 10 mL ndi zosungunulira zomwezo.
Yankho lolozera (b).Sungunulani 50 mg wa histidine monohydrochloride R m'madzi R ndi kuchepetsa 100 mL ndi zosungunulira chomwecho.
Yankho lolozera (c).Sakanizani 1 ml ya yankho loyesa (a) ndi 1 ml ya yankho lolozera (b).
Ikani pa mbale 1 μL ya yankho la mayeso (a), 1 μL ya yankho loyesa (b), 1 μL ya yankho lazowunikira (a), 1 μL ya yankho lazowunikira (b) ndi 2 μL ya yankho lazowunikira (c).Pangani njira ya 15cm pogwiritsa ntchito chisakanizo cha 5volumes ya ndende ya ammonia R, mavoti 20 a madzi R ndi mavoti 75 a acetonitrile R. Yanikani mbaleyo mumlengalenga.Bwerezani chitukukocho mbali imodzi, yimitsani mbaleyo mozungulira mpweya ndikupopera ndi yankho la ninhydrin R1.Kutenthetsa mbale pa 110 ° C kwa mphindi 10.Malo aliwonse olingana ndi histidine mu chromatogram yopezedwa ndi mayeso oyesa (a) sali okulirapo kuposa malo omwe ali mu chromatogram yopezedwa ndi yankho (b) (1 peresenti).Kuyesako sikovomerezeka pokhapokha ngati chromatogram yopezedwa ndi yankho lolozera (c) ikuwonetsa mawanga awiri olekanitsidwa bwino.
Sulfates (2.4.13).3mL ya solution S yochepetsedwa kufika 15mL ndi madzi osungunula R imagwirizana ndi malire a sulfates (0.1 peresenti).
Kutaya pakuyanika (2.2.32).Osapitirira 0,5 peresenti, yotsimikizika pa 0,20 g poyanika mu uvuni pa 105 ° C.
Phulusa la Sulfated (2.4.14).Osapitirira 0,1 peresenti, yotsimikizika pa 0,5 g.
ZOYESA
Sungunulani 0.080 g mu osakaniza 5.0 mL wa 0.01 M hydrochloric acid ndi 50 mL mowa R. Chitani potentiometric titration (2.2.20), pogwiritsa ntchito0.1 M sodium hydroxide.Werengani voliyumu yomwe yawonjezeredwa pakati pa mfundo yoyamba ndi yachitatu ya kusinthasintha.
1mL ya 0.1 M sodium hydroxide ndi ofanana ndi 9.203 mg wa C5H11Cl2N3.
KUSINTHA
Sungani mu chidebe chopanda mpweya, chotetezedwa ku kuwala.