L-4-Nitrophenylalanine Methyl Ester HCl CAS 17193-40-7 H-Phe(4-NO2)-OMe·HCl Assay >98.5% (HPLC) Factory

Njira Yowunikira L-4-Nitrophenylalanine Methyl Ester HCl (H-Phe(4-NO2)-OMe·HCl) (CAS: 17193-40-7)
Kufotokozera: Ikani pafupifupi 2 g ya zitsanzo mu mbale yoyera ndi youma ya petri ndikuwona maonekedwe a chitsanzo.Kupereka lipoti: Nenani zomwe mwawona
Identificaiton: Fananizani nthawi yosungira pachimake chachikulu mu chromatogram yokonzekera mayeso ndi nthawi yosungira pachimake chachikulu mu chromatogram ya kukwanira kwa machitidwe monga momwe amapezera mu chromatographic purity ndi HPLC.
Kutaya pa Kuyanika: Yanikani botolo loyimitsidwa ndi galasi, losazama kwambiri pa 105±2℃ kwa mphindi 30.Lolani kuti izizizire mpaka kutentha kwa chipinda mu desciccator.Yezerani molondola botolo loyezera ndi choyimitsa ndipo lembani kulemera kwake (W1).Tumizani pafupifupi 1 mpaka 2g yoyezera molondola kuchuluka kwa zinthu zoyezera mu botolo loyezera.Mwa kugwedeza mofatsa, m'mbali, gawani chitsanzocho mofanana momwe mungathere mpaka kuya pafupifupi 5 mm.Yezerani botolo lomwe ladzaza ndi kulemba kulemera kwake (W2).Ikani botolo lodzaza mu uvuni wowumitsa, kuchotsa choyimitsa ndikuchisiyanso m'chipinda.Yanikani chitsanzo choyesera pa 105 ± 2 ℃ kwa maola atatu.Mukatsegula chipindacho, tsekani botolo mwamsanga, ndipo mulole kuti lifike kutentha kwa chipinda mu desiccator.Yezerani molondola botolo loyezera ndi choyimitsa ndikubwezeretsa kulemera kwake (W3).Yerezerani kuchuluka kwa kutayika pakuwumitsa sampuli pogwiritsa ntchito fomula ili:
Kuwerengera Kutaya pakuyanika (% w/w)= (W2-W3) ÷ (W2-W1) x 100
Enantiomer Content by HPLC:
Chemical & Reagents:
N-Hexane AR kalasi
Methanol HPLC kalasi
Isopropyl Alcohol HPLC kalasi
Diethylamine AR kalasi
Miyezo: L-4-Nitrophenylalanine Methyl Ester HCl muyezo;L-4-Nitrophenylalanine Methyl Ester HCl enantiomer muyezo
Mobile Phase: Konzani kusakaniza kofanana kwa n-Hexane, Isopropyl Alcohol, Methanol ndi Diethylamine (65:20:15:0.1 v/v/v/v)
Kukonzekera Kopanda kanthu: Chotsani 2.5ml ya Methanol mu botolo la 10-ml volumetric, chepetsani ku voliyumu ndi gawo la mafoni ndikusakaniza.
Kukonzekera koyenera kwadongosolo:
Yezerani molondola ndi kusamutsa pafupifupi 5 mg iliyonse ya L-4-Nitrophenylalanine Methyl Ester HCl muyezo ndi L-4-Nitrophenylalanine Methyl Ester HCl enantiomer muyezo mu 10-ml volumetric botolo, kusungunula pafupifupi 2.5ml wa methanol, kuchepetsa voliyumu ndi foni yam'manja. gawo ndi kusakaniza.
Kukonzekera mayeso:
Yezerani molondola ndikusamutsa pafupifupi 5 mg ya zitsanzo mu botolo la 10-mol volumetric, sungunulani pafupifupi 2.5ml ya methanol, chepetsani ku voliyumu ndi gawo la mafoni ndikusakaniza.
Zida zoimbira:
Chida: HPLC yokhala ndi jekeseni, Pampu, chowunikira cha UV ndi Chojambulira
Mzere: ChiralPak-IC (4.6mm x 250mm) 5 μ kapena zofanana
Kuthamanga: 1.0 ml / mphindi
Kutalika: 260nm
Kuchuluka kwa jekeseni: 10 μL
Kutentha kwapakati: 25 ℃
Nthawi yothamanga: Mphindi 20
Ndondomeko
Lowetsani njira zoyankhira monga zatchulidwira mundondomeko zotsatirazi.Lembani ma chromatogram ndikutsimikizira zoyenera kuchita:
Kufotokozera kwa Majekeseni Nambala ya Zofunikira za jakisoni
Kukonzekera Kopanda kanthu 1 Palibe nsonga yosokoneza yomwe iyenera kuwonedwa pa nthawi yosungira L-4-Nitrophenylalanine Methyl Ester HCl kapena enantiomer.
Kukonzekera koyenera kwa kachitidwe 1 Kukhazikika pakati pa L-4-Nitrophenylalanine Methyl Ester HCl ndi nsonga ya enantiomer sayenera kuchepera 2.0
Kukonzekera mayeso 1 Gwiritsani ntchito chromatogram yoyeserera kuti mudziwe za enantiomer muzachitsanzo.
Nthawi Zotsalira:
L-4-Nitrophenylalanine Methyl Ester HCl 1.0
Enantiomer Pafupifupi 1.11
Nthawi yosungira L-4-Nitrophenylalanine Methyl Ester HCl ndi pafupi mphindi 9.8.
Kuwerengera: Mu chromatogram yopezedwa pokonzekera mayeso, musanyalanyaze nsonga zonse kuyembekezera L-4-Nitrophenylalanine Methyl Ester HCl ndi Enantiomer.Nenani zomwe zili mu enantiomer mu zitsanzo ndi njira yosinthira malo.
Chromatogrpahic Purity and Impurities yolembedwa ndi HPLC:
Chemical & Reagents:
Diammonium phosphate AR kalasi
Phosphoric acid AR kalasi
Methanol HPLC kalasi
Madzi Oyeretsedwa
Miyezo
L-4-Nitrophenylalanine Methyl Ester HCl muyezo
4-Nitro-L-Phenylalanine monohydrate muyezo
Mobile Phase-A
Sungunulani 6.6 g wa Diammonium phosphate mu 1000ml wa madzi oyeretsedwa.Sinthani pH ya yankho ili kukhala 6.0 ndi Phosphoric acid.Sefa ndi degas.Musanayeze pH ya yankho, yang'anani pH mita ndi yankho lokhazikika la pH 4.01 NDI 7.00.
Mobile Phase-B
Methanol HPLC kalasi
Kukonzekera Koyenera Kwadongosolo
Kuyeza molondola ndi kusamutsa za 25 mg aliyense wa L-4-Nitrophenylalanine Methyl Ester HCl muyezo ndi 4-Nitro-L-Phenylalanine monohydrate muyezo mu 50-ml volumetric botolo, kupasuka ndi kuchepetsa voliyumu ndi methanol ndi kusakaniza.
Kukonzekera Mayeso:
Yesani molondola ndi kusamutsa pafupifupi 25 mg chitsanzo mu 50-ml volumetric botolo, sungunulani ndi kuchepetsa voliyumu ndi methanol ndi kusakaniza.
Zida zoimbira:
Chida: HPLC yokhala ndi jekeseni, Pampu, chowunikira cha UV ndi Chojambulira
Mzere: XTerra RP-18 (4.6mm x 150mm) 5 μ kapena zofanana
Kuthamanga: 1.0 ml / mphindi
Kutalika: 265nm
Kuchuluka kwa jekeseni: 20 μL
Kutentha kwapakati: 25 ℃
Nthawi yothamanga: 45 min
Pulogalamu ya Gradient
Nthawi (mphindi) Gawo Lam'manja-A % Gawo Lam'manja-B %
0.01 90 10
15 6040
35 40 60
40 35 65
41 90 10
45 90 10
Kachitidwe:
Lowetsani njira zoyankhira monga zatchulidwira mundondomeko zotsatirazi.Lembani ma chromatogram ndikutsimikizira zoyenera kuchita:
Kukonzekera Kopanda kanthu 1 Palibe nsonga yosokoneza iyenera kuwonedwa pa nthawi yosungira L-4-Nitrophenylalanine Methyl Ester HCl.
Kukonzekera koyenera kwa kachitidwe ka 1 Factor ya L-4-Nitrophenylalanine Methyl Ester HCl pachimake sayenera kukhala osachepera 2.0
Kukonzekera mayeso 1 Gwiritsani ntchito chromatogram yoyesera kuti mudziwe chiyero cha chromatographic ndi zonyansa.
Nthawi Zotsalira:
L-4-Nitrophenylalanine Methyl Ester HCl 1.0 (nthawi yosungira pafupifupi mphindi 11.4)
4-Nitro-L-Phenylalanine Pafupifupi 0.4
Toluene Pafupifupi 2.3
Kuwerengera:
Mu chromatogram yopezedwa pokonzekera mayeso, mutatha kukonza popanda kanthu, musanyalanyaze nsonga chifukwa cha toluene.Nenani za kuyera kwa chromatographic, 4-Nitro-L-Phenylalanine zomwe zili ndi zonyansa zonse muzachitsanzo ndi njira yokhazikika.